Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F142 I0 R1
|
175 |
91.2 |
4812830 |
94.5% |
4548124 |
99.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,827,031 |
T→A |
100% |
D410V (GAT→GTT) |
astB ← |
succinylarginine dihydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,827,031 | 0 | T | A | 93.8%
| 234.2
/ 3.8
| 77 | D410V (GAT→GTT) | astB | succinylarginine dihydrolase |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base A (33/38); total (36/41) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
CGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGG > NC_000913/1826936‑1827129
|
cgcgCTGGAACGGATAAGCCGAACCGAGATTCAGTAATTGGCTAAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgc < 1:1114571/100‑1 (MQ=255)
cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgc < 2:1833916/100‑1 (MQ=255)
cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgc < 2:642797/100‑1 (MQ=255)
cgcTGGAACGGATAACCCGAACCGAGATTCAGTACTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgca < 2:2396446/99‑1 (MQ=255)
tGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGcagcag < 1:1069398/100‑1 (MQ=255)
ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGt > 1:1799764/1‑100 (MQ=255)
aaCGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCGGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTaa > 2:1712754/1‑100 (MQ=255)
aaCGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTGCCACACATGGCGAAGCAAATGAGGGTCGGACAGAACg > 2:1516289/1‑90 (MQ=255)
aaCGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCCGCAGTa > 1:1007749/1‑99 (MQ=255)
cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAgg < 2:2277095/100‑1 (MQ=255)
ggATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGc > 1:1967704/1‑100 (MQ=255)
gATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGc > 1:252757/1‑99 (MQ=255)
gATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCg > 1:453212/1‑100 (MQ=255)
aTAAACCGAACCGAGAGTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGa < 1:921163/100‑1 (MQ=255)
aaaCCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATc > 1:1900329/1‑100 (MQ=255)
aaaCCGAACCGAGATTCAGTAATTCGCTCAATACCGCCAGAGCTTCCCGCCCTTCGCACAGCAATTGCGGGTCGGACAGCACGGCAGCAGTAACGcca > 2:1289426/1‑96 (MQ=255)
aaccgaaccaAGATCCAATAATTGAGTCAGTGTATCCAGCGCTTCCCTTCCTTCGCGCAGCAATTGTGGATCGGCAAGATCCGCCGCAGTAAGACGATcg > 2:730946/1‑100 (MQ=255)
ccgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcgcg > 1:642418/1‑100 (MQ=255)
cgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCgg < 1:1470192/100‑1 (MQ=255)
gaagcgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGGCGCAATTGCGGGTCGGACAGAACTGCTGCAGTAAGGCGATc > 2:1787627/1‑95 (MQ=255)
gaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgt < 1:2122501/100‑1 (MQ=255)
ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATTGCGgtagt > 1:409361/1‑100 (MQ=255)
ccgaGATTCAGTAATTGGATCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGACAGCAGTAAGGCGATCGCGgtagt > 2:376328/1‑100 (MQ=255)
gagaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagta < 1:89660/99‑1 (MQ=255)
ttCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGAt < 2:65010/100‑1 (MQ=255)
ttCAGTAATTGGCTCAATACATCCAGTGCGTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGAt < 2:1190405/100‑1 (MQ=255)
tCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATc < 1:2392759/100‑1 (MQ=255)
tCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATc < 1:2315481/100‑1 (MQ=255)
cAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATcc > 2:83383/1‑100 (MQ=255)
aGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCa > 1:1983657/1‑100 (MQ=255)
aGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCa > 1:664199/1‑100 (MQ=255)
aGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCAGTAATAACGATcaa > 1:746047/1‑98 (MQ=255)
tAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATTCAcc < 1:126810/100‑1 (MQ=255)
aaTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAccc > 2:1556870/1‑100 (MQ=255)
aTTGGCTCAATACATCCAGTACTTCCCCCCCTTCGCGCAGCACTTGCGGGTCGGCCAGAACGGCAGCAGCACAGCGATCGCGGTTGTAACGATCCAc > 2:740658/1‑97 (MQ=255)
ttGGCTCAATACTTCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAg < 1:119023/100‑1 (MQ=255)
tGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGt > 2:401754/1‑100 (MQ=255)
cTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAt > 1:2211684/1‑100 (MQ=255)
cTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAt < 2:278067/100‑1 (MQ=255)
tCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAt < 1:52509/99‑1 (MQ=255)
tCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAt < 2:885774/99‑1 (MQ=255)
cAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTg < 1:2288506/100‑1 (MQ=255)
aaTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGa > 1:1723346/1‑100 (MQ=255)
aTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAg < 1:2104212/100‑1 (MQ=255)
tACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgc > 2:1239050/1‑100 (MQ=255)
aCATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcg < 2:944140/100‑1 (MQ=255)
aCATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcg < 1:249326/100‑1 (MQ=255)
cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcgc > 1:1807026/1‑100 (MQ=255)
cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgc > 1:615078/1‑98 (MQ=255)
tCCAGTGCTTCCCGCCCTTCGCGCAGAAATTGCGGGTCGGCCAGACCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAt > 2:872451/1‑100 (MQ=255)
ccAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAtt > 1:2279375/1‑100 (MQ=255)
ccAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAtt < 2:1431799/100‑1 (MQ=255)
ccAGCGCTTCCCTTCCTTCGCGCAGCAATTGTGGATCGGCAAGATCCGCCGCAGTAAGACGATAGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAtt < 2:2180893/100‑1 (MQ=255)
cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa > 1:800058/1‑100 (MQ=255)
tGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAAc < 1:2100489/100‑1 (MQ=255)
ttCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGc < 2:1007738/100‑1 (MQ=255)
tCCCGTCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg < 1:801679/100‑1 (MQ=255)
tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg < 1:868812/100‑1 (MQ=255)
ccGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTa > 2:1799244/1‑100 (MQ=255)
cccTTCGCGCAGGAATTGCGGTTCGTCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCTGATTAAACAGCGTATCg < 2:717186/100‑1 (MQ=255)
ccTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGt < 1:627504/100‑1 (MQ=255)
tCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtca < 2:1129502/100‑1 (MQ=255)
tCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtca < 1:545045/100‑1 (MQ=255)
gcgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatc < 1:1814560/100‑1 (MQ=255)
cgcAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca < 1:530757/100‑1 (MQ=255)
cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca < 2:274360/100‑1 (MQ=255)
ccgatctGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatcatca < 2:353347/94‑1 (MQ=255)
gcaATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAc > 2:2029596/1‑100 (MQ=255)
gcaATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAc < 1:1674370/100‑1 (MQ=255)
tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg < 2:1936659/100‑1 (MQ=255)
tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg > 1:28959/1‑100 (MQ=255)
gCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatcatcaagatcgg > 1:353350/1‑93 (MQ=255)
ggCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAcc < 1:1428630/100‑1 (MQ=255)
ggCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAc > 1:578812/1‑99 (MQ=255)
ccAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGc > 2:801022/1‑100 (MQ=255)
ccAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGACTCACCGc > 2:1627156/1‑100 (MQ=255)
aGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGcc < 2:1134362/99‑1 (MQ=255)
ctCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCgg < 2:998592/99‑1 (MQ=255)
|
CGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCCGG > NC_000913/1826936‑1827129
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
