Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,490,055 T→G 100% E3D (GAA→GAC)  yjgR ← DUF853 family protein with NTPase fold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,490,0550TG98.7% 532.9 / ‑2.7 152E3D (GAA→GAC) yjgRDUF853 family protein with NTPase fold
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (77/73);  total (77/75)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 154 total aligned reads displayed.

ACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGT  >  NC_000913/4489959‑4490151
                                                                                                |                                                                                                
aCCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCa                                                                                               >  1:3566150/1‑100 (MQ=255)
 cccGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCAc                                                                                              >  2:2154832/1‑100 (MQ=255)
  ccGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACt                                                                                             <  2:2319675/100‑1 (MQ=255)
   cGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTc                                                                                            >  1:1015504/1‑100 (MQ=255)
    gTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCa                                                                                           <  1:3033073/100‑1 (MQ=255)
    gTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCa                                                                                           <  2:1054451/100‑1 (MQ=255)
       cccGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATga                                                                                        <  2:3703795/100‑1 (MQ=255)
        ccGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATgag                                                                                       <  1:2680186/100‑1 (MQ=255)
         cGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATgaga                                                                                      >  1:3833278/1‑100 (MQ=255)
          gTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGat                                                                                     >  2:2860673/1‑100 (MQ=255)
           ttGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGata                                                                                    <  2:3545434/100‑1 (MQ=255)
              cgcCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcc                                                                                 <  2:75070/100‑1 (MQ=255)
               gcCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcct                                                                                <  1:1010296/100‑1 (MQ=255)
               gcCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcct                                                                                >  1:3520994/1‑100 (MQ=255)
                ccGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcctc                                                                               >  1:1722299/1‑100 (MQ=255)
                ccGGCAATCCGCCCATGACGGTTAGCCATACCAAAAAAAAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcct                                                                                <  2:3093999/99‑1 (MQ=255)
                  ggTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCa                                                                             >  2:4098001/1‑100 (MQ=255)
                  ggTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCa                                                                             >  1:1985480/1‑100 (MQ=255)
                  ggTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCATCCa                                                                             >  2:3848149/1‑100 (MQ=255)
                  ggTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGGGCGGGCAATTAACCGGGGGGCACTCATGAGATATCCTCCa                                                                             >  1:281808/1‑100 (MQ=255)
                   gTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAg                                                                            <  1:3451050/100‑1 (MQ=255)
                     aaTCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGtt                                                                          <  1:851731/100‑1 (MQ=255)
                      aTCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGttt                                                                         <  2:34730/100‑1 (MQ=255)
                       taaGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTc                                                                        <  2:104336/98‑1 (MQ=255)
                       tCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTc                                                                        <  2:943591/100‑1 (MQ=255)
                          gCCCATGACGGTTAGCCATTCCCTGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctg                                                                     >  2:1229417/1‑100 (MQ=255)
                          gCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctg                                                                     >  1:1750298/1‑100 (MQ=255)
                             cATGAGGGTTAGGCATTCCCGGCAGTAAAAACAGTTCTGGGGCGGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATTCTCCAGTTTcctgcct                                                                  <  2:870956/100‑1 (MQ=255)
                             cATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgcct                                                                  <  2:3696149/100‑1 (MQ=255)
                             cATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgcct                                                                  <  2:1840614/100‑1 (MQ=255)
                             cATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgcct                                                                  >  1:3700339/1‑100 (MQ=255)
                              aTGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgcctg                                                                 <  1:1591755/100‑1 (MQ=255)
                               tGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTgg                                                                >  1:819109/1‑100 (MQ=255)
                               tGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCACTTTactgcctg                                                                 >  1:3903166/1‑99 (MQ=255)
                                 aCGGTTAGCCATTCCCGGCAGTAAAAAGAGTTCTCTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAg                                                              <  2:2195135/100‑1 (MQ=255)
                                 aCGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAg                                                              <  2:1741793/100‑1 (MQ=255)
                                 aCGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAg                                                              <  1:65212/100‑1 (MQ=255)
                                  cGGTTCGCCATTCCCGTCAGTAAAAACAGTTCTGTTTCCGGCGTGCGGGCAATTAACTGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGt                                                             <  1:2656991/100‑1 (MQ=255)
                                   ggTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGta                                                            >  1:2306206/1‑100 (MQ=255)
                                    gTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGtat                                                           <  2:3944301/100‑1 (MQ=255)
                                       aGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgg                                                        <  1:1075475/100‑1 (MQ=255)
                                        gCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGc                                                       <  1:1650833/100‑1 (MQ=255)
                                        gCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGc                                                       >  2:698829/1‑100 (MQ=255)
                                        gCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGc                                                       >  1:880418/1‑100 (MQ=255)
                                         ccATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCa                                                      >  2:4020489/1‑100 (MQ=255)
                                          cATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCaa                                                     <  1:4004331/100‑1 (MQ=255)
                                           aTTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGGGTATAGGCAAc                                                    >  2:3151804/1‑100 (MQ=255)
                                             tCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGt                                                  >  2:3296981/1‑100 (MQ=255)
                                              cccGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTc                                                 >  1:1440610/1‑100 (MQ=255)
                                              cccGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTc                                                 >  1:3346403/1‑100 (MQ=255)
