Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F133 I0 R1	200	171.4	8351354	94.3%	7875326	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,278,298	C→T	100%	intergenic (‑61/+272)	yejG ← / ← bcr	uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,278,298	0	C	T	98.8%	378.8 / ‑4.3	119	intergenic (‑61/+272)	yejG/bcr	uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (51/66); total (52/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

CGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTT  >  NC_000913/2278206‑2278394

cGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATc                                                                                           >  2:145974/1‑100 (MQ=255)

cGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATc                                                                                           <  1:782821/100‑1 (MQ=255)

 gATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTCCATCAATTTTAACACATCATTAACAATc                                                                                           >  2:3748522/1‑99 (MQ=255)

  aTGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCg                                                                                         <  2:1100896/100‑1 (MQ=255)

     ggcGATTGAGAGTTGTAATAAAGTCAATTATTTTCCTCAGAAGCACCTTTACTACAAACTTTGTTTGCATCAGTTCTAACACATCATTCACAATcc                                                                                          >  2:1500933/3‑96 (MQ=255)

     gACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCt                                                                                      >  1:1413590/1‑100 (MQ=255)

     gACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCt                                                                                      >  2:3898356/1‑100 (MQ=255)

     gACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCt                                                                                      <  2:702645/100‑1 (MQ=255)

       cGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCttt                                                                                    <  2:626562/100‑1 (MQ=255)

        gATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTta                                                                                   <  1:1725359/100‑1 (MQ=255)

         aTTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTtat                                                                                  >  2:1121411/1‑100 (MQ=255)

         aTTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTtat                                                                                  >  2:848021/1‑100 (MQ=255)

          ttGAGAGCTGTAATGAAGTCCGTTATTTTCTCCGGAAGCACATTTACTACACAATTTGTTTACATCAATCTTAACACATCATTAACAATCTGACTTtat                                                                                  >  2:4050671/1‑99 (MQ=255)

           tGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATAc                                                                                <  2:2384816/100‑1 (MQ=255)

           tGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATAc                                                                                >  1:3310012/1‑100 (MQ=255)

            gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCgagatcggaa                                                                                >  1:3747490/1‑90 (MQ=255)

            gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                               <  1:247944/100‑1 (MQ=255)

            gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                               <  2:751044/100‑1 (MQ=255)

            gagaGTTGTAAAGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACCTCATTAACAATCCGTCTTTATACa                                                                               <  2:1490964/100‑1 (MQ=255)

             agagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAg                                                                              <  2:1919555/100‑1 (MQ=255)

             agagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAg                                                                              <  2:1395069/100‑1 (MQ=255)

             agagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAg                                                                              <  2:469965/100‑1 (MQ=255)

              gagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTATTTATACAGa                                                                             >  2:4002592/1‑100 (MQ=255)

                gTTGTAATGAAGTCACTTATTTTCCCCTGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATc                                                                           <  2:834574/100‑1 (MQ=255)

                gTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATc                                                                           <  2:2605554/100‑1 (MQ=255)

                gTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATc                                                                           <  1:2241640/100‑1 (MQ=255)

                  tGTAATGAAGTCACTTATTTTCCCCGGATGTATATTCACTTCACAATTTGTTTACATCAATTTTAACACACCATTAACAATCCGTCTTTATACAg                                                                              <  2:2160628/95‑1 (MQ=255)

                   gTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATGAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTa                                                                        >  2:2669920/1‑100 (MQ=255)

                   gTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTa                                                                        >  2:1185287/1‑100 (MQ=255)

                   gTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTa                                                                        >  2:4122288/1‑100 (MQ=255)

                    tAATGAAGTCACTTATTTTCCCCGGAATCACATTCACTTCACAATTTGTTTACATCAATTTTAAGACATCATTAACAATCCGTCTTTATACAGATCGTa                                                                        <  2:3519963/99‑1 (MQ=255)

                    tAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTaa                                                                       >  2:3764913/1‑100 (MQ=255)

                        gAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAt                                                                   >  2:1836798/1‑100 (MQ=255)

                         aaGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAtt                                                                  <  2:2156597/100‑1 (MQ=255)

                         aaGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAtt                                                                  >  2:1837802/1‑100 (MQ=255)

                         aaGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAtt                                                                  <  1:3064774/100‑1 (MQ=255)

                          aGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTg                                                                 <  1:3394994/100‑1 (MQ=255)

                           gacACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGa                                                                >  1:2804551/3‑100 (MQ=255)

                           gTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGa                                                                >  1:2508906/1‑100 (MQ=255)

                             cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGAt                                                               >  2:3927202/1‑99 (MQ=255)

                             cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              <  2:3663584/100‑1 (MQ=255)

                             cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              >  2:1726461/1‑100 (MQ=255)

                             cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              >  2:1087065/1‑100 (MQ=255)

                             cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              <  1:3737661/100‑1 (MQ=255)

                              aCTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGc                                                             >  1:237497/1‑100 (MQ=255)

                              aCTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGc                                                             <  1:1849988/100‑1 (MQ=255)

                               cTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGcc                                                            <  2:371852/100‑1 (MQ=255)

                               cTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTAGACAGATCGTAAAGATTGATGcc                                                            <  2:2644194/100‑1 (MQ=255)

                                 tATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTc                                                          >  1:651260/1‑100 (MQ=255)

                                  aTTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCg                                                         >  2:3407970/1‑100 (MQ=255)

                                  aTTTTCCCCGGAAGAACATTCAATTCACAATTTGTTTCCATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCg                                                         <  2:3478483/100‑1 (MQ=255)

                                    tttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGcc                                                       >  2:1776547/1‑100 (MQ=255)

                                           ggAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTgg                                                <  1:3259920/100‑1 (MQ=255)

                                           ggAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTg                                                 <  2:378826/99‑1 (MQ=255)

                                            gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGc                                               <  1:1511545/100‑1 (MQ=255)

                                              ngCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCct                                             >  2:307906/2‑100 (MQ=255)

                                              aGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCct                                             >  1:1635154/1‑100 (MQ=255)

                                              aGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCct                                             >  2:1805588/1‑100 (MQ=255)

                                              aGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCct                                             <  1:3895725/100‑1 (MQ=255)

                                               gCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctc                                            <  1:295714/100‑1 (MQ=255)

                                               gCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctc                                            <  1:60459/100‑1 (MQ=255)

                                                cacaTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctct                                           >  1:2631099/1‑100 (MQ=255)

                                                cacaTGCACTTCACAATTTGTTTACATCAATGTTAACACAGCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGAGGCCTCGCCTGCGTGGCctct                                           <  2:2797515/100‑1 (MQ=255)

                                                 acaTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTa                                          <  1:706981/100‑1 (MQ=255)

                                                  caTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTAt                                         <  1:1620045/100‑1 (MQ=255)

                                                   aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                        <  2:322126/100‑1 (MQ=255)

                                                    ttcacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTTGCCTCTATgg                                       >  2:1167126/1‑100 (MQ=255)

                                                     tcacttcacAATTTGTTTACATCAATTTTAACACATCTTTAACAACCCGTCTTTATACTGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATgg                                       <  1:372624/99‑1 (MQ=255)

                                                      cacttcagAATTTGTTTACATCAATGTTAACACATCAGTAACAATCCGTCTTGATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGct                                     <  1:4171192/100‑1 (MQ=255)

                                                      cacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGct                                     >  1:3909171/1‑100 (MQ=255)

                                                      cacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGct                                     >  1:897700/1‑100 (MQ=255)

                                                      cacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGc                                      <  1:1696132/99‑1 (MQ=255)

                                                       acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                    >  1:3210288/1‑100 (MQ=255)

                                                        cttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctct                                   >  1:2594436/1‑100 (MQ=255)

                                                        cttcacAATTTGTTTACATCAATTTTAACACATCATTAACAAGCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                    <  1:1922694/99‑1 (MQ=255)

                                                           cacaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                                <  1:970997/100‑1 (MQ=255)

                                                              aaTTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTa                             <  1:874807/100‑1 (MQ=255)

                                                              aaTTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTa                             >  1:3565598/1‑100 (MQ=255)

                                                              aaTTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTa                             <  1:2985582/100‑1 (MQ=255)

                                                                tttGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAg                           <  2:322153/100‑1 (MQ=255)

                                                                   gTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTTCGTGGCCTCTATGGCTCTGATTTAAGTaa                        >  2:3664989/1‑100 (MQ=255)

                                                                     ttgcATCAATTTTAACACATCATTAACAATCCGTATTTATACAGATCGTAAACATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTaaga                      <  2:1337061/97‑3 (MQ=255)

                                                                     ttACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAata                      >  2:4104984/1‑100 (MQ=255)

                                                                        cATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                   >  2:2004397/1‑100 (MQ=255)

                                                                        cATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                   >  2:981654/1‑100 (MQ=255)

                                                                        cATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                   >  2:981655/1‑100 (MQ=255)

                                                                         aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg                  >  1:789120/1‑100 (MQ=255)

                                                                          tCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGc                 >  2:2714922/1‑100 (MQ=255)

                                                                          tCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGc                 >  2:574130/1‑100 (MQ=255)

                                                                           cAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCg                >  2:545602/1‑100 (MQ=255)

                                                                           cAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCg                <  2:2330814/100‑1 (MQ=255)

                                                                             aTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCggg              <  1:689108/100‑1 (MQ=255)

                                                                              ttttAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGc             >  2:1780015/1‑100 (MQ=255)

                                                                               tttAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCg            <  2:2396739/100‑1 (MQ=255)

                                                                                 tAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCgac          <  1:1152934/100‑3 (MQ=255)

                                                                                  aaCACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCTTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTgg         <  2:2307783/100‑1 (MQ=255)

                                                                                   acacATCATTAACAATCCTTCTTTAGACAGATCGTAAAGATTGATGCCTCACCTGCGTGCCCTCTAGGGCGCTGCTTTAAGTCATATCTGCg                >  2:3322884/1‑92 (MQ=255)

                                                                                     acaTCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCa      <  1:2713216/100‑1 (MQ=255)

                                                                                      catcatTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCAc     >  2:1745954/1‑100 (MQ=255)

                                                                                         cattaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGCGGCCTCTATGGCTATGATTTAAGTAATATCTGCGGGGGTGGTCACGtt  >  2:4125484/1‑100 (MQ=255)

CGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTT  >  NC_000913/2278206‑2278394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A