Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F204 I0 R1	247	174.3	8556966	94.2%	8060661	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,827,031	T→A	100%	D410V (GAT→GTT)	astB ←	succinylarginine dihydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,827,031	0	T	A	98.6%	466.3 / ‑3.1	141	D410V (GAT→GTT)	astB	succinylarginine dihydrolase
Reads supporting (aligned to +/- strand): ref base T (0/2); new base A (75/63); total (75/66)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 141 total aligned reads displayed.

CTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCC  >  NC_000913/1826934‑1827127

ctcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCGCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACg                                                                                                >  2:3005890/1‑100 (MQ=255)

ctcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACg                                                                                                >  2:3462110/1‑100 (MQ=255)

  cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgc                                                                                              <  2:3487451/100‑1 (MQ=255)

  cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgc                                                                                              <  2:1137621/100‑1 (MQ=255)

  cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgc                                                                                              <  2:2056255/100‑1 (MQ=255)

    cgcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcag                                                                                            >  2:1879317/1‑100 (MQ=255)

    cgcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcag                                                                                            <  2:386101/100‑1 (MQ=255)

     gcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGTCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagc                                                                                           <  2:1074045/100‑1 (MQ=255)

     gcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGgcagc                                                                                           <  1:2075826/100‑1 (MQ=255)

      cTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagca                                                                                          >  1:2824435/1‑100 (MQ=255)

       tGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGcagcag                                                                                         <  2:131296/100‑1 (MQ=255)

       tGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGcagcag                                                                                         <  1:3752380/100‑1 (MQ=255)

        ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGcagcag                                                                                         >  2:2103173/1‑99 (MQ=255)

        ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGt                                                                                        >  1:3717254/1‑100 (MQ=255)

        ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGt                                                                                        >  2:3897491/1‑100 (MQ=255)

            cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAgg                                                                                    <  1:1204031/100‑1 (MQ=255)

            cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAgg                                                                                    <  1:632909/100‑1 (MQ=255)

             ggATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGc                                                                                   >  2:3682772/1‑100 (MQ=255)

              gATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCCGCAGCAAGGCg                                                                                  >  1:423693/1‑100 (MQ=255)

              gATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCg                                                                                  >  2:2044908/1‑100 (MQ=255)

                  aaccgaaccgAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcg                                                                              >  1:2663782/1‑100 (MQ=255)

                  aaccgaaccgAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcg                                                                              >  2:3495827/1‑100 (MQ=255)

                  aaccgaaccgAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcg                                                                              >  2:4225861/1‑100 (MQ=255)

                  aaccgaaccgAGATTCAGTAATTGGCTCAATACATCCAGTACTTCCCGCTCGTCGCGCAGCAATTGAGGGTGGGGCAGAACGGCAGCAGTAAGGCGATcg                                                                              >  1:1052310/1‑100 (MQ=255)

                   accgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcgc                                                                             >  1:1123270/1‑100 (MQ=255)

                    ncgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcgcg                                                                            >  2:3328742/2‑100 (MQ=255)

                     cgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCgg                                                                           <  2:1855016/100‑1 (MQ=255)

                     cgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCgg                                                                           <  2:2291069/100‑1 (MQ=255)

                     cgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCgg                                                                           <  1:1408000/100‑1 (MQ=255)

                      gaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgt                                                                          <  2:2406906/100‑1 (MQ=255)

                      gaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgt                                                                          <  1:1763185/100‑1 (MQ=255)

                         ccgaGATTCAGTAATTGGCTCAATGCATCCATTGCTTCCCGCCCTTCGCGCAGCAATTGCGGCCCGGCCAGAACGGCTGCAGTAAGGCGATcgc                                                                             >  1:2518907/1‑94 (MQ=255)

                         ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagt                                                                       >  2:1674364/1‑100 (MQ=255)

                         ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagt                                                                       >  2:4109435/1‑100 (MQ=255)

                         ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGCTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtag                                                                        >  1:604177/1‑99 (MQ=255)

                         ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTACGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagt                                                                       >  1:366634/1‑100 (MQ=255)

                         ccgaGATTCAGTAATTAGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGAACGCGgtagt                                                                       >  2:166884/1‑100 (MQ=255)

                           gagaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTaa                                                                     >  1:3072676/1‑100 (MQ=255)

                            agaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAAc                                                                    <  2:3180/100‑1 (MQ=255)

                             gaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACg                                                                   <  2:1128577/100‑1 (MQ=255)

                              aTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGa                                                                  >  2:3913783/1‑100 (MQ=255)

                                tCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATc                                                                <  1:198550/100‑1 (MQ=255)

                                 cAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATcc                                                               >  1:851094/1‑100 (MQ=255)

                                 cAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATcc                                                               >  2:529578/1‑100 (MQ=255)

                                  aGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCa                                                              <  1:3066768/100‑1 (MQ=255)

                                   gTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAc                                                             >  2:3001200/1‑100 (MQ=255)

