Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F142 I0 R1
|
175 |
91.2 |
4812830 |
94.5% |
4548124 |
99.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
2,541,456 |
A→G |
22.1% |
I75T (ATT→ACT) |
cysW ← |
sulfate/thiosulfate ABC transporter permease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,541,456 | 0 | A | G | 22.1%
| 147.5
/ 41.5
| 81 | I75T (ATT→ACT) | cysW | sulfate/thiosulfate ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (29/34); new base G (6/12); total (35/46) |
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 |
CAGTAGCGTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGCTGAATGCCTGCACG > NC_000913/2541362‑2541554
|
cAGTAGCGTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAgc > 2:774765/1‑100 (MQ=255)
tatcGTTAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGcgcg < 1:606997/97‑1 (MQ=255)
tAGCGTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGcgcg < 1:1716641/100‑1 (MQ=255)
gCGTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGAAATCAGCGCGat > 2:206867/1‑100 (MQ=255)
gTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAGTCAGCGCGatca < 1:1819238/100‑1 (MQ=255)
tCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGatcat < 1:57775/100‑1 (MQ=255)
gcagTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCAc > 1:1068854/1‑100 (MQ=255)
gcagTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCAc < 1:2180667/100‑1 (MQ=255)
cagTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCAcc > 1:887830/1‑100 (MQ=255)
agTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGTCAATCAGCGCGATCATCAc < 2:168255/98‑1 (MQ=255)
gTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGTAGAATGCCGAACACCAGGTTTACCGGTACGGCAATTAGAGCGATCATCAcc < 2:831096/98‑1 (MQ=255)
gTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGt > 2:1064341/1‑100 (MQ=255)
tAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTc > 2:1046780/1‑100 (MQ=255)
naCTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCa > 1:988624/2‑100 (MQ=255)
cTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGc < 1:1173104/100‑1 (MQ=255)
tGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGcc < 1:2227544/100‑1 (MQ=255)
cGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGa > 1:1524709/1‑100 (MQ=255)
cGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGa > 1:1992884/1‑100 (MQ=255)
cGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGa > 1:1446788/1‑100 (MQ=255)
aGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCg < 2:1501759/100‑1 (MQ=255)
ggAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACTCCAGGTTTACCGGTACGGCAATCAGCGCGATCATCTCCGTCAGCCAGATGGCg < 2:37853/98‑1 (MQ=255)
gAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTgc < 1:2352418/100‑1 (MQ=255)
aaGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgca < 1:1240286/100‑1 (MQ=255)
aaGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgca < 1:244211/100‑1 (MQ=255)
aGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTgcag > 1:357445/1‑100 (MQ=255)
aGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgcag < 1:1751766/100‑1 (MQ=255)
gTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTgcagc > 1:1908461/1‑100 (MQ=255)
ttAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTgcagca > 2:2150023/1‑100 (MQ=255)
tAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCAt > 2:1492392/1‑100 (MQ=255)
aaGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGt < 1:2048998/100‑1 (MQ=255)
aGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTc > 1:1972979/1‑100 (MQ=255)
gcgcGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTcc > 2:299177/1‑100 (MQ=255)
cgcgTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCg < 2:2237080/100‑1 (MQ=255)
cgTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGa > 1:2129111/1‑100 (MQ=255)
gTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACTGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGAt < 1:1646576/100‑1 (MQ=255)
gTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGAt > 2:2323870/1‑100 (MQ=255)
ccagccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGc > 1:1450763/1‑100 (MQ=255)
agccagGCCAGCAGAATGCCGAACAGCAGGTGTACCGGTACGGCAGTCAGCGCGATCAGCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGcc < 1:812961/99‑1 (MQ=255)
agccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGcc < 1:797349/99‑1 (MQ=255)
agccagGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCa < 2:1068844/100‑1 (MQ=255)
gccaggccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCa > 1:295022/1‑99 (MQ=255)
ngccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTc > 1:2150879/2‑100 (MQ=255)
ggccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTc > 1:1732424/1‑100 (MQ=255)
gccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCt < 2:2058896/100‑1 (MQ=255)
ccagCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTg > 1:1530998/1‑100 (MQ=255)
cagcagAATGCCGAACACCAGGTTTACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTATGt > 2:714500/1‑100 (MQ=255)
cagcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGt < 1:1277439/100‑1 (MQ=255)
cagcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGt < 2:24825/100‑1 (MQ=255)
agcagAATGCCGAACACCAGGTTTACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTa < 2:1077475/100‑1 (MQ=255)
agcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGt < 1:962644/99‑1 (MQ=255)
agcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTa > 2:726148/1‑100 (MQ=255)
gcagAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGt < 1:1591709/98‑1 (MQ=255)
aaTGCCGAACACCAGGTTTACCGGTACGGCAGTCAGCACGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAAcc < 2:1614441/100‑1 (MQ=255)
aaTGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAAcc < 1:124741/100‑1 (MQ=255)
aTGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCg > 2:2100958/1‑100 (MQ=255)
tGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCgg < 2:1058174/100‑1 (MQ=255)
ccGAACACCAGGTTTACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCa < 2:1450747/100‑1 (MQ=255)
ccGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCa < 2:585449/100‑1 (MQ=255)
ccGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCa < 1:1064351/100‑1 (MQ=255)
cGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCa < 2:728748/99‑1 (MQ=255)
cGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCAt < 2:293130/100‑1 (MQ=255)
acacCAGGTTTACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAg < 1:614158/100‑1 (MQ=255)
acCAGGTTTACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGcc < 2:2399900/100‑1 (MQ=255)
acCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCCGCATCAGcc > 1:533497/1‑100 (MQ=255)
ccAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGccc > 1:2075438/1‑100 (MQ=255)
ccAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGccc > 1:709744/1‑100 (MQ=255)
ccAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGccc > 2:651401/1‑100 (MQ=255)
cAGGTGTGCCGGTGCGGCAATCAGCGCGTTCATCACCGACAGCCAGATGGGGTGCAGCATGTCTGGATCGGCCAAATTCTGGAAAACCGGCATCAGcc < 2:1666690/98‑1 (MQ=255)
aGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCt < 2:1726949/100‑1 (MQ=255)
tttACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGc > 1:1330669/1‑100 (MQ=255)
tttACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGc < 1:2296157/100‑1 (MQ=255)
ttACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGCt < 1:2332048/100‑1 (MQ=255)
tACCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGCTg < 2:750679/100‑1 (MQ=255)
aCCGGTACGGCAGTCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGCTGa < 1:1434373/100‑1 (MQ=255)
ggTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGCTGAATg > 1:1381207/1‑100 (MQ=255)
tACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAATCCGGCATCAGCCCCTTGCTGAATGcc < 2:195032/100‑1 (MQ=255)
tACGGCAATCAGCGCGAGCAGCACCGTCAGCCAGATGGCGTGCAGCATGTACGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGCTGAATGcc < 2:2361579/100‑1 (MQ=255)
aCGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGCTGAATGCCt > 1:2069471/1‑100 (MQ=255)
aCGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGCTGAATGCCt > 2:1642295/1‑100 (MQ=255)
gCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGCTGAATGCCTGCa > 2:1978228/1‑100 (MQ=255)
cAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGCTGAATGCCTGCAc < 1:1154347/100‑1 (MQ=255)
aaTCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGCTGAATGCCTGCACg < 2:1503457/100‑1 (MQ=255)
|
CAGTAGCGTCAGCAGTAACTGGCGTCCAGGGAAGTTAAAGCGCGTCACCAGCCAGGCCAGCAGAATGCCGAACACCAGGTTTACCGGTACGGCAATCAGCGCGATCATCACCGTCAGCCAGATGGCGTGCAGCATGTCCGGATCGGCCAGATTCTGTAAAACCGGCATCAGCCCCTTGCTGAATGCCTGCACG > NC_000913/2541362‑2541554
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
