Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F133 I0 R1	200	171.4	8351354	94.3%	7875326	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,707,989	A→G	75.7%	intergenic (‑284/+627)	dppA ← / ← proK	dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,989	0	A	G	75.7%	372.3 / 116.1	209	intergenic (‑284/+627)	dppA/proK	dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
Reads supporting (aligned to +/- strand): ref base A (28/22); new base G (91/65); total (121/88)
Fisher's exact test for biased strand distribution p-value = 8.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 217 total aligned reads displayed.

ATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAA  >  NC_000913/3707892‑3708087

 tgtgAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGgaa                                                                                                  >  2:265429/1‑99 (MQ=255)

  gtgAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAtt                                                                                                <  1:2983328/100‑1 (MQ=255)

   tgAAAACGTCAATACAGCCAACCGGGAGTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCACAGgaa                                                                                                  >  2:3628426/1‑97 (MQ=255)

     aaaaCGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTtg                                                                                              >  2:2600632/1‑99 (MQ=255)

     aaaaCGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTtgt                                                                                             <  2:3079065/100‑1 (MQ=255)

       aaCGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGc                                                                                           <  1:3297945/100‑1 (MQ=255)

       aaCCTCAATACAGCCAACAGGGATTTATACCAACGGTGAGTATGTAAAAAAAAAAAAAAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTtgtg                                                                                            <  2:146076/99‑1 (MQ=255)

        aCGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCt                                                                                          <  1:151735/100‑1 (MQ=255)

         cGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGCAATTTGTGCTa                                                                                         >  2:2064006/1‑100 (MQ=255)

         cGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTa                                                                                         >  2:1376141/1‑100 (MQ=255)

           tCAATACAGCCAACCGGGATTTACACCATCGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATATGTGCTaat                                                                                       >  2:1059514/1‑98 (MQ=255)

             aatcgaGCCAACCGTGATTTACACCAACGGTGAGAATCCAAACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTcc                                                                                     <  2:2128391/95‑1 (MQ=255)

              aTACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACGTCAAACAGCTATTTGCAGGAGAGGAATTTGTGCTACTCCa                                                                                    >  1:1006457/1‑100 (MQ=255)

               tACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTGCTCCaa                                                                                   >  2:461171/1‑100 (MQ=255)

               tACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCaa                                                                                   >  1:1340333/1‑100 (MQ=255)

                aCAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAAc                                                                                  >  2:386152/1‑100 (MQ=255)

                 cAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAAc                                                                                  <  2:2744003/99‑1 (MQ=255)

                 cAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACa                                                                                 >  1:601604/1‑100 (MQ=255)

                 cAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACa                                                                                 >  2:638426/1‑100 (MQ=255)

                 cAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACa                                                                                 <  1:2593407/100‑1 (MQ=255)

                 cAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACa                                                                                 >  2:4023500/1‑100 (MQ=255)

                 aaGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACACAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACa                                                                                 >  2:1934016/2‑100 (MQ=255)

                  aGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACAt                                                                                >  1:309127/1‑100 (MQ=255)

                   gCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATg                                                                               >  2:356274/1‑100 (MQ=255)

                   gCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATg                                                                               <  1:1588242/100‑1 (MQ=255)

                   gCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATg                                                                               >  1:3451120/1‑100 (MQ=255)

                   gCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATg                                                                               >  1:3509071/1‑100 (MQ=255)

                     cAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGAc                                                                             <  2:1264284/100‑1 (MQ=255)

                     cAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGAc                                                                             <  2:1819520/100‑1 (MQ=255)

                      aaCCGGAATTTACACCAACGGTGAGAATCCACACACAAAGAGTAAAAAAACTTCAAAAAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGAcc                                                                            >  2:2278688/1‑100 (MQ=255)

                        ccGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAg                                                                          >  1:3859204/1‑100 (MQ=255)

                         cGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGa                                                                         <  2:2492037/100‑1 (MQ=255)

                          gggATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGaa                                                                        <  1:1418489/100‑1 (MQ=255)

                          gggATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGaa                                                                        >  1:3914517/1‑100 (MQ=255)

                           ggATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAAc                                                                       <  1:789629/100‑1 (MQ=255)

                                tatacCAACGGTGAGAATCAATAAAAAAAAAAAAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCa                                                                  <  2:1661791/97‑1 (MQ=255)

                                  cacCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGc                                                                >  1:3474572/1‑100 (MQ=255)

                                  cacCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGc                                                                >  1:161535/1‑100 (MQ=255)

                                  cacCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGc                                                                >  1:872331/1‑100 (MQ=255)

                                    ccAACGGTGAGGATCCATACACAAAGATTAGAAAAACGTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCtt                                                              <  2:1907782/100‑1 (MQ=255)

                                    ccAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCtt                                                              >  1:3362359/1‑100 (MQ=255)

                                    ccAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCtt                                                              <  2:3715264/100‑1 (MQ=255)

                                    ccAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCtt                                                              >  1:2631024/1‑100 (MQ=255)

                                    ccAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCtt                                                              >  1:2887759/1‑100 (MQ=255)

                                    ccAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCtt                                                              >  1:3771868/1‑100 (MQ=255)

                                    ccAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCtt                                                              >  1:2324659/1‑100 (MQ=255)

                                      aaCGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTaa                                                            <  1:2440037/100‑1 (MQ=255)

                                       aCGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAat                                                           <  2:540021/100‑1 (MQ=255)

