Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F256 I0 R1	328	102.7	5123138	93.4%	4785010	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,278,298	C→T	100%	intergenic (‑61/+272)	yejG ← / ← bcr	uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,278,298	0	C	T	98.9%	289.9 / ‑4.5	90	intergenic (‑61/+272)	yejG/bcr	uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (48/41); total (48/42)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

CAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTT  >  NC_000913/2278202‑2278394

cAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaac                                                                                               <  2:1722969/100‑1 (MQ=255)

  gTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaacaa                                                                                             >  1:1609893/1‑100 (MQ=255)

      aTGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCg                                                                                         <  1:162032/100‑1 (MQ=255)

       tGGACGATTGAGAGTTGTAACGAAGTCACTTATTTTCCCCGGAAGAACATTCACATCACAATTTGTTTACATCAATTTTAACCCATAATTAACACTCAGt                                                                                        >  2:2082024/1‑100 (MQ=255)

        ggACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTc                                                                                       >  1:670083/1‑100 (MQ=255)

         gACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCt                                                                                      >  1:2018720/1‑100 (MQ=255)

         gACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCt                                                                                      <  1:642747/100‑1 (MQ=255)

          aCGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCtt                                                                                     >  2:583188/1‑100 (MQ=255)

          aCGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCtt                                                                                     <  1:1939002/100‑1 (MQ=255)

            gATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTta                                                                                   <  1:1505864/100‑1 (MQ=255)

            gATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTta                                                                                   <  1:1865390/100‑1 (MQ=255)

             aTTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAAGAATCCGTCTTtat                                                                                  <  1:1742027/100‑1 (MQ=255)

             aTTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTtat                                                                                  >  1:168700/1‑100 (MQ=255)

               tGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATAc                                                                                >  1:687843/1‑100 (MQ=255)

                gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                               >  2:34207/1‑100 (MQ=255)

                gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                               >  1:213683/1‑100 (MQ=255)

                 agagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAg                                                                              >  2:1045015/1‑100 (MQ=255)

                  gagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGa                                                                             >  1:869240/1‑100 (MQ=255)

                  gagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGa                                                                             >  2:950051/1‑100 (MQ=255)

                    gTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATc                                                                           >  1:1152920/1‑100 (MQ=255)

                    gTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATc                                                                           >  1:2350575/1‑100 (MQ=255)

                    gTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATc                                                                           >  1:758575/1‑100 (MQ=255)

                     ttGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCg                                                                          >  2:2087806/1‑100 (MQ=255)

                     ttGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCg                                                                          <  1:1298673/100‑1 (MQ=255)

                      tGTAATGAAGTCACTTATTTTCCCCGGAAGCCCATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGt                                                                         >  2:522909/1‑100 (MQ=255)

                          aTGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAg                                                                     <  1:1045023/100‑1 (MQ=255)

                            gAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAt                                                                   <  1:833473/100‑1 (MQ=255)

                            gAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAt                                                                   <  2:2130198/100‑1 (MQ=255)

                               gTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGa                                                                <  2:187047/100‑1 (MQ=255)

                                 cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCCATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGAt                                                               >  2:128291/1‑99 (MQ=255)

                                 cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              <  2:911622/100‑1 (MQ=255)

                                 cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              >  1:510161/1‑100 (MQ=255)

                                 cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              >  1:901547/1‑100 (MQ=255)

                                 cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGATTTCATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              <  2:1797036/100‑1 (MQ=255)

                                   cTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGcc                                                            >  1:382978/1‑100 (MQ=255)

                                   cTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGcc                                                            >  1:2125964/1‑100 (MQ=255)

                                    ttATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCt                                                           >  1:1509886/1‑100 (MQ=255)

                                     tATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTc                                                          >  2:1098516/1‑100 (MQ=255)

                                       ttttCCCCTGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGc                                                        <  2:2031931/100‑1 (MQ=255)

                                       ttttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGc                                                        <  2:2193482/100‑1 (MQ=255)

                                         ttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCt                                                      >  1:2414107/1‑100 (MQ=255)

                                            cccGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGc                                                    >  2:589389/1‑99 (MQ=255)

                                             ccGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGt                                                  >  1:1952401/1‑100 (MQ=255)

                                              cGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTg                                                 <  2:329723/100‑1 (MQ=255)

                                               ggAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTgg                                                >  2:376245/1‑100 (MQ=255)

                                                gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGc                                               <  2:1234499/100‑1 (MQ=255)

                                                gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGc                                               <  1:1069586/100‑1 (MQ=255)

                                                   gCACATTCCCTTCACAATGTGTTTACCTCAATTTTAACACATCATTAACAATCCGTCTTTACACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGcc                                              <  2:1530761/98‑1 (MQ=255)

                                                   gCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctc                                            <  2:1601466/100‑1 (MQ=255)