                                                 ggCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTCACAGGGGGTTACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCgg                                              <  2:298871/100‑1 (MQ=255)
                                                  gCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGc                                             <  1:3204551/100‑1 (MQ=255)
                                                  gCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGAGGGTCACTCATGCGATCTCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCAgg                                              >  2:2915848/1‑99 (MQ=255)
                                                   cAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGc                                             <  2:1203307/99‑1 (MQ=255)
                                                   cAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCa                                            <  1:3191145/100‑1 (MQ=255)
                                                    aGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCaa                                           >  1:3511779/1‑100 (MQ=255)
                                                     gTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCaaa                                          >  2:3100424/1‑100 (MQ=255)
                                                     gTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCaaa                                          >  1:3185140/1‑100 (MQ=255)
                                                      tAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAag                                         <  1:2867194/100‑1 (MQ=255)
                                                      tAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAag                                         >  1:353585/1‑100 (MQ=255)
                                                       aaaaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAaga                                        <  2:3630809/100‑1 (MQ=255)
                                                       aaaaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGAGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAaga                                        <  1:2041337/100‑1 (MQ=255)
                                                         aaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCGGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAgaga                                      <  2:2512689/100‑1 (MQ=255)
                                                         aaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAgaga                                      >  1:927278/1‑100 (MQ=255)
                                                           aCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAAt                                    <  2:1916038/100‑1 (MQ=255)
                                                           aCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAAt                                    <  1:1549750/100‑1 (MQ=255)
                                                            cAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATa                                   <  2:1570877/100‑1 (MQ=255)
                                                            cAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATa                                   >  2:33996/1‑100 (MQ=255)
                                                              gTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACg                                 >  1:2161834/1‑100 (MQ=255)
                                                              gTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACg                                 >  1:2562926/1‑100 (MQ=255)
                                                               ttaaatgtCCGGCGTGCGGGCAATTCACAGGAGGTCCCTCACAAGATCTCCTCAAGTTTACTATTTAGTGTCTAGGCAACGGCCGGCAAAGCGAATAc                                  <  2:344585/93‑1 (MQ=255)
                                                                tCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACgtg                               >  1:3436523/1‑100 (MQ=255)
                                                                 cTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATGCTCCAGTATCCTGCCAGGAGTATAGGCAACGTCCGGCAAAGAGAATACg                                 >  2:2463567/1‑97 (MQ=255)
                                                                   gtgtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTaa                            >  1:1219030/1‑100 (MQ=255)
                                                                    tgtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAAt                           <  2:3286334/100‑1 (MQ=255)
                                                                     gtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATg                          >  2:3132130/1‑100 (MQ=255)
                                                                     gtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATg                          <  2:675324/100‑1 (MQ=255)
                                                                     gtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGACTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATg                          >  2:2296508/1‑100 (MQ=255)
                                                                      tCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATg                          <  1:2488824/99‑1 (MQ=255)
                                                                        cGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAag                       >  1:1142217/1‑100 (MQ=255)
                                                                        cGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAag                       <  2:3986542/100‑1 (MQ=255)
                                                                         ggCGTGCGGGCAATTAACAGGGAGTCACTCATGAGATATCCTCCAGTCTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAaga                      <  2:2812994/100‑1 (MQ=255)
                                                                             tGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGaaa                  >  1:124827/1‑100 (MQ=255)
                                                                              gCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGaaaa                 <  2:2172517/100‑1 (MQ=255)
                                                                               cGGGGAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAgaga                    <  2:3237269/96‑1 (MQ=255)
                                                                               cGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAAt                <  2:2005340/100‑1 (MQ=255)
                                                                               cGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAAt                >  2:1794179/1‑100 (MQ=255)
                                                                                gggCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTACTGCCTGGAGTATAGGGAACGTCCGGCAAAGAGAATACGTGTAATGAGGAGAAAAt                >  2:384538/1‑99 (MQ=255)
                                                                                  gCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAAc             <  1:1629815/100‑1 (MQ=255)
                                                                                  gCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAAc             <  2:64331/100‑1 (MQ=255)
                                                                                  gCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAAc             <  1:593875/100‑1 (MQ=255)
                                                                                   cAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAAAGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAAcc            >  2:484446/1‑100 (MQ=255)
                                                                                    aaTTAACAGGGGGTCTCTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCt           <  1:4117845/100‑1 (MQ=255)
                                                                                      ttAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTaa         >  1:2210401/1‑100 (MQ=255)
                                                                                      ttAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTaa         >  2:1564641/1‑100 (MQ=255)
                                                                                      ttAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTaa         >  2:1871309/1‑100 (MQ=255)
                                                                                       tAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTaaa        >  2:3414916/1‑100 (MQ=255)
                                                                                            ggggGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCgtg   >  2:593002/1‑100 (MQ=255)
                                                                                            ggggGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCgtg   <  2:1163591/100‑1 (MQ=255)
                                                                                             ggggTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCgtgt  >  1:2689092/1‑100 (MQ=255)
                                                                                                |                                                                                                
ACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGT  >  NC_000913/4489959‑4490151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A