Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,118,566 |
C→T |
A183T (GCG→ACG) |
sslE ← |
putative secreted and surface‑associated lipoprotein mucinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,118,566 | 0 | C | T | 95.2%
| 199.1
/ 0.4
| 63 | A183T (GCG→ACG) | sslE | putative secreted and surface‑associated lipoprotein mucinase |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (25/35); total (26/37) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 |
ACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGAC > NC_000913/3118470‑3118660
|
aCTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCgg < 1:433399/100‑1 (MQ=255)
aCTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCgg > 1:1176482/1‑100 (MQ=255)
ctctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGa > 1:562887/1‑100 (MQ=255)
ctctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGa > 1:679296/1‑100 (MQ=255)
ctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACa < 1:499936/100‑1 (MQ=255)
ctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACa < 1:170962/100‑1 (MQ=255)
ttCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAg < 1:957039/100‑1 (MQ=255)
tCCGGTGCCAGATCTATTTGCTTATACAGCGAGTCTACGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGc < 2:1052650/100‑1 (MQ=255)
cGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTg < 1:418799/100‑1 (MQ=255)
acgctcttccGATCTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGtt < 2:650518/91‑1 (MQ=255)
ccAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACt > 1:54289/1‑100 (MQ=255)
cAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTCCTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTg < 2:170162/100‑1 (MQ=255)
cAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTg > 2:490658/1‑100 (MQ=255)
gATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGa < 2:150584/100‑1 (MQ=255)
tCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACg < 1:346471/100‑1 (MQ=255)
ctaTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGt > 1:883310/3‑100 (MQ=255)
gATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGtt > 2:367830/1‑100 (MQ=255)
tttGC‑TATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTAcc < 2:212797/100‑1 (MQ=255)
taCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGaaa < 2:1011634/100‑1 (MQ=255)
taCAGCGAGTCGAAGCGTTTACTCTCAATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGaaa > 1:795114/1‑100 (MQ=255)
taCAGCGAGTCGAAGCGTTTACTCTCAATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGaaa > 1:795314/1‑100 (MQ=255)
acggCGAGTCGAAGCGTTTACTCTCGCTCACCGAGGAGAACGTCAGAACAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAAc < 2:1087008/97‑1 (MQ=255)
aaaGCGAGTAGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAATCATGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAAc < 2:1086990/98‑1 (MQ=255)
cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCa < 1:1049741/100‑1 (MQ=255)
aGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCAtt < 2:562883/100‑1 (MQ=255)
tCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGc < 2:883303/100‑1 (MQ=255)
cGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCt < 1:315416/100‑1 (MQ=255)
aaGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCttt < 1:125173/100‑1 (MQ=255)
tACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCACTGCTTTTCCTg > 2:603668/1‑98 (MQ=255)
tACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTc > 1:1304515/1‑100 (MQ=255)
ctcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCg > 1:802373/1‑100 (MQ=255)
tcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCgg > 2:992301/1‑100 (MQ=255)
tcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCgg < 2:991539/100‑1 (MQ=255)
tcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCgg < 1:67304/100‑1 (MQ=255)
gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAg > 1:1217701/1‑100 (MQ=255)
aTCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGc < 1:1146239/100‑1 (MQ=255)
cGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGcc < 2:463282/100‑1 (MQ=255)
gaggagAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGGCGTTAGCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCa < 1:367833/100‑1 (MQ=255)
gaggagAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCa > 2:1274233/1‑100 (MQ=255)
ggagAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGt > 1:416656/1‑100 (MQ=255)
ggagAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGt > 2:907403/1‑100 (MQ=255)
gaACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACTAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCt > 2:1021649/1‑100 (MQ=255)
aaCGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCtt > 1:459666/1‑100 (MQ=255)
cGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgc < 1:907410/100‑1 (MQ=255)
gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcn < 1:59114/100‑2 (MQ=255)
gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg > 2:1285807/1‑100 (MQ=255)
gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg > 1:756461/1‑100 (MQ=255)
gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg < 1:423513/100‑1 (MQ=255)
gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg < 2:679292/100‑1 (MQ=255)
tCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc > 2:656434/1‑100 (MQ=255)
cAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc < 2:1025509/99‑1 (MQ=255)
cAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGcgcg < 1:634538/100‑1 (MQ=255)
cAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGcgcg < 1:393354/100‑1 (MQ=255)
aGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGt > 2:1141243/1‑100 (MQ=255)
gACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTc > 1:640902/1‑100 (MQ=255)
aCAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTcc < 2:309854/100‑1 (MQ=255)
cAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCt < 2:1052177/100‑1 (MQ=255)
tGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGc > 1:837698/1‑100 (MQ=255)
ttCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTa < 2:456131/100‑1 (MQ=255)
cTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTaaa > 2:357564/1‑100 (MQ=255)
tGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAAc < 1:1169286/100‑1 (MQ=255)
aTTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGac < 1:470273/100‑1 (MQ=255)
ttCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACg < 2:814936/97‑1 (MQ=255)
|
ACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGAC > NC_000913/3118470‑3118660
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