                                                       aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                        <  1:79194/100‑1 (MQ=255)

                                                       aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                        >  1:1191993/1‑100 (MQ=255)

                                                       aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                        >  2:794001/1‑100 (MQ=255)

                                                       aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                        <  1:583195/100‑1 (MQ=255)

                                                         tcacttcacAATTTGTTTACATCAATTTGAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGAGGCCTCTATGGc                                      <  2:1077850/100‑1 (MQ=255)

                                                           acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                    >  1:2542765/1‑100 (MQ=255)

                                                           acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                    <  1:2437329/100‑1 (MQ=255)

                                                           acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                    <  2:1294823/100‑1 (MQ=255)

                                                           acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                    <  1:1550693/100‑1 (MQ=255)

                                                            cttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctct                                   >  2:1678000/1‑100 (MQ=255)

                                                            cttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctct                                   >  1:1882812/1‑100 (MQ=255)

                                                             ttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGACCGTAAAGATTCATGCCTCGCCTGCGTGGCCTCTATGGctcag                                  <  1:1732057/100‑3 (MQ=255)

                                                               cacaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                                >  2:2330486/1‑100 (MQ=255)

                                                               cacaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                                <  1:2210298/100‑1 (MQ=255)

                                                               cacaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                                <  1:380197/100‑1 (MQ=255)

                                                                acaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAtt                               <  1:1191848/100‑1 (MQ=255)

                                                                acaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAtt                               <  1:2161353/100‑1 (MQ=255)

                                                                 caATTTGTTTACATCGAGTTTAATACATGGTTAACGATCTGCCTGTATACAGCTCGTATAGATTGATGCCTCGCCTGCGTGGTCCCCATGGCTCTGa                                 <  2:630022/97‑1 (MQ=255)

                                                                 caATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAttt                              >  2:2183939/1‑100 (MQ=255)

                                                                 caATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAttt                              >  1:769235/1‑100 (MQ=255)

                                                                 caATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAttt                              >  2:1164597/1‑100 (MQ=255)

                                                                  aaTTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTa                             <  1:2000166/100‑1 (MQ=255)

                                                                    tttGTTTACATCAATTTTAACACATCATTAACAATCCGTCTGTATACAGTTCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGTTCTGATTTAAg                           <  2:1966535/100‑1 (MQ=255)

                                                                     ttGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGt                          <  1:1678012/100‑1 (MQ=255)

                                                                      tGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTa                         >  1:697125/1‑100 (MQ=255)

                                                                      tGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTa                         <  1:509822/100‑1 (MQ=255)

                                                                        tttACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAat                       >  1:415582/1‑100 (MQ=255)

                                                                        tttACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAat                       <  1:442934/100‑1 (MQ=255)

                                                                        tttACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAat                       <  2:2542749/100‑1 (MQ=255)

                                                                         ttACCTTAAGTTGAACGCATCATTACCAATCCGTCTGTATAGCGATCGTAAAGATTGATGAGTCGCTTGCGTGGCCTCTATGGCTCTGATTTAAg                           <  2:2259230/95‑1 (MQ=255)

                                                                            cATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                   >  1:1430546/1‑100 (MQ=255)

                                                                             aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg                  >  2:1285296/1‑100 (MQ=255)

                                                                             aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg                  >  2:1285419/1‑100 (MQ=255)

                                                                             aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAAATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGCTTTAAGTAATATCTg                  >  2:2226295/1‑100 (MQ=255)

                                                                                 aTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCggg              >  1:625528/1‑100 (MQ=255)

                                                                                 aTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCggg              <  1:1581817/100‑1 (MQ=255)

                                                                                  ttttAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGc             >  2:1916058/1‑100 (MQ=255)

                                                                                    ttAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGt           <  1:822501/100‑1 (MQ=255)

                                                                                     tAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGc             >  2:1459328/1‑97 (MQ=255)

                                                                                     tAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTg          >  2:893621/1‑100 (MQ=255)

                                                                                        cacaTCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTc       >  1:1399221/1‑100 (MQ=255)

                                                                                         acaTCATTTACATTCCGTCTTTATACAGATCTTAAATATTTATGCCTTGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTgg         <  2:2381617/97‑1 (MQ=255)

                                                                                          catcatTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTGAAGTAATATCTGCGAGCGTGGTCAc     >  2:2286498/1‑100 (MQ=255)

                                                                                           atcattAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACg    <  1:1645762/100‑1 (MQ=255)

                                                                                            tcattaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGt   <  1:680741/100‑1 (MQ=255)

                                                                                             cattaACAATCCGTCTTTATACAGATCGTAACGATTGATACCACGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGtt  >  2:837404/1‑100 (MQ=255)

                                                                                             cattaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGtt  >  1:647264/1‑100 (MQ=255)

CAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTT  >  NC_000913/2278202‑2278394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A