Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,835,227 T→C 80.6% F140F (TTT→TTC)  ydjZ → TVP38/TMEM64 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,835,2270TC80.6% 282.5 / 56.4 135F140F (TTT→TTC) ydjZTVP38/TMEM64 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (11/15);  new base C (55/53);  total (67/68)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01

Only 100 of 140 total aligned reads displayed.

CCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATA  >  NC_000913/1835130‑1835323
                                                                                                 |                                                                                                
ccGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt                                                                                                <  1:3647117/100‑1 (MQ=255)
 cggcgcggcgCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttcttc                                                                                               <  1:2443712/100‑1 (MQ=255)
   gcgcggcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCAc                                                                                             <  1:1179567/100‑1 (MQ=255)
   gcgcggcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCAc                                                                                             <  1:815941/100‑1 (MQ=255)
    cgcggcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCAc                                                                                             <  2:2591334/99‑1 (MQ=255)
    cgcggcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACt                                                                                            >  1:3710300/1‑100 (MQ=255)
    cgcggcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACt                                                                                            <  1:3538905/100‑1 (MQ=255)
      cggcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCg                                                                                          >  1:1397644/1‑100 (MQ=255)
       ggcgaagaacTTTTTTATAGCCAGAGTAAGGGGCGGCGAAGTCGTGGAAAAATTAACCGGCAAACCCGTGCTAGCAGGTATGTACGGCGTGTTCACACg                                                                                          >  2:3110007/10‑99 (MQ=255)
        gcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCGGTGACGTGGTGGAAAAATTAACCGGCCATACCGGGCTTGACAGTATGGACGGCTTCTTCACTc                                                                                           <  2:500556/97‑1 (MQ=255)
        gcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCt                                                                                        >  2:2419918/1‑100 (MQ=255)
         cgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTa                                                                                       <  2:3463848/100‑1 (MQ=255)
           cTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACg                                                                                     >  2:1929496/1‑100 (MQ=255)
              tgCTTTTTTATCGCAAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCa                                                                                  <  2:2836235/100‑1 (MQ=255)
               gCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCaa                                                                                 >  1:1587631/1‑100 (MQ=255)
                cTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCaaa                                                                                >  2:3485000/1‑100 (MQ=255)
                cTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCaaa                                                                                <  1:3206160/100‑1 (MQ=255)
                cTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCaa                                                                                 >  2:4074408/1‑99 (MQ=255)
                 ttttttATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAac                                                                               <  1:1736123/100‑1 (MQ=255)
                  tttttATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAaca                                                                              <  2:3355907/100‑1 (MQ=255)
                    tttATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAacaca                                                                            >  1:3939438/1‑100 (MQ=255)
                         cGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACATTATGTACGGCTGCTTGCCTCGCTACGGCAAACACACCa                                                                         >  2:402716/1‑98 (MQ=255)
                          gCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTc                                                                      >  2:761920/1‑100 (MQ=255)
                           ccAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCt                                                                     >  2:569650/1‑100 (MQ=255)
                            cAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTg                                                                    >  2:3181572/1‑100 (MQ=255)
                             agagTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTgg                                                                   <  1:2064048/100‑1 (MQ=255)
                              gagTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGt                                                                  >  1:835029/1‑100 (MQ=255)
                              gagTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGt                                                                  <  1:3076801/100‑1 (MQ=255)
                               agTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTc                                                                 <  1:33094/100‑1 (MQ=255)
                               agTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTc                                                                 <  1:2225085/100‑1 (MQ=255)
                               agTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTc                                                                 <  1:3326356/100‑1 (MQ=255)
                               agTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTc                                                                 <  2:408363/100‑1 (MQ=255)
                                gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCt                                                                <  1:2246623/100‑1 (MQ=255)
                                gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCt                                                                <  1:278792/100‑1 (MQ=255)
                                 tGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTg                                                               >  1:221579/1‑100 (MQ=255)
                                   aTGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTc                                                             <  1:2604734/100‑1 (MQ=255)
                                   aTGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTc                                                             >  2:409051/1‑100 (MQ=255)
                                       gCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCCTTCTGGTCTGTCGGt                                                          >  1:709524/1‑99 (MQ=255)
                                       gCCGCGAAGTGGAGGAAAAATTAACCGGCAAAACCGTGCTTGAGAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGtt                                                         >  2:681385/1‑100 (MQ=255)
                                         cgcgAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTAt                                                       <  2:2245649/100‑1 (MQ=255)
                                         cgcgAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTAt                                                       <  1:2390338/100‑1 (MQ=255)
                                          gcgAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTAtt                                                      <  2:1781360/100‑1 (MQ=255)
                                               gtggtgGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCtt                                                 <  2:295033/100‑1 (MQ=255)
                                                tggtggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCttt                                                <  2:1636479/100‑1 (MQ=255)
                                                 ggtggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCtttt                                               >  2:978882/1‑100 (MQ=255)
                                                  gtggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTAACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGCTATTGCCTTTTg                                              >  2:1035521/1‑100 (MQ=255)
                                                   tggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGt                                             <  1:2613651/100‑1 (MQ=255)
                                                   tggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGt                                             >  2:1262917/1‑100 (MQ=255)
                                                   tggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGt                                             >  2:2038141/1‑100 (MQ=255)
