Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,461,483 |
G→T |
A60S (GCA→TCA) |
fadL → |
long‑chain fatty acid outer membrane transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,461,483 | 0 | G | T | 95.2%
| 207.5
/ 0.8
| 62 | A60S (GCA→TCA) | fadL | long‑chain fatty acid outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base T (23/36); total (25/37) |
| Fisher's exact test for biased strand distribution p-value = 5.60e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 |
TTTCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTC > NC_000913/2461387‑2461576
|
tttCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCAt > 1:617046/1‑100 (MQ=255)
tttCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCAt > 1:344419/1‑100 (MQ=255)
tCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTg > 1:920018/1‑100 (MQ=255)
gTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGAtt > 1:694869/1‑100 (MQ=255)
gTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGAtt < 2:838951/100‑1 (MQ=255)
ttAAACGAATTTTCTTTCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTa < 2:761611/100‑1 (MQ=255)
ttAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTa < 2:214318/100‑1 (MQ=255)
aaaCGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTAc < 2:128741/99‑1 (MQ=255)
aCGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTAt < 2:514396/100‑1 (MQ=255)
cGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATg > 1:1151555/1‑100 (MQ=255)
aaTTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGtt < 1:148962/100‑1 (MQ=255)
aTTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGACGCAATTGCCGATGATGCAAGTAACGGCAGCCGTAACCCCGCATTGATTACTATGtt > 2:713003/1‑99 (MQ=255)
ttttCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTg < 2:730820/100‑1 (MQ=255)
ttttCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTg < 1:1117457/100‑1 (MQ=255)
ttcttcCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGAc < 1:577979/100‑1 (MQ=255)
tcttcCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATCTTTGAcc < 1:684105/100‑1 (MQ=255)
cttcCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCg > 1:739177/1‑100 (MQ=255)
tctGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTTACCGCCCGa > 2:326476/1‑100 (MQ=255)
ctGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGAc > 2:203937/1‑100 (MQ=255)
ctGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGAc < 2:532979/100‑1 (MQ=255)
gCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACAtt > 2:1068614/1‑100 (MQ=255)
ccTGGGCCGGGCTTTATAAGGAGCAGGCGCAATTACCGATGATGCAGGTAATGTGAGGCTGAAGCTCTCATTGGTTACTATTGTTGACCGCCCTACAtgt > 2:433611/1‑98 (MQ=255)
gggCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCt < 1:583213/100‑1 (MQ=255)
ggCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTg > 1:151730/1‑100 (MQ=255)
gCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGc > 2:59245/1‑100 (MQ=255)
tATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCgg > 1:178741/1‑100 (MQ=255)
aTTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTATCGTCAGCCGTAACCCCTCATTGATTACTATGTTTGTCCGCCCGACATTTTCTGCGGGTGCGGt < 1:915526/100‑1 (MQ=255)
aTTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAGCGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGt < 1:915524/100‑1 (MQ=255)
aTTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGt > 1:132867/1‑100 (MQ=255)
ttCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGtt < 2:617042/100‑1 (MQ=255)
ttCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGtt < 2:698219/100‑1 (MQ=255)
ttCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGtt > 2:941108/1‑100 (MQ=255)
cAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTta < 1:975957/100‑1 (MQ=255)
cAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTta < 1:379561/100‑1 (MQ=255)
gggAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAAACCCTCATTGATTACTATGTTTGAGCGCCCGACATTTTCTGCGGGTGCGGTTTatat > 2:767949/1‑100 (MQ=255)
ggCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGAcc > 1:1155035/1‑100 (MQ=255)
gcgcAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGAccc < 1:554271/100‑1 (MQ=255)
cgcAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCg < 2:1155024/100‑1 (MQ=255)
gcAATTGCCGATGATGCAGGTACCGTCAGCCTTAACCACTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCgg < 2:877183/100‑1 (MQ=255)
gcAATTGCCGATGATGCAGGTAACGTCCGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCgg < 2:231241/100‑1 (MQ=255)
cAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGa > 1:440493/1‑100 (MQ=255)
aTTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATg < 2:920010/100‑1 (MQ=255)
gCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTa > 1:672264/1‑99 (MQ=255)
cGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAat < 1:1198631/100‑1 (MQ=255)
gatgatGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAata < 1:260147/100‑1 (MQ=255)
atgatgCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAatat < 2:178739/100‑1 (MQ=255)
tgatgCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATc < 2:1040653/100‑1 (MQ=255)
tgCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGc < 2:263420/100‑1 (MQ=255)
aGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGa < 1:924129/100‑1 (MQ=255)
gTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAAc < 1:368595/100‑1 (MQ=255)
tAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACg < 1:1055569/100‑1 (MQ=255)
gTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGtctc < 2:445807/100‑1 (MQ=255)
gTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGtctc > 1:852188/1‑100 (MQ=255)
gTCACCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGACCGtctc > 2:1016534/1‑100 (MQ=255)
tCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTcc > 2:818205/1‑100 (MQ=255)
tCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTcc < 1:1116495/100‑1 (MQ=255)
aGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCAt > 2:432052/1‑100 (MQ=255)
gCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTTCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATc < 1:499554/100‑1 (MQ=255)
gCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATc > 2:159415/1‑100 (MQ=255)
gTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTgg < 1:60829/100‑1 (MQ=255)
aaCCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTc < 1:1246847/100‑1 (MQ=255)
aaCCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTc < 2:621444/100‑1 (MQ=255)
|
TTTCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTC > NC_000913/2461387‑2461576
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
