Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,195,102 G→A 76.6% intergenic (+277/‑39) ibsD → / → ibsE toxic membrane protein/toxic membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,195,1020GA76.6% 279.7 / 90.4 169intergenic (+277/‑39)ibsD/ibsEtoxic membrane protein/toxic membrane protein
Reads supporting (aligned to +/- strand):  ref base G (14/25);  new base A (48/79);  total (63/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

Only 100 of 169 total aligned reads displayed.

GACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTA  >  NC_000913/3195005‑3195199
                                                                                                 |                                                                                                  
gACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAAcc                                                                                                  <  1:3905873/100‑1 (MQ=255)
gACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCAGCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAAcc                                                                                                  <  2:2137888/100‑1 (MQ=255)
   cacTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑t                                                                                              >  2:2009884/3‑100 (MQ=255)
    gCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑Tc                                                                                             >  1:108934/1‑100 (MQ=255)
    gCTCGGGGAAGGCAAAATTGCCTCAGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑Tc                                                                                             <  2:3726890/100‑1 (MQ=255)
      tCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑Tccc                                                                                           <  1:3921952/100‑1 (MQ=255)
      tCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑Tccc                                                                                           <  1:1519630/100‑1 (MQ=255)
       cGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCg                                                                                          <  1:1689946/100‑1 (MQ=255)
        ggggCAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACACTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCg                                                                                          <  2:1384619/99‑1 (MQ=255)
        ggggAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCga                                                                                         <  2:3869574/100‑1 (MQ=255)
         gggAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCgaa                                                                                        <  1:3016283/100‑1 (MQ=255)
           gAAGGCAAAATTGCCTCTGGGAAAGCATTTCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCgaaga                                                                                      >  2:1842881/1‑100 (MQ=255)
              ggCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAgg                                                                                   <  2:3052891/100‑1 (MQ=255)
              ggCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAgg                                                                                   >  1:3702250/1‑100 (MQ=255)
              ggCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAgg                                                                                   >  2:1717548/1‑100 (MQ=255)
               gCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAggg                                                                                  <  2:2726929/100‑1 (MQ=255)
                 aaaaTTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGc                                                                                <  1:1466283/100‑1 (MQ=255)
                   aaTTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCt                                                                              <  1:1298312/100‑1 (MQ=255)
                     ttGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGGTAATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTg                                                                            <  2:1388934/100‑1 (MQ=255)
                     ttGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGTAGAAGGGGCCTTg                                                                            <  1:2832774/100‑1 (MQ=255)
                     ttGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTg                                                                            <  1:1169296/100‑1 (MQ=255)
                        ccTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGtat                                                                         <  2:793025/100‑1 (MQ=255)
                        ccTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGtat                                                                         >  1:2402758/1‑100 (MQ=255)
                        ccTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGtat                                                                         >  1:214709/1‑100 (MQ=255)
                        ccTCTGGGAAAGCATTGCGCGGGGTCCGGCGATCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAACCCCC‑TCCCGAAGAAGGGGCCTTGtat                                                                         >  2:3818899/1‑100 (MQ=255)
                         ctctGGGAAAGCGTTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGtata                                                                        >  1:2738750/1‑100 (MQ=255)
                         ctctGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGtata                                                                        >  2:3402655/1‑100 (MQ=255)
                           ctGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAg                                                                      >  2:3069606/1‑100 (MQ=255)
                           ctGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAg                                                                      <  1:2096531/100‑1 (MQ=255)
                           ctGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAg                                                                      <  2:1644279/100‑1 (MQ=255)
                           ctGGGAAAGCAGTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAg                                                                      <  2:1924462/100‑1 (MQ=255)
                            tGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAgg                                                                     <  1:1697359/100‑1 (MQ=255)
                            tGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAgg                                                                     <  2:2440769/100‑1 (MQ=255)
                            tGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAgg                                                                     <  2:668434/100‑1 (MQ=255)
                            tGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCCGGCGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAgg                                                                     <  2:1026413/100‑1 (MQ=255)
                              ggAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGaa                                                                   >  1:2609641/1‑100 (MQ=255)
                                  aGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAggg                                                               <  2:1288008/100‑1 (MQ=255)
                                   gCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                              <  1:1856392/100‑1 (MQ=255)
                                   gCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                              <  2:3697285/100‑1 (MQ=255)
                                   gCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                              <  1:729798/100‑1 (MQ=255)
                                   gCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                              >  2:4041240/1‑100 (MQ=255)
                                   gCATTGCGCGGGGTCCGGAGCGCAGCAACAATCGGGGGGCAGCAAGGCGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                              <  1:2278751/100‑1 (MQ=21)
                                      ttGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTa                                                            <  1:3108583/99‑1 (MQ=255)
                                      ttGCGCGGGGTCCGGCGCTCATCAACAAGCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTa                                                            <  1:123289/99‑1 (MQ=255)
                                       tGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTatg                                                          <  1:2044506/100‑1 (MQ=255)
                                        gcgcGGGGTCCGGCGCTCATCAAAAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTatga                                                         >  2:2324125/1‑100 (MQ=255)
                                        gcgcGGGGGCGGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTatg                                                          <  2:2462009/99‑1 (MQ=255)
                                         cgcgGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTatgat                                                        <  2:1493738/100‑1 (MQ=255)
                                           cgGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTAtgatga                                                      <  2:1734138/100‑1 (MQ=255)
