Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F56 I1 R1	165	53.8	2611523	96.4%	2517508	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,686,929	G→T	Q749K (CAG→AAG)	bcsC ←	cellulose synthase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,686,929	0	G	T	100.0%	162.6 / NA	46	Q749K (CAG→AAG)	bcsC	cellulose synthase subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (22/24); total (22/24)

GGCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTC  >  NC_000913/3686833‑3687020

ggCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCgg                                                                                          >  2:813842/1‑100 (MQ=255)

ggCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCgg                                                                                          >  2:122671/1‑100 (MQ=255)

 gCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGa                                                                                         >  1:301708/1‑100 (MQ=255)

   gATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGAcg                                                                                       <  2:611229/100‑1 (MQ=255)

       gagGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGcgtc                                                                                   >  1:763141/1‑100 (MQ=255)

         ggTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGcgtcgt                                                                                 <  1:565394/100‑1 (MQ=255)

           tCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAg                                                                               <  1:715551/100‑1 (MQ=255)

            ccgccgCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGt                                                                              <  1:1247973/100‑1 (MQ=255)

            ccgccgCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGt                                                                              >  1:356136/1‑100 (MQ=255)

             cgccgcATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTc                                                                             <  1:1166757/100‑1 (MQ=255)

             cgccgcATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTc                                                                             >  1:17650/1‑100 (MQ=255)

              gccgcATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTca                                                                            >  2:153005/1‑100 (MQ=255)

                 gcATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCacac                                                                         >  2:597928/1‑100 (MQ=255)

                        tGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGc                                                                  <  1:142979/100‑1 (MQ=255)

                         gcgcACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGc                                                                  <  1:151569/99‑1 (MQ=255)

                                  aCGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCg                                                        <  2:15220/100‑1 (MQ=255)

                                    gTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTc                                                      >  2:713994/1‑100 (MQ=255)

                                    gTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTc                                                      <  1:724932/100‑1 (MQ=255)

                                    gTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTc                                                      <  1:756673/100‑1 (MQ=255)

                                      tctaGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCtt                                                    <  2:983102/97‑1 (MQ=255)

                                       tCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCttt                                                   >  1:821016/1‑100 (MQ=255)

                                         aGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCttaga                                                 >  1:983110/1‑97 (MQ=255)

                                         aGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGt                                                 >  2:599534/1‑100 (MQ=255)

                                          gCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTa                                                <  1:122673/100‑1 (MQ=255)

                                              gTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGtt                                            >  1:862640/1‑100 (MQ=255)

                                                 aTCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTcc                                         <  2:1238973/100‑1 (MQ=255)

                                                 aTCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTAGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTcc                                         <  2:49046/100‑1 (MQ=255)

                                                   cTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAg                                       >  2:781784/1‑100 (MQ=255)

                                                     ttCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAgcg                                     <  2:426426/100‑1 (MQ=255)

                                                       ctcGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGcc                                   <  1:173411/100‑1 (MQ=255)

                                                       ctcGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGcc                                   >  1:63656/1‑100 (MQ=255)

                                                         cgtcgtTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTg                                 >  2:1262647/1‑100 (MQ=255)

                                                           tcgtTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCg                               <  1:742433/100‑1 (MQ=255)

                                                              ttACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTg                            <  1:402365/100‑1 (MQ=255)

                                                               tACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTgg                           >  1:1122365/1‑100 (MQ=255)

                                                                 cGGGTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGAt                         >  1:461656/1‑100 (MQ=255)

                                                                  gggTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATc                        <  2:17650/100‑1 (MQ=255)

                                                                   ggTCAGTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcg                       <  1:597932/100‑1 (MQ=255)

                                                                   ggTCAGTCGGGTAAAGGTGTAGTTATCATTCGGACGCGTCGTAGTCACACCGGATGCGACCATAGCGACTTTGTAGGTTTCCAGCGCCTGCGTTGGATcg                       >  2:589013/1‑100 (MQ=255)

                                                                        gTCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcgcccgc                  >  1:135568/1‑100 (MQ=255)

                                                                         tCGGGTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATcgcccgcc                 <  1:778260/100‑1 (MQ=255)

                                                                             gTAAAGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTgcg             <  1:970857/100‑1 (MQ=255)

                                                                                 aGGTGTCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTc         >  2:1230373/1‑100 (MQ=255)

                                                                                   gtgtCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCaa       <  2:465764/100‑1 (MQ=255)

                                                                                    tgtCGTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCaaa      >  2:196569/1‑100 (MQ=255)

                                                                                        gTTATCCTTCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGGCCGCCTGCGCTTCAAACTTc  <  2:796955/100‑1 (MQ=255)

GGCGATAGAGGTCCGCCGCATCGCTGCGCACGCCACGTTTCAGCCAGTCATCTTTCTCGTCGTTACGGGTCAGTCGGGTAAAGGTGTCGTTATCCTGCGGACGCGTCGTAGTCACACCGGATGCGACCATGGCGTCTTTGTAGGTTTCCAGCGCCTGCGTTGGATCGCCCGCCTGCGCTTCAAACTTC  >  NC_000913/3686833‑3687020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A