Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
339,856 |
T→C |
intergenic (+113/‑309) |
yahG → / → yahI |
DUF1116 family protein/carbamate kinase‑like protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 339,856 | 0 | T | C | 92.7%
| 115.0
/ 1.1
| 42 | intergenic (+113/‑309) | yahG/yahI | DUF1116 family protein/carbamate kinase‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (16/22); total (17/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01 |
ATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGC > NC_000913/339762‑339951
|
aTGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACgcggc < 2:324502/100‑1 (MQ=255)
gAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGcc < 1:953578/100‑1 (MQ=255)
ccgatcTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCt < 2:568062/95‑1 (MQ=255)
aaCGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGTCGGACGCGGCGTGAACGCCt < 2:794355/100‑1 (MQ=255)
aaCGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCt < 1:932945/100‑1 (MQ=255)
aaCGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCt < 2:280999/100‑1 (MQ=255)
cTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATcc < 1:636934/100‑1 (MQ=255)
tATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCgg > 1:97816/1‑100 (MQ=255)
aTCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTCCCGGACGCGGCGTGAACGCCTTATcc > 2:87650/1‑97 (MQ=255)
cttACGAATAGCGCAAGAATCTGTAGGCCTGATAAGTGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGa < 2:337917/98‑1 (MQ=11)
tACGAATGGCGCAAGAATCTTTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCAGCGTGAACGCCTTATCCGGCCTACGa < 2:337865/98‑1 (MQ=255)
tACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAAt < 1:1297363/100‑1 (MQ=255)
aCGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATg < 1:162027/100‑1 (MQ=255)
aaTGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGgcg > 2:1264721/1‑100 (MQ=255)
ggCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGgcg > 2:1076892/1‑97 (MQ=38)
aGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCGAGAATCt > 2:340387/1‑100 (MQ=9)
aTCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTa > 1:698617/1‑100 (MQ=255)
tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGc < 1:566659/100‑1 (MQ=255)
tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGAAGGc > 2:278964/1‑100 (MQ=255)
tAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCt < 1:637670/100‑1 (MQ=255)
ggCCGGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGa < 2:903292/100‑1 (MQ=255)
ccTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATa < 1:558139/100‑1 (MQ=255)
gATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCGAGAATCTGAAGGCCGGCTAAGc > 2:444842/1‑100 (MQ=11)
gATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAg > 1:1050699/1‑99 (MQ=255)
naaGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGt > 1:385087/2‑100 (MQ=255)
gCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAgc > 2:686355/1‑100 (MQ=255)
gCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAgc < 1:1264732/100‑1 (MQ=255)
gTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAgcgc > 1:1088762/1‑100 (MQ=255)
gTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAgcgc < 1:779121/100‑1 (MQ=255)
cgcATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCa > 1:964991/1‑100 (MQ=255)
gcATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAg < 1:87652/100‑1 (MQ=255)
ggCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAttt < 1:1228367/100‑1 (MQ=255)
aTTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGcc < 1:990928/100‑1 (MQ=255)
gTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTGGGCCTGTTAACCGTAGGGCATCAGGCATTTGccacc > 2:1133486/1‑99 (MQ=14)
tCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCcaccac < 1:686359/100‑1 (MQ=255)
cACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACt < 1:104687/100‑1 (MQ=255)
cATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGcc > 1:1186182/1‑100 (MQ=255)
aTTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGccacc > 2:754308/1‑93 (MQ=25)
tGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCg < 2:875027/98‑1 (MQ=255)
ggCCGGATAAGGCGTTCACGCCGCGTCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCAGGa > 2:1119951/2‑100 (MQ=11)
gCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGACATTTGCCACCACTGCCGGAt < 1:1195970/100‑1 (MQ=255)
cGGAGGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATgc < 2:1203810/100‑1 (MQ=255)
|
ATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGC > NC_000913/339762‑339951
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
