Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F204 I0 R1 247 174.3 8556966 94.2% 8060661 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,835,227 T→C 39.4% F140F (TTT→TTC)  ydjZ → TVP38/TMEM64 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,835,2270TC39.4% 105.7 / 167.2 160F140F (TTT→TTC) ydjZTVP38/TMEM64 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (45/52);  new base C (31/32);  total (76/84)
Fisher's exact test for biased strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 168 total aligned reads displayed.

GGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACG  >  NC_000913/1835128‑1835325
                                                                                                   |                                                                                                  
ggCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttt                                                                                                    >  2:700599/1‑100 (MQ=255)
 gCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCtttt                                                                                                   >  2:1057014/1‑100 (MQ=255)
   cggcgcggcgCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttcttc                                                                                                 <  2:3462395/100‑1 (MQ=255)
   cggcgcggcgCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTc                                                                                                 <  2:3652810/100‑1 (MQ=255)
     gcgcggcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCAc                                                                                               <  2:4165120/100‑1 (MQ=255)
      cgcggcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACt                                                                                              <  1:3874459/100‑1 (MQ=255)
       gcggcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACtn                                                                                             <  1:275351/100‑2 (MQ=255)
        cggcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCg                                                                                            <  2:2740195/100‑1 (MQ=255)
        cggcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCg                                                                                            >  1:1025621/1‑100 (MQ=255)
        cggcgcTGTACTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCg                                                                                            <  1:160978/100‑1 (MQ=255)
          gcgcTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCt                                                                                          <  2:1883744/100‑1 (MQ=255)
             cTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACg                                                                                       >  2:3042368/1‑100 (MQ=255)
              tgtgCTTTGTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCGTGACAGTATGGACGGCTTCTTCACTCGCTACgg                                                                                      <  1:1243576/100‑1 (MQ=255)
               gtgCTTTTTTATCGCCAGCGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCTTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGc                                                                                     <  1:2644031/100‑1 (MQ=255)
                tgCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTTGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCa                                                                                    >  1:1055498/1‑100 (MQ=255)
                 gCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCaa                                                                                   >  1:1830060/1‑100 (MQ=255)
                  cTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCaaa                                                                                  >  2:1828455/1‑100 (MQ=255)
                  cTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCaa                                                                                   >  2:643919/1‑99 (MQ=255)
                    tttttATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAaca                                                                                <  1:2079133/100‑1 (MQ=255)
                      tttATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAacaca                                                                              >  1:2432338/1‑100 (MQ=255)
                        tATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACAcc                                                                            >  2:2475876/1‑100 (MQ=255)
                         aTCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCa                                                                           >  2:2055676/1‑100 (MQ=255)
                           cGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCAtt                                                                         >  1:1590273/1‑100 (MQ=255)
                           cGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCAtt                                                                         >  2:1008735/1‑100 (MQ=255)
                            gCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTc                                                                        >  2:343546/1‑100 (MQ=255)
                             ccAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGCCGGCTTCTTCACTCGCTACGGCAAACACACCATTCt                                                                       >  1:1998094/1‑100 (MQ=255)
                             ccAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCt                                                                       <  2:3474666/100‑1 (MQ=255)
                             ccAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCt                                                                       <  2:3272946/100‑1 (MQ=255)
                             ccAGAGTGATGGGCCGCGAAGTGGTGGAAAAAGTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCt                                                                       <  2:1489400/100‑1 (MQ=255)
                               agagTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTgg                                                                     <  1:808725/100‑1 (MQ=255)
                               agagTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTgg                                                                     <  2:1921478/100‑1 (MQ=255)
                                gagTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGt                                                                    >  1:2852008/1‑100 (MQ=255)
                                gagTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGt                                                                    >  1:2253917/1‑100 (MQ=255)
                                 attGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTc                                                                   <  1:2978313/98‑1 (MQ=255)
                                    gATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGt                                                                >  1:2133215/1‑100 (MQ=255)
                                    gATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGt                                                                >  2:1378721/1‑100 (MQ=255)
                                     aTGGGCCGAGAAGTGGTGGAAAAATTAACCAGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCt                                                                       <  1:2303824/92‑1 (MQ=255)
                                      tGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCg                                                              >  1:398920/1‑100 (MQ=255)
                                       gggCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCgg                                                             >  1:3512907/1‑100 (MQ=255)
                                       gggCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCgg                                                             >  1:3512846/1‑100 (MQ=255)
                                       gggCCGCGAAGTGGAGGAAAAATTACCCGGCAAACCCGTGCTTGACAGTATAGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCgg                                                             <  1:1519771/100‑1 (MQ=255)
                                        ggCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGt                                                            <  1:1450800/100‑1 (MQ=255)
                                             cgAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTg                                                       <  2:1109962/100‑1 (MQ=255)
                                                  tggtggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCttt                                                  <  1:190921/100‑1 (MQ=255)
                                                   ggtggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCtttt                                                 >  1:1967189/1‑100 (MQ=255)
                                                   ggtggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCGTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGGTATTGCCt                                                    >  1:3884507/1‑97 (MQ=255)
                                                   ggtggAAAAATTAACCGGCAAAACCGAGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCtttt                                                 >  2:2593296/1‑100 (MQ=255)
                                                     tggAAAAATTAACCGGCAAATCCGTGCTTGACAGTATGGACGGCTTCTTCCCTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGt                                               <  1:626027/100‑1 (MQ=255)
