Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,244,928 |
T→C |
intergenic (‑187/‑296) |
uxaC ← / → exuT |
uronate isomerase/hexuronate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,244,928 | 0 | T | C | 98.3%
| 207.6
/ ‑4.2
| 60 | intergenic (‑187/‑296) | uxaC/exuT | uronate isomerase/hexuronate transporter |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (30/29); total (31/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 |
CGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAC > NC_000913/3244833‑3245023
|
cGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATg > 2:504068/1‑100 (MQ=255)
gTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGt > 2:465251/1‑100 (MQ=255)
tGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTc < 1:1118309/100‑1 (MQ=255)
gTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCt > 2:257601/1‑100 (MQ=255)
gTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCt > 1:302561/1‑100 (MQ=255)
gATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACaa > 1:1069961/1‑100 (MQ=255)
gATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACaa > 2:177430/1‑100 (MQ=255)
tAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTc > 1:613296/1‑100 (MQ=255)
aaaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTcc > 2:1244109/1‑100 (MQ=255)
aaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTccc < 1:670011/100‑1 (MQ=255)
gTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCcaca > 1:510505/1‑100 (MQ=255)
tAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTtgt > 1:246544/1‑100 (MQ=255)
tAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTtgt > 2:370028/1‑100 (MQ=255)
aaaaaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTtgtg < 2:1010492/100‑1 (MQ=255)
aaaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGAt < 1:148158/100‑1 (MQ=255)
aaaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGAt > 1:1206056/1‑100 (MQ=255)
aaaaTTACTCTCAAAGTGGTAAATCTAGCTGCAGGCCGCGCCAGTACTGGATTTGCTGTCGTCAGGCACTGTCCCTACAAACATTCCCACATTTGTGAt > 2:756306/1‑99 (MQ=255)
aaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGc < 1:170913/100‑1 (MQ=255)
aaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGc < 2:246541/100‑1 (MQ=255)
aTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGct < 1:695962/100‑1 (MQ=255)
aCTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc > 2:900210/1‑100 (MQ=255)
aCTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc > 2:336446/1‑100 (MQ=255)
aCTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc > 1:792932/1‑100 (MQ=255)
tctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAc < 2:1039832/100‑1 (MQ=255)
ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc < 1:370031/100‑1 (MQ=255)
ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc > 1:1010837/1‑100 (MQ=255)
ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATCTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc > 2:245799/1‑100 (MQ=255)
ctcAAAGTGGTAAATCTCGCAGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc > 2:800155/1‑100 (MQ=255)
tcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCt > 1:441788/1‑100 (MQ=255)
tcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCt > 2:1231155/1‑100 (MQ=255)
cAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCtt < 1:142088/100‑1 (MQ=255)
tAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGtt > 1:1051/1‑100 (MQ=255)
acaTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTg < 2:792927/98‑1 (MQ=255)
aaaTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTg < 2:527571/100‑1 (MQ=255)
aTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTa < 2:168391/100‑1 (MQ=255)
tctcGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTAt < 1:224666/100‑1 (MQ=255)
ctcGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATg > 2:111633/1‑100 (MQ=255)
cTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACaa > 2:680484/1‑100 (MQ=255)
gCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGt < 2:1183145/100‑1 (MQ=255)
ggCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTAt < 2:231673/100‑1 (MQ=255)
ggCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTAt > 2:235274/1‑100 (MQ=255)
gCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATc < 1:32726/100‑1 (MQ=255)
cgcgCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt > 1:1274313/1‑100 (MQ=255)
cgcgCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt < 2:439359/100‑1 (MQ=255)
cgcgCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt < 2:439820/100‑1 (MQ=255)
ccAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTg < 1:71530/100‑1 (MQ=255)
ccAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTg < 1:800160/100‑1 (MQ=255)
aGTACTTCCCTTGCTGTCGTAAGGCAATGTCCTTATGAAGATTTGCTAATTTGTGATGGCTCTCCATTTTTAATCTTTTATGAGATGTTATCtttacgcc < 2:1089907/100‑6 (MQ=255)
aGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGcc > 1:869534/1‑100 (MQ=255)
tACTGGACTAGCTGCCGTCAGGCAATGTCGCTACAAATATTCCCACATTTGTTATAGCGCTCACCTTTTAAAGTTGTGTTACAAGTTATCGTTcctcag < 2:530689/99‑6 (MQ=255)
cattGCTGTCGTCGGGTAATGTCCCTACAAATATTCCCACATATGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCaaa > 1:882204/3‑100 (MQ=255)
tGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCa < 1:331283/100‑1 (MQ=255)
tGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCa < 1:1244120/100‑1 (MQ=255)
gCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAATTcct > 1:62927/1‑98 (MQ=255)
tGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATaa > 1:352267/1‑100 (MQ=255)
gtcgtcAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAg < 2:195421/100‑1 (MQ=255)
gtcgtcAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAAATAAAAAAAAAATAATATTTCTGCCGTCGCAAATCATAAg < 2:613292/100‑1 (MQ=255)
gtcAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCg > 1:366963/1‑100 (MQ=255)
gtcAGGCAATGTCCCTACAAATATCCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGt < 1:111635/98‑1 (MQ=255)
cAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAc < 1:1015786/100‑1 (MQ=255)
|
CGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAC > NC_000913/3244833‑3245023
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
