Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,168,540 |
T→A |
intergenic (+93/‑101) |
gltT → / → rrlB |
tRNA‑Glu/23S ribosomal RNA of rrnB operon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,168,540 | 0 | T | A | 89.8%
| 129.1
/ 11.5
| 59 | intergenic (+93/‑101) | gltT/rrlB | tRNA‑Glu/23S ribosomal RNA of rrnB operon |
| Reads supporting (aligned to +/- strand): ref base T (4/2); new base A (25/28); total (29/30) |
| Fisher's exact test for biased strand distribution p-value = 4.24e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01 |
CGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTG > NC_000913/4168443‑4168639
|
cGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACa < 1:927028/100‑1 (MQ=255)
gCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACaa > 2:1140207/1‑100 (MQ=255)
ccACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAg > 1:1068389/1‑100 (MQ=255)
ccACTTGCTGGCTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAg < 1:988310/100‑1 (MQ=255)
aCTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCt < 2:650136/100‑1 (MQ=255)
ggTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAAtt > 1:1069321/1‑100 (MQ=255)
ggTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATATTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAAtt > 1:108313/1‑100 (MQ=255)
ggttGTGGGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAAt < 1:101952/96‑1 (MQ=255)
gTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTg > 1:1245019/1‑100 (MQ=255)
gTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTg < 2:79482/100‑1 (MQ=255)
ttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaa < 2:961551/100‑1 (MQ=255)
ttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGa < 1:185843/99‑1 (MQ=255)
gtgagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAac < 1:547963/100‑1 (MQ=255)
tgagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAaca > 2:623072/1‑100 (MQ=255)
gagtgaAAGTCGCCGACCTTAATATCTCAAAACTTATCTGCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAacac < 2:1215080/100‑1 (MQ=255)
agtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACt > 2:67508/1‑100 (MQ=255)
tgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGa > 1:1246094/1‑100 (MQ=255)
tgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCACTTTAAGAATCTGGAACAAGCTGAAAATTGAAACACTGa > 2:915912/1‑100 (MQ=255)
gaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaa < 1:490604/100‑1 (MQ=255)
aaGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaaca > 2:999275/1‑100 (MQ=255)
ccGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACgaga < 2:1196205/100‑1 (MQ=25)
aCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAgtt > 2:831437/1‑100 (MQ=25)
ccTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAGAgttg > 2:426079/1‑100 (MQ=33)
ttAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAGAgttgtt < 2:1068379/100‑1 (MQ=32)
aaTATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCg < 2:146614/100‑1 (MQ=18)
atatCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGt < 1:114881/100‑1 (MQ=18)
tatCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTg < 2:621763/100‑1 (MQ=18)
atCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGa > 1:132812/1‑100 (MQ=17)
tctcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAg < 1:789823/100‑1 (MQ=18)
aaCTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGtctctc < 1:1140217/100‑1 (MQ=18)
aaCTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGtctctc < 2:1341/100‑1 (MQ=18)
aCTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCa < 1:397002/100‑1 (MQ=18)
aTCTTCGGGTGATGTTTGAGATATTTGATCTTTAAAAATCTGGTAGAAGCTGAAAATTTAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAAtt < 2:750993/100‑1 (MQ=11)
cTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAAtttt > 2:949897/1‑100 (MQ=18)
tCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCg < 1:1093442/100‑1 (MQ=18)
tCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCg < 2:971548/100‑1 (MQ=18)
gggTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCa < 1:318209/100‑1 (MQ=18)
ttGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatga > 1:1239219/1‑100 (MQ=18)
ttGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGATGTTCGTAAATCTCTCAAATTTTCGCAACACgatga > 2:273932/1‑100 (MQ=11)
gagaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGatgatg < 1:1185543/100‑1 (MQ=17)
agaTATTTGCTCTTTAAAAATCTGGAACAAGCTAAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGAtgatga > 1:287475/1‑100 (MQ=255)
gaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGaa < 2:1069311/100‑1 (MQ=18)
atatTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAAt < 2:1245008/100‑1 (MQ=18)
tatTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATc > 2:462332/1‑100 (MQ=18)
tatTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATc > 2:499514/1‑100 (MQ=17)
atTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCg > 1:663804/1‑100 (MQ=33)
ttGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaa < 1:831443/100‑1 (MQ=18)
tGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaa > 1:639697/1‑100 (MQ=18)
tGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaa > 2:728973/1‑100 (MQ=18)
ctctTTAAAAATCGGGAACAAGCTGAAAGTTGACGCACTAAACAACGAGAGTTGTTCGTGAGTCTCTCAAAATTTAