Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F142 I1 R1
|
99 |
64.8 |
3148104 |
96.3% |
3031624 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,490,055 |
T→G |
E3D (GAA→GAC) |
yjgR ← |
DUF853 family protein with NTPase fold |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,490,055 | 0 | T | G | 97.4%
| 134.5
/ ‑3.4
| 38 | E3D (GAA→GAC) | yjgR | DUF853 family protein with NTPase fold |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (17/20); total (17/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 |
CCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTG > NC_000913/4489961‑4490150
|
ccGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACt > 2:1496303/1‑100 (MQ=255)
ccGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACt < 1:518746/100‑1 (MQ=255)
cGTCCCCTTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTc < 1:427208/100‑1 (MQ=255)
cGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTc < 2:1461096/100‑1 (MQ=255)
gTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCa < 1:932150/100‑1 (MQ=255)
tCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCAt < 1:1413391/100‑1 (MQ=255)
ccccGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATg < 2:1539169/100‑1 (MQ=255)
cccGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATga > 1:1221944/1‑100 (MQ=255)
cgcCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcc > 1:727357/1‑100 (MQ=255)
cGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcctcc > 2:1467596/1‑100 (MQ=255)
aaTCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGtt < 1:368274/100‑1 (MQ=255)
aaTCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGtt < 1:382513/100‑1 (MQ=255)
aTCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGttt > 1:1129498/1‑100 (MQ=255)
aTCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGttt < 1:953078/100‑1 (MQ=255)
cAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcc > 2:628430/1‑100 (MQ=255)
aTGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgcctg < 1:605136/100‑1 (MQ=255)
gACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTgg < 1:384746/99‑1 (MQ=255)
aCGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgcctg < 1:430316/97‑1 (MQ=255)
ttAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGtata < 1:1259705/100‑1 (MQ=255)
aGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgg < 2:156635/100‑1 (MQ=255)
aGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgg > 2:451280/1‑100 (MQ=255)
gCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGc > 1:862765/1‑100 (MQ=255)
cATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCaa < 2:378104/100‑1 (MQ=255)
cATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTACGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGtat > 2:649126/1‑94 (MQ=255)
ttCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACg < 1:440788/100‑1 (MQ=255)
ttCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACg < 1:1447393/100‑1 (MQ=255)
gTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCaaa > 1:33284/1‑100 (MQ=255)
aaaaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAaga > 2:472589/1‑100 (MQ=255)
aaaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAagag > 2:1140011/1‑100 (MQ=255)
aaaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAagag > 1:1420347/1‑100 (MQ=255)
aaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAgaga > 1:1478032/1‑100 (MQ=255)
gTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACg < 2:906967/100‑1 (MQ=255)
gTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACg > 1:801591/1‑100 (MQ=255)
tgtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAAt < 2:1451201/100‑1 (MQ=255)
tgtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAAt < 2:1223337/100‑1 (MQ=255)
cGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAag < 1:1467605/100‑1 (MQ=255)
aaCAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAg > 2:138904/1‑100 (MQ=255)
ggggGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCgtg > 1:1044606/1‑100 (MQ=255)
|
CCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTG > NC_000913/4489961‑4490150
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