                                              ggTCCGGCGCTGATCAACAATCTGGGAGTAGCAAGGGGGTGCAAAGGGAACGCCCC‑TCCCGAAGCCGGGGTCTGGTTTAAGGAAAGGGTTatgatg                                                       <  2:2426213/96‑1 (MQ=12)
                                              ggTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGc                                                   <  2:616672/100‑1 (MQ=255)
                                               gTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCt                                                  >  2:3417710/1‑100 (MQ=255)
                                               gTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCt                                                  >  2:3782754/1‑100 (MQ=255)
                                                 ccGGCGCTCATCAACAATCGGGGGGCAGCGAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg                                                >  1:3729996/1‑100 (MQ=255)
                                                 ccGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg                                                >  1:3592746/1‑100 (MQ=255)
                                                 ccGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg                                                <  2:108932/100‑1 (MQ=255)
                                                   ggCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc                                              <  1:341178/100‑1 (MQ=255)
                                                    gcgcTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtca                                             <  1:2898129/100‑1 (MQ=255)
                                                      gcccttcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatc                                           <  1:3805254/95‑1 (MQ=255)
                                                        tcatcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat                                         <  2:3847561/100‑1 (MQ=255)
                                                        tcatcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat                                         >  2:4154006/1‑100 (MQ=255)
                                                            caacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg                                     >  1:809283/1‑100 (MQ=255)
                                                            caacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg                                     >  1:1130222/1‑100 (MQ=255)
                                                             aacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                                    >  1:1393006/1‑100 (MQ=255)
                                                              acaaTCGGGGGGCAGCAAGGGGGTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgt                                   <  1:943118/100‑1 (MQ=255)
                                                              acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGTGGCCTTTTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc                                       <  2:3983324/96‑1 (MQ=255)
                                                              acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgt                                   <  1:100954/100‑1 (MQ=255)
                                                               caaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgtt                                  >  2:1937677/1‑100 (MQ=255)
                                                               caaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgtt                                  >  2:3259084/1‑100 (MQ=255)
                                                                aaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttg                                 <  1:86631/100‑1 (MQ=255)
                                                                 aTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCC‑GCCCGAAGAAGGGGCCTTGTATAAGAAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgt                                >  2:3875534/1‑100 (MQ=255)
                                                                    ggggggCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgtt                             <  1:1046460/100‑1 (MQ=255)
                                                                    ggggggCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgtt                             >  2:2286974/1‑100 (MQ=255)
                                                                    ggggggCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgt                              >  1:3905115/1‑99 (MQ=255)
                                                                     gggggCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg                            <  1:128309/100‑1 (MQ=255)
                                                                      ggggCAGCAAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt                           >  1:3743160/1‑100 (MQ=255)
                                                                         gcagcaAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTAc                        <  1:126275/100‑1 (MQ=255)
                                                                          cagcaAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGTTCCTTGTATAAAGAAAGTGTTATGATGAAGCTCGTCATCATACTGgttgtgtt                             <  1:1348945/94‑1 (MQ=17)
                                                                          cagcaAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACt                       <  2:3369487/100‑1 (MQ=255)
                                                                          cagcaAGGGGCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACt                       <  1:3576308/100‑1 (MQ=255)
                                                                              atggggGTGAGAAGGGAAAGCGCC‑TCCCTAAGAAGGGGCGTGGTATAAGGAAAGGGTTATTATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa                   <  2:3292153/98‑1 (MQ=17)
                                                                              agggggGTGAAACGGGAAAGCCCCTTCCGGAGGAAGGGGCCTTACATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTg                      <  2:1950380/96‑1 (MQ=11)
                                                                                 gggCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGGAAGGGTTATGATGAAGGTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg                 >  2:137632/1‑99 (MQ=255)
                                                                                 gggCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGt                <  2:2989692/100‑1 (MQ=255)
                                                                                 gggCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGGATAAGGAAAAGGTTACGATGAAGCTCGTCAGCATACTGGCTGTGTTGTTACTGTTAAg                 >  2:1563500/1‑99 (MQ=17)
                                                                                  ggCTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGtt               <  2:319123/100‑1 (MQ=255)
                                                                                    cTGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTc             >  1:883955/1‑100 (MQ=255)
                                                                                     tGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTcc            <  1:2981984/100‑1 (MQ=255)
                                                                                     tGAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTcc            <  2:3058990/100‑1 (MQ=255)
                                                                                      gAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGTCTTGTATAAGAAAAGGGATATGATGAATGTCGTCATCATACTGATTGTGTTGTTACTGTTAAGtttac            >  2:301213/1‑97 (MQ=17)
                                                                                      gAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc           >  2:3771908/1‑100 (MQ=255)
                                                                                      gAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc           <  2:2011168/100‑1 (MQ=255)
                                                                                      gAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc           <  2:2059659/100‑1 (MQ=255)
                                                                                      gAAACGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc           >  1:1503016/1‑100 (MQ=255)
                                                                                        aaCGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa         >  1:436819/1‑100 (MQ=255)
                                                                                         aCGGGAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGAc        <  2:1511972/100‑1 (MQ=255)
                                                                                           gggAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACtt      <  1:1334470/100‑1 (MQ=255)
                                                                                            ggAAAACCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTa     <  2:438358/100‑1 (MQ=255)
                                                                                              aaaGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACt   <  1:1427005/100‑1 (MQ=255)
                                                                                              aaaGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACt   <  1:569625/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
GACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCC‑TCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTA  >  NC_000913/3195005‑3195199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A