                                                     tggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGt                                               <  2:3702562/100‑1 (MQ=255)
                                                     tggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGt                                               >  2:2461435/1‑100 (MQ=255)
                                                      ggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTTTTTTTTTTTTTCAAACACACCATTCTGGTCTGTCGTTTATTGCCTTTTGTc                                              >  1:4147088/1‑100 (MQ=255)
                                                      ggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTc                                              >  2:3106804/1‑100 (MQ=255)
                                                       gtaaaaTTAACTGCCAAGTCCTTGCTTGACAGTATGCACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTcc                                             <  1:3730155/98‑1 (MQ=255)
                                                       gAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTcc                                             >  2:1338172/1‑100 (MQ=255)
                                                       gAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGAAGGCTTTTTCCCTCGCTACATCAAACACACCATTCTGGTCTGGCTGTTATTGCATGTTGTcc                                             <  1:859635/100‑1 (MQ=255)
                                                        aaaaaTTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTccc                                            >  2:2864403/1‑100 (MQ=255)
                                                        aaaaaTTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTccc                                            <  1:1798582/100‑1 (MQ=255)
                                                         aaaaTTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCt                                           <  1:2837009/100‑1 (MQ=255)
                                                            aTTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTc                                        <  1:1721915/100‑1 (MQ=255)
                                                            aTTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAACCACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTc                                        >  2:593686/1‑100 (MQ=255)
                                                             ttAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCg                                       <  2:3367897/100‑1 (MQ=255)
                                                             ttAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCg                                       >  2:4276726/1‑100 (MQ=255)
                                                               aaCCGGGAAAACCGTGCTTGACAGTGTGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCGTTTGTCCCTTTCGAt                                     <  1:3429709/100‑1 (MQ=255)
                                                               aaCCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGAt                                     <  2:3134603/100‑1 (MQ=255)
                                                               aaCCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGAt                                     <  2:1705538/100‑1 (MQ=255)
                                                               aaCCGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGAt                                     <  2:1542083/100‑1 (MQ=255)
                                                                 ccGGCATATCCGTGCTTGACAGTATGGACGGCTTTTTCTCTCGCTACGGCCAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATc                                    <  1:1324116/99‑1 (MQ=255)
                                                                 ccGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATcc                                   <  1:2154150/100‑1 (MQ=255)
                                                                 ccGGCAAAACCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATcc                                   <  2:4099377/100‑1 (MQ=255)
                                                                      aaaaCCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACTGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCa                              <  2:3191965/100‑1 (MQ=255)
                                                                      aaaaCCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCa                              <  1:700596/100‑1 (MQ=255)
                                                                         aCCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCt                           >  1:2505147/1‑100 (MQ=255)
                                                                         aCCGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCt                           <  1:4169385/100‑1 (MQ=255)
                                                                          ccGTGCTTGACAGTATGGACGGCTTTTTTATTCGCTACGGCAAACACACCATGCTGTTCTGTCTGTTATGGCTTTTTGTCCCTTTCGATCCAATCAGCTa                          >  1:1419978/1‑100 (MQ=255)
                                                                          ccGTGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTa                          >  2:2293522/1‑100 (MQ=255)
                                                                            gTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTAtg                        >  1:3251895/1‑100 (MQ=255)
                                                                             tGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTAtgc                       <  2:510267/100‑1 (MQ=255)
                                                                             tGCTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTAtgc                       >  2:1513107/1‑100 (MQ=255)
                                                                              gCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTAtgct                      >  2:4070108/1‑100 (MQ=255)
                                                                               cTTGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTAtgctg                     >  2:3902569/1‑100 (MQ=255)
                                                                                ttGACAGTATGGACGGCTTCTTCACTCGCCACGTCAAACACACCATTCTGGTCTGTCGGTGATTGCCTTTTGACCCTTTAGATCCAATCCGCTAtgctg                     >  1:481868/1‑99 (MQ=255)
                                                                                 tGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGcc                   >  2:1980954/1‑100 (MQ=255)
                                                                                 tGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGcc                   <  1:1199948/100‑1 (MQ=255)
                                                                                 tGACAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGcc                   <  2:1480041/100‑1 (MQ=255)
                                                                                   aCAGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCgg                 <  1:2862060/100‑1 (MQ=255)
                                                                                     aGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGtt               >  2:3126125/1‑100 (MQ=255)
                                                                                     aGTATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGtt               <  1:765527/100‑1 (MQ=255)
                                                                                       tATGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTg             <  1:3239685/100‑1 (MQ=255)
                                                                                        aTGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGa            <  2:278079/100‑1 (MQ=255)
                                                                                        aTGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGa            <  2:355402/100‑1 (MQ=255)
                                                                                         tGGACGGCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGAc           >  1:1439261/1‑100 (MQ=255)
                                                                                          ggACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACt          >  2:3222030/1‑100 (MQ=255)
                                                                                           gACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACtt         >  2:2080555/1‑100 (MQ=255)
                                                                                           gACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACtt         >  1:898425/1‑100 (MQ=255)
                                                                                            aCGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTc        >  1:3170400/1‑100 (MQ=255)
                                                                                             cGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCa       >  2:782854/1‑100 (MQ=255)
                                                                                               gCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAAt     >  2:4120536/1‑100 (MQ=255)
                                                                                               gCTTCTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAAt     >  2:1808551/1‑100 (MQ=255)
                                                                                                cttcttCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATa    <  1:4200628/100‑1 (MQ=255)
                                                                                                cTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATa    >  2:3314307/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
GGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGTTATTGCCTTTTGTCCCTTTCGATCCAATCAGCTATGCTGCCGGTTTGACTTCAATACG  >  NC_000913/1835128‑1835325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A