Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F133 I0 R1	200	171.4	8351354	94.3%	7875326	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,501,197	A→C	79.3%	N23T (AAC→ACC)	ypdC →	putative DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,501,197	0	A	C	79.3%	295.9 / 63.3	124	N23T (AAC→ACC)	ypdC	putative DNA‑binding protein
Reads supporting (aligned to +/- strand): ref base A (8/18); new base C (50/48); total (58/66)
Fisher's exact test for biased strand distribution p-value = 7.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

AGTTAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGTGCT  >  NC_000913/2501101‑2501293

aGTTAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAcccc                                                                                               >  1:1303584/1‑100 (MQ=255)

  ttAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCAGATCAACAATTTCTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGc                                                                                             <  2:1043466/100‑1 (MQ=255)

   tAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCa                                                                                            <  1:2579248/100‑1 (MQ=255)

   tAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCa                                                                                            <  2:1485227/100‑1 (MQ=255)

   tAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCa                                                                                            >  2:553220/1‑100 (MQ=255)

    aaTGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCaa                                                                                           >  2:1016194/1‑100 (MQ=255)

    aaTGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCaa                                                                                           >  2:490773/1‑100 (MQ=255)

      ttccgatctAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAact                                                                                         <  1:510285/92‑1 (MQ=255)

       gCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCACGCCGATCAACAATTTTTCGCCGATCTGTTCAGAGGCCTGGTGCTTACTCCGCAact                                                                                         >  2:3924363/1‑99 (MQ=255)

        cATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAactac                                                                                       >  1:1749259/1‑100 (MQ=255)

           cTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTgg                                                                                    >  1:11316/1‑100 (MQ=255)

           cTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTgg                                                                                    <  2:3171013/100‑1 (MQ=255)

            tttAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTggg                                                                                   <  1:434502/100‑1 (MQ=255)

            tttAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTggg                                                                                   <  1:3517361/100‑1 (MQ=255)

             ttAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTggg                                                                                   >  1:2429761/1‑99 (MQ=255)

             ttAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTAGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTgg                                                                                    >  2:2756441/1‑98 (MQ=255)

               aaTCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAactagatcggaa                                                                                >  2:510282/1‑92 (MQ=255)

                aTCGGGAACTTTCATGAAAGCACCAGGCTTGCCTGCCGATCAACAATTTTTCGCCGATTTGGTCAGCGGCCTGGTGCTTTAACCGCAACGACTGGGgcgc                                                                               >  1:610289/1‑100 (MQ=255)

                   gggAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTc                                                                            <  1:2613268/100‑1 (MQ=255)

                      aaCTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTgg                                                                         <  1:3622164/100‑1 (MQ=255)

                        cTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGtt                                                                       >  1:2843709/1‑100 (MQ=255)

                        cTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGt                                                                        <  2:2075979/99‑1 (MQ=255)

                         tttCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACACCGCAACTACTGGGGCGCGTCTGGttt                                                                      >  2:1476013/1‑100 (MQ=255)

                          ttCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTg                                                                     <  1:3618178/100‑1 (MQ=255)

                            cATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCAGCAACTACTGGGGCGCGTCTGGTTTgcc                                                                   >  2:4154028/1‑100 (MQ=255)

                             aTGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTgcca                                                                  >  1:1009158/1‑100 (MQ=255)

                              tGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTgccag                                                                 >  1:1058491/1‑100 (MQ=255)

                              tGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTgccag                                                                 >  1:1491515/1‑100 (MQ=255)

                               gAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTgccagc                                                                >  1:1668998/1‑100 (MQ=255)

                                aaaGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTgccagcc                                                               <  1:3228797/100‑1 (MQ=255)

                                   gCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCcagccagc                                                            >  1:3812723/1‑100 (MQ=255)

                                     aCCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAgcct                                                          <  1:409252/100‑1 (MQ=255)

                                         ggCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGAGGCGCGTCTGGTTTGCCAGCCAgcctgc                                                        >  2:1850758/1‑98 (MQ=255)

                                          gCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTc                                                     <  1:3252436/100‑1 (MQ=255)

                                              gCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTg                                                 <  1:438865/100‑1 (MQ=255)

                                              gCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTg                                                 <  1:2602468/100‑1 (MQ=255)

                                               cccGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGc                                                <  1:2493033/100‑1 (MQ=255)

                                               cccGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGc                                                <  1:570055/100‑1 (MQ=255)

                                                ccgccgATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGcc                                               >  1:3288877/1‑100 (MQ=255)

                                                 cgccgATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGcc                                               <  2:3083228/99‑1 (MQ=255)

                                                  gccgATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGcccg                                             >  2:1933733/1‑98 (MQ=255)

                                                  gccgATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCgg                                             <  1:3044390/100‑1 (MQ=255)

                                                  gccgATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCgg                                             <  2:1281267/100‑1 (MQ=255)

                                                  gccgATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCgg                                             <  1:1817244/100‑1 (MQ=255)

                                                   ccgATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGt                                            >  1:823386/1‑100 (MQ=255)

                                                   ccgATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGt                                            >  2:3245122/1‑100 (MQ=255)

                                                    cgctCAACAATGTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTg                                           <  2:4132632/97‑1 (MQ=255)

                                                    cgATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTg                                           <  1:3419086/100‑1 (MQ=255)