                                       aCGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAat                                                           <  1:3692040/100‑1 (MQ=255)

                                         ggTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAatat                                                         >  1:983319/1‑100 (MQ=255)

                                          gTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATAtt                                                        >  1:3155666/1‑100 (MQ=255)

                                            gagaATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTa                                                      >  1:733598/1‑100 (MQ=255)

                                            gagaATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTa                                                      <  2:1340325/100‑1 (MQ=255)

                                              gaATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGc                                                    <  2:2209854/100‑1 (MQ=255)

                                              gaATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGc                                                    <  1:4032579/100‑1 (MQ=255)

                                              gaATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGc                                                    >  2:3383534/1‑100 (MQ=255)

                                                aTCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCaa                                                  >  1:1100553/1‑100 (MQ=255)

                                                 tCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAAc                                                 <  1:2635130/100‑1 (MQ=255)

                                                   cacacacaAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACAt                                               >  1:2872454/1‑100 (MQ=255)

                                                   cacacacaAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACAt                                               >  1:3605455/1‑100 (MQ=255)

                                                       cacaAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATATGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACAt                                               >  2:2945714/1‑96 (MQ=255)

                                                        acaAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGt                                          <  2:872326/100‑1 (MQ=255)

                                                         ccaaGATTCGAAAAACTTCAAACAGCTATTTGCAGAAGAGGAATTTGTGCTACTCCAACATGCCCAGCACAATCAGCTTAATATTTAGCACAATTTgtt                                          >  2:1697038/3‑97 (MQ=255)

                                                         caAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTg                                         <  2:2850339/100‑1 (MQ=255)

                                                          aaaGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGa                                        >  2:3901021/1‑100 (MQ=255)

                                                           aaGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGAt                                       >  1:908476/1‑100 (MQ=255)

                                                              aTTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTaa                                    >  1:7443/1‑100 (MQ=255)

                                                              aTTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTaa                                    <  2:3116349/100‑1 (MQ=255)

                                                               ttAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAg                                   >  2:3497670/1‑100 (MQ=255)

                                                               ttAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAg                                   >  1:2236547/1‑100 (MQ=255)

                                                                tAAAAAAACTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCAAACATGAACAGAACAATCAGCTTAATATATAGCAATATTTGGTGATTAAg                                   >  2:3790562/1‑99 (MQ=255)

                                                                tAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGt                                  <  1:2257969/100‑1 (MQ=255)

                                                                   aaaaaCTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGGACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGtttt                               >  2:2932888/1‑100 (MQ=255)

                                                                    aaaaCTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTa                              >  2:1740452/1‑100 (MQ=255)

                                                                      aaCTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATg                            <  1:1745852/100‑1 (MQ=255)

                                                                      aaCTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATg                            <  1:3040328/100‑1 (MQ=255)

                                                                      aaCGTCAAACAGCTATAGGCAGCAGATGAAGTTGTGCTACTCCAACATGCCCAGAACAAGCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTAt                             <  2:3748652/99‑1 (MQ=255)

                                                                       aCTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATg                            >  2:3996988/1‑99 (MQ=255)

                                                                        cTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTCCTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCa                          <  2:4094291/100‑1 (MQ=255)

                                                                        cTTCAAACAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCa                          >  1:4101765/1‑100 (MQ=255)

                                                                            aaaCAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTa                      >  1:2143281/1‑100 (MQ=255)

                                                                            aaaCAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTa                      >  2:3298681/1‑100 (MQ=255)

                                                                            aaaCAGCTATTTGCAGCAGAGCAATTTGTCCTACTCCAACATTACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCAttt                       >  2:818207/1‑99 (MQ=255)

                                                                             aaCAGCTATTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTaa                     >  1:2049274/1‑100 (MQ=255)

                                                                             aaCAGCTATTTGAAGCAGAGGAATTTGTGCTACTCGAACATGAGGAGAACAATGAGCTTAATATTTCACAAAATTTGGTGATTAAGTTTTATGCATTTa                      >  2:1033425/1‑99 (MQ=255)

                                                                              aCAGCTATTTGCAGCAGAGAAATTTGTGCTACTACAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAAt                    <  2:3337908/100‑1 (MQ=255)

                                                                                 gCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGaa                 >  1:1290353/1‑100 (MQ=255)

                                                                                    aTTTGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGaaaaa              >  2:665328/1‑100 (MQ=255)

                                                                                       tGCAGCAGAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAAt           >  2:3442668/1‑100 (MQ=255)

                                                                                         cagcagAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCt         >  2:1137069/1‑100 (MQ=255)

                                                                                          agcagAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTg        >  1:265890/1‑100 (MQ=255)

                                                                                          agcagAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTg        <  1:4023530/100‑1 (MQ=255)

                                                                                           gcagAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCtcc       >  1:423355/1‑98 (MQ=255)

                                                                                           gcagAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGa       >  1:2396300/1‑100 (MQ=255)

                                                                                           gcagAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGa       >  2:2410489/1‑100 (MQ=255)

                                                                                           gcagAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGa       >  2:117818/1‑100 (MQ=255)

                                                                                            cagAGGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTg        >  2:1296939/1‑98 (MQ=255)

                                                                                            cagAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAg      <  1:2769783/100‑1 (MQ=255)

                                                                                             agagGAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAgg     <  1:245659/100‑1 (MQ=255)

                                                                                              gaggAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGa    >  1:1975169/1‑100 (MQ=255)

ATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAA  >  NC_000913/3707892‑3708087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A