                                                    ggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTc                                            <  1:3495256/100‑1 (MQ=255)
                                                    ggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTc                                            <  1:3769056/100‑1 (MQ=255)
                                                     gAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTcc                                           >  1:4095604/1‑100 (MQ=255)
                                                     gAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTcc                                           <  2:654804/100‑1 (MQ=255)
                                                     gAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTcc                                           <  1:2389462/100‑1 (MQ=255)
                                                         aaTTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCttt                                       <  1:3273568/100‑1 (MQ=255)
                                                          aTTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTc                                      <  2:1875782/100‑1 (MQ=255)
                                                           ttAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCg                                     <  2:3337153/100‑1 (MQ=255)
                                                            tAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGa                                    <  1:342657/100‑1 (MQ=255)
                                                               ccGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATcc                                 <  1:3397976/100‑1 (MQ=255)
                                                               ccGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATcc                                 >  2:3593745/1‑100 (MQ=255)
                                                                cGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCa                                >  1:293646/1‑100 (MQ=255)
                                                                cGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCa                                >  1:3657844/1‑100 (MQ=255)
                                                                  gCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAAt                              >  2:1104190/1‑100 (MQ=255)
                                                                  gCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACAAACCATTCCGGCCTTTCGGTGATTGCCTTCTGTCCCTTTCGATCCa                                >  2:1436956/1‑98 (MQ=255)
                                                                   cAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATc                             >  2:3027321/1‑100 (MQ=255)
                                                                    aaaaCCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAAt                              <  2:1635062/98‑1 (MQ=255)
                                                                    aaaaCCGTGCTTGACAGTATGGACGGCTTCTTCACTCCCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCa                            >  2:2224997/1‑100 (MQ=255)
                                                                     aaaCCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAg                           >  2:298044/1‑100 (MQ=255)
                                                                     aaaCCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAg                           <  1:2517768/100‑1 (MQ=255)
                                                                     aaaCCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAg                           >  2:57371/1‑100 (MQ=255)
                                                                      aaCCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTTGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGc                          >  2:1060788/1‑100 (MQ=255)
                                                                      aaCCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGc                          <  1:50769/100‑1 (MQ=255)
                                                                       aCCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCt                         >  2:2161503/1‑100 (MQ=255)
                                                                        ccGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTa                        <  1:2389300/100‑1 (MQ=255)
                                                                        ccGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTa                        <  1:25972/100‑1 (MQ=255)
                                                                          gatcTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTAtg                      <  2:3817482/97‑1 (MQ=255)
                                                                           tGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTAtgc                     <  2:872366/100‑1 (MQ=255)
                                                                             cTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTAtgctg                   >  1:3290187/1‑100 (MQ=255)
                                                                             cTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCATTCGATCCAATCAGCTAtgctg                   >  2:250366/1‑100 (MQ=255)
                                                                               tGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTAtgagatc                 >  1:3817511/1‑95 (MQ=255)
                                                                                gACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCg                >  1:11288/1‑100 (MQ=255)
                                                                                     tATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTg           <  1:3972138/100‑1 (MQ=255)
                                                                                     tATGGACGGCTTCTTCACTCGCTACGGCAAACACACCCTTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTg           <  1:3751267/100‑1 (MQ=255)
                                                                                      aTGGACGGCTTCTTCACTCCCTACGGCAAACACACCATTCTGGTCTTTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGttt            <  2:3665765/98‑1 (MQ=255)
                                                                                       tGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGAc         <  1:1185807/100‑1 (MQ=255)
                                                                                        ggACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACt        >  2:1365145/1‑100 (MQ=255)
                                                                                         gACGGCTTCTTCACTCGCTACGGCAAACACACCCTTCTGGTCTGTCGCTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTCTGCTGCCGGCTTGACt        >  2:2880566/1‑99 (MQ=255)
                                                                                          aCGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTc      >  1:387085/1‑100 (MQ=255)
                                                                                          aCGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTc      <  2:3954848/100‑1 (MQ=255)
                                                                                           cGGCTTTTTTATTCTTTACGACAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGTTTTGACTTc      >  2:4138628/1‑99 (MQ=255)
                                                                                           cGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATAAGCTATGCTGCCGGTTTGACTTCa     <  1:3119243/100‑1 (MQ=255)
                                                                                           cGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGGTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATTCTGCCGGTTTGACTTCa     >  2:4141373/1‑100 (MQ=255)
                                                                                            ggCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCaa    <  2:1505318/100‑1 (MQ=255)
                                                                                             gCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAAt   <  1:2830317/100‑1 (MQ=255)
                                                                                             gCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAAt   >  2:272542/1‑100 (MQ=255)
                                                                                             gCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAAt   >  2:2625413/1‑100 (MQ=255)
                                                                                              cttcttCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATa  >  2:1466703/1‑100 (MQ=255)
                                                                                              cttcttCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATa  >  1:3804142/1‑100 (MQ=255)
                                                                                              cTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATa  >  2:3469789/1‑100 (MQ=255)
                                                                                              cTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATa  >  1:3198170/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATA  >  NC_000913/1835130‑1835323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A