Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F133 I1 R1
|
102 |
83.9 |
4092546 |
96.1% |
3932936 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,490,055 |
T→G |
E3D (GAA→GAC) |
yjgR ← |
DUF853 family protein with NTPase fold |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,490,055 | 0 | T | G | 97.0%
| 233.3
/ ‑2.8
| 66 | E3D (GAA→GAC) | yjgR | DUF853 family protein with NTPase fold |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (33/31); total (34/32) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 |
TACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTG > NC_000913/4489958‑4490150
|
tACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTc < 1:1532888/100‑1 (MQ=255)
aCCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCa > 2:1617389/1‑100 (MQ=255)
cccGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCAc > 1:875047/1‑100 (MQ=255)
cccGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCAc < 2:1017525/100‑1 (MQ=255)
cccGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGACAATTAACAGGGGGTCAc > 1:1275440/1‑100 (MQ=255)
cGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTc > 2:1085419/1‑100 (MQ=255)
ccccGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATg < 1:1043121/100‑1 (MQ=255)
ccccGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATg < 1:116644/100‑1 (MQ=255)
ccGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATgag < 2:6342/100‑1 (MQ=255)
gTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGat < 1:1729748/100‑1 (MQ=255)
tGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGatat < 2:770521/100‑1 (MQ=255)
tGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGatat < 2:36982/100‑1 (MQ=255)
gcCGGTAATCAGAGCATGGCGGTTAGAACTTCCAGGCTGTACACACCGTTCTGTGTGCGGCGTGCGGGCAATGAACATTACTGCACTCAGGATATAtcc < 2:1907958/99‑1 (MQ=255)
cGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcctcc < 2:767952/100‑1 (MQ=255)
tAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGt < 2:1765439/100‑1 (MQ=255)
aaTCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGtt > 2:1881717/1‑100 (MQ=255)
aTCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGttt > 2:181626/1‑100 (MQ=255)
tCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTc > 2:758931/1‑100 (MQ=255)
cAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcc < 2:1791571/100‑1 (MQ=255)
cAGCCCATGACAGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcc < 2:426868/100‑1 (MQ=255)
gCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctg > 1:1192343/1‑100 (MQ=255)
gCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctg > 2:1295486/1‑100 (MQ=255)
gCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctg > 2:11982/1‑100 (MQ=255)
cccATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgc > 2:637921/1‑100 (MQ=255)
aTGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgcc < 2:828438/98‑1 (MQ=255)
aCGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAg < 1:1617402/100‑1 (MQ=255)
aCGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAg < 1:1456891/100‑1 (MQ=255)
ggTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGta < 1:132753/100‑1 (MQ=255)
aGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgg > 1:707183/1‑100 (MQ=255)
aGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgg < 1:1445198/100‑1 (MQ=255)
aGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgg < 2:1227105/100‑1 (MQ=255)
ttCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAAGTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACg < 2:1433713/100‑1 (MQ=255)
cccGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTc > 1:530510/1‑100 (MQ=255)
cccGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTc > 1:1540207/1‑100 (MQ=255)
cGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCg < 1:1922338/100‑1 (MQ=255)
cGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATCAGATATCCTCCAGTTTCCTGCCTGGAGTTTAGGCAACGTCCg < 1:1382128/100‑1 (MQ=255)
ggCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCgg > 2:181948/1‑100 (MQ=255)
gCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGc > 1:1835347/1‑100 (MQ=255)
gCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGACAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGc < 2:1275434/100‑1 (MQ=255)
cAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCa < 1:1101894/100‑1 (MQ=255)
ggaaaatCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTGATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCaaa > 2:601961/3‑100 (MQ=255)
aaaaaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCaca > 2:131625/2‑97 (MQ=255)
aCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAAt < 1:1743667/100‑1 (MQ=255)
gTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACg > 1:165298/1‑100 (MQ=255)
gtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATg < 2:384207/100‑1 (MQ=255)
gtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATg > 2:1335094/1‑100 (MQ=255)
gtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATg > 1:794869/1‑100 (MQ=255)
ccGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGaa > 1:381780/1‑100 (MQ=255)
cGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAag < 1:1295493/100‑1 (MQ=255)
cGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAag > 1:986981/1‑100 (MQ=255)
cGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAag < 1:894510/100‑1 (MQ=255)
ggcgggcgGTGAATTAACAGGGGGTCACGCATGAGATAGCCTCCAGTTTCCTGCCTGTAGAATAGGCAACGTCCGGCAAAGTGAAGACGTGTAATGAag < 2:1763403/99‑1 (MQ=255)
gCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAagag > 2:1759303/1‑100 (MQ=255)
gTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGaa < 1:1335103/100‑1 (MQ=255)
cGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAAt > 2:1393667/1‑100 (MQ=255)
cGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAAt < 1:476481/100‑1 (MQ=255)
gggCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATa < 1:1085424/100‑1 (MQ=255)
ggCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATaa > 1:354564/1‑100 (MQ=255)
ggCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATaa > 1:672134/1‑100 (MQ=255)
ggCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATaa > 1:570041/1‑100 (MQ=255)
cAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAAcc > 2:1066787/1‑100 (MQ=255)
aaCAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAg > 2:2036201/1‑100 (MQ=255)
aaCAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAg > 1:1617114/1‑100 (MQ=255)
aaCAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAg > 2:798076/1‑100 (MQ=255)
cAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCg > 1:1404745/1‑100 (MQ=255)
ggggGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCgtg > 2:210205/1‑100 (MQ=255)
|
TACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTG > NC_000913/4489958‑4490150
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
