Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,306,399 |
C→T |
W119* (TGG→TAG) |
mqo ← |
malate dehydrogenase, FAD/NAD(P)‑binding domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,306,399 | 0 | C | T | 96.3%
| 175.8
/ ‑1.7
| 54 | W119* (TGG→TAG) | mqo | malate dehydrogenase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (26/26); total (28/26) |
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 |
ACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTC > NC_000913/2306306‑2306493
|
aCGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACt > 2:526675/1‑100 (MQ=255)
ccTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGa > 1:913285/1‑100 (MQ=255)
cTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGaa > 1:344752/1‑100 (MQ=255)
gCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATc < 1:295273/100‑1 (MQ=255)
ccccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCt < 1:132907/100‑1 (MQ=255)
ccccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCt > 2:401496/1‑100 (MQ=255)
ccccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCt > 1:940662/1‑100 (MQ=255)
cccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTg < 2:923725/100‑1 (MQ=255)
cccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTg < 2:273741/100‑1 (MQ=255)
cccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTg > 1:290107/1‑100 (MQ=255)
ccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGa > 2:69141/1‑100 (MQ=255)
aGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGaaa < 1:547377/100‑1 (MQ=255)
cAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGc > 2:890950/1‑100 (MQ=255)
aaGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCtt > 1:1186954/1‑100 (MQ=255)
aaGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCtt > 1:526441/1‑100 (MQ=255)
gCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCg > 2:127502/1‑100 (MQ=255)
cTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGt > 1:81596/1‑100 (MQ=255)
cTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGt < 1:642430/100‑1 (MQ=255)
tCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGtt > 1:170969/1‑100 (MQ=255)
tCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGtt > 1:246281/1‑100 (MQ=255)
cATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTa > 2:718735/1‑100 (MQ=255)
atGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATg < 2:445894/100‑1 (MQ=255)
tGCGGAACGGTATTGATAAATGAACGCGGCGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATgg < 2:787086/100‑1 (MQ=255)
cGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCg < 2:58183/100‑1 (MQ=255)
cGGAACGGTATTGATAAATGAACGAAGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTCGAAGTGGCGGGAAATCTGAAATGCTTCGTTAATgg > 2:80565/1‑98 (MQ=255)
aaCGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACt < 1:69142/100‑1 (MQ=255)
aaCGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACt < 1:436507/100‑1 (MQ=255)
aTTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCtttt < 1:890957/100‑1 (MQ=255)
aTTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCtttt > 2:314715/1‑100 (MQ=255)
aTTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCtttt > 2:81238/1‑100 (MQ=255)
gATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCa < 1:498106/100‑1 (MQ=255)
gATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCa < 1:178433/100‑1 (MQ=255)
tAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAAt < 1:268410/100‑1 (MQ=255)
tAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAAt > 1:664189/1‑100 (MQ=255)
aaaTGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATa > 1:1048125/1‑100 (MQ=255)
aaCGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGCAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGAt > 1:1290218/1‑100 (MQ=255)
aCGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCg < 1:750972/100‑1 (MQ=255)
cGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGTGAAATCTGAAATGCTTCGTGAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCgg > 2:709885/1‑100 (MQ=255)
cGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCATTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCgg > 2:184391/1‑100 (MQ=255)
gcagcaCGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGc < 1:785499/100‑1 (MQ=255)
agcaCGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGt < 2:253043/100‑1 (MQ=255)
ccgcGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCg < 2:1141693/100‑1 (MQ=255)
gcgcTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCggg < 2:1179402/100‑1 (MQ=255)
gcgcTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCggg < 1:640074/100‑1 (MQ=255)
cgcTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCgggg > 1:547382/1‑100 (MQ=255)
cTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgtg < 1:49319/100‑1 (MQ=255)
cTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgtg > 2:766128/1‑100 (MQ=255)
tCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgtgt < 1:1196479/100‑1 (MQ=255)
cAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgtgt > 2:102638/1‑99 (MQ=255)
aCCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagt > 2:578250/1‑100 (MQ=255)
tGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagttca < 2:919135/100‑1 (MQ=255)
tGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagttca < 2:940654/100‑1 (MQ=255)
gcgcCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagttcagttc < 2:913277/100‑1 (MQ=255)
gcgcCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagttcagtt > 2:834749/1‑99 (MQ=255)
|
ACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTC > NC_000913/2306306‑2306493
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