                                                     gATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTgg                                          >  2:474954/1‑100 (MQ=255)

                                                      aTCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTggg                                         <  2:3358159/100‑1 (MQ=255)

                                                      aTCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTggg                                         <  1:3742728/100‑1 (MQ=255)

                                                        caacatTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCa                                       <  2:1857156/100‑1 (MQ=255)

                                                        caacaaTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCa                                       >  2:2891318/1‑100 (MQ=255)

                                                         aacaaTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAg                                      >  2:3802832/1‑100 (MQ=255)

                                                         aacaaTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAg                                      >  2:4117476/1‑100 (MQ=255)

                                                           caaTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGtt                                    >  1:4093274/1‑100 (MQ=255)

                                                           caaTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGtt                                    <  1:1611809/100‑1 (MQ=255)

                                                           caaTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGtt                                    >  1:2355658/1‑100 (MQ=255)

                                                           caaTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGtt                                    >  1:1988168/1‑100 (MQ=255)

                                                           caaTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGtt                                    <  1:3713703/100‑1 (MQ=255)

                                                            aaTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCTTCGTTGCCGGTGGGCAGttt                                   <  2:2537550/100‑1 (MQ=255)

                                                             aTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTa                                  >  2:488652/1‑100 (MQ=255)

                                                                tttCGCCGATCGGTTCAGCGGCCTGTTGCTTACCCCGCAACGACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATg                                <  2:3278093/99‑1 (MQ=255)

                                                                  tCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTAt                             <  2:2130962/100‑1 (MQ=255)

                                                                    gacGATCTGTTCAACGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTg                           >  1:2830636/3‑100 (MQ=255)

                                                                     ccGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGa                          >  1:1060326/1‑100 (MQ=255)

                                                                        aTCTGTTCAGCGGCCTGGTGCTTACCCCTCAACTACTGGGGCGCGTCTGGTTTGCCAGCCATCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGAttt                       <  2:1853522/100‑1 (MQ=255)

                                                                          cTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTc                     >  2:1956540/1‑100 (MQ=255)

                                                                          cTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTc                     >  2:1956786/1‑100 (MQ=255)

                                                                           tGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTcc                    >  2:130640/1‑100 (MQ=255)

                                                                           tGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTcc                    <  2:2121264/100‑1 (MQ=255)

                                                                           tGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTcc                    <  1:1860015/100‑1 (MQ=255)

                                                                           tGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTc                     >  1:44528/1‑99 (MQ=255)

                                                                            gTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTccc                   <  2:1574423/100‑1 (MQ=255)

                                                                            gTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTccc                   >  1:3084130/1‑100 (MQ=255)

                                                                             ttCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTcccc                  <  1:3778354/100‑1 (MQ=255)

                                                                              tCAGCGGCCTGGTGCTTACCCCGCAATTACTGGGGTGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTTGGCAGTTTATGTATTGATTTTCCCCg                 <  2:924998/100‑1 (MQ=255)

                                                                              tCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCg                 <  2:3216552/100‑1 (MQ=255)

                                                                                aGCGGCCTGGTGGTTACCCCGCCACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCg                 <  2:619170/98‑1 (MQ=255)

                                                                                aGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTc               <  1:2142599/100‑1 (MQ=255)

                                                                                 gCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTTATTTTCCCCGt                <  2:1434614/98‑1 (MQ=255)

                                                                                  cGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTg             <  1:490580/100‑1 (MQ=255)

                                                                                  cGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTg             <  1:1339037/100‑1 (MQ=255)

                                                                                   ggCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTgg            <  1:4087607/100‑1 (MQ=255)

                                                                                    gCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGa           <  2:1690861/100‑1 (MQ=255)

                                                                                    gCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGa           <  1:490776/100‑1 (MQ=255)

                                                                                    gCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGa           <  1:3276097/100‑1 (MQ=255)

                                                                                     ccTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGat          >  1:1352848/1‑100 (MQ=255)

                                                                                     ccTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGat          >  1:1797184/1‑100 (MQ=255)

                                                                                     ccTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGAGTTTCCCCGTCTGGat          >  1:4107493/1‑100 (MQ=255)

                                                                                      cTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGata         >  1:646984/1‑100 (MQ=255)

                                                                                        ggTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATc       >  1:1717044/1‑100 (MQ=255)

                                                                                         gTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCg      >  2:585072/1‑100 (MQ=255)

                                                                                         gTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCg      <  1:481256/100‑1 (MQ=255)

                                                                                          tGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGA‑TTTCCCCGTCTGGATATCGtg    <  1:440188/100‑1 (MQ=255)

                                                                                          tGCTTACCCCGCAACGACTGGGGCGCTTCTGGTTTGCCAGCCAGCCTGCCTCGTTGTCGGTGGGCAGTTTATGTATTGATGTTCCTTATCTGGGTATCGt     <  2:219564/100‑1 (MQ=255)

                                                                                           ncTTACCCCGCAAATACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGtg    >  2:2581131/2‑100 (MQ=255)

                                                                                            cTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAACCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGtgc   >  2:2229775/1‑100 (MQ=255)

                                                                                            cTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGtgc   <  2:2830620/100‑1 (MQ=255)

                                                                                             ttACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGtgct  >  1:2314098/1‑100 (MQ=255)

AGTTAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGTGCT  >  NC_000913/2501101‑2501293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A