                                     aaTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCATCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAccc                                                           <  1:2079930/100‑1 (MQ=255)

                                     aaTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAccc                                                           <  2:1758048/100‑1 (MQ=255)

                                         ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAg                                                         >  1:3226225/1‑98 (MQ=255)

                                         ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTc                                                       >  1:2326602/1‑100 (MQ=255)

                                         ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTc                                                       >  2:1007904/1‑100 (MQ=255)

                                          gCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCa                                                      >  2:1104433/1‑100 (MQ=255)

                                             cAATACAGCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTg                                                   <  1:3986557/100‑1 (MQ=255)

                                               aTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAg                                                 <  1:2612582/100‑1 (MQ=255)

                                                  cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcgc                                              >  2:3090468/1‑100 (MQ=255)

                                                  cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcgc                                              >  2:203345/1‑100 (MQ=255)

                                                  cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcgc                                              >  1:2245197/1‑100 (MQ=255)

                                                   aTCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCa                                             >  2:2950975/1‑100 (MQ=255)

                                                    tCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAt                                            <  2:966385/100‑1 (MQ=255)

                                                     ccAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAtt                                           <  2:2560105/100‑1 (MQ=255)

                                                      cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                          <  1:2500634/100‑1 (MQ=255)

                                                      cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                          >  2:3411992/1‑100 (MQ=255)

                                                      cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                          >  2:3392960/1‑100 (MQ=255)

                                                       aGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTaa                                         >  1:3574916/1‑100 (MQ=255)

                                                          gCTTCCCGCCCTTCGCGCAGCCATTGCGGGTCGGCCAGAACGGCAGAAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGTGCGCATTAAAc                                       <  1:787002/99‑1 (MQ=255)

                                                          gCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACa                                      <  1:4078427/100‑1 (MQ=255)

                                                          gCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACa                                      >  2:32377/1‑100 (MQ=255)

                                                          gCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACa                                      >  2:2125680/1‑100 (MQ=255)

                                                          gCTTCCCGCCCGTCGCGCAGCAAGTGCGGTTCGGCCAGACCGGCAGCATTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAAc                                       <  2:3874067/99‑1 (MQ=255)

                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCGAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCACATTAAACAGCg                                   <  1:3001188/100‑1 (MQ=255)

                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCATAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCATATTTAACAGCg                                   <  1:2242267/100‑1 (MQ=255)

                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                   >  1:1013300/1‑100 (MQ=255)

                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                   <  2:2663792/100‑1 (MQ=255)

                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                   <  1:347973/100‑1 (MQ=255)

                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                   >  1:186050/1‑100 (MQ=255)

                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                   >  2:1650659/1‑100 (MQ=255)

                                                              cccGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGt                                  <  2:3066825/100‑1 (MQ=255)

                                                               ccGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGt                                  <  1:272161/99‑1 (MQ=255)

                                                               ccGCCCTTCGCGCAGCAATTGCGGGCCGGCCAGAACGGCAGCAGTAAGGCGATCGCTGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGc                                    <  1:1052677/97‑1 (MQ=255)

                                                                   ccTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGt                             >  1:3339180/1‑100 (MQ=255)

                                                                    cTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGt                             <  2:1035689/99‑1 (MQ=255)

                                                                       cgcgCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcat                         >  2:2921715/1‑100 (MQ=255)

                                                                       cgcgCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcat                         <  2:3130794/100‑1 (MQ=255)

                                                                       cgcgCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtc                           >  1:3322827/1‑98 (MQ=255)

                                                                        gcgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatc                        <  2:3072688/100‑1 (MQ=255)

                                                                         cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca                       >  2:901296/1‑100 (MQ=255)

                                                                              caATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAcc                  >  2:1763876/1‑100 (MQ=255)

                                                                               aaTTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccg                 <  2:3981096/100‑1 (MQ=255)

                                                                                aTTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgc                >  1:3221436/1‑100 (MQ=255)

                                                                                aTTGCGGGTCGGCCAGAACGGCACCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgc                <  1:16158/100‑1 (MQ=255)

                                                                                  tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg              >  2:2468360/1‑100 (MQ=255)

                                                                                  tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg              <  2:293858/100‑1 (MQ=255)

                                                                                   gCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCgg             <  1:2745008/100‑1 (MQ=255)

                                                                                    cGGGCCGGCCAGATCGGCAGCAGTAATGCGATCGCGTTAGTACCGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGa            <  2:4128697/100‑1 (MQ=255)

                                                                                     gggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGAt           <  1:3228644/100‑1 (MQ=255)

                                                                                       gTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTc         >  1:708664/1‑100 (MQ=255)

                                                                                          ggCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAcc      >  1:3197366/1‑100 (MQ=255)

                                                                                          ggCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAcc      >  1:1123035/1‑100 (MQ=255)

                                                                                           gCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCg     <  1:52596/100‑1 (MQ=255)

                                                                                            ccAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGc    >  1:832627/1‑100 (MQ=255)

CTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCC  >  NC_000913/1826934‑1827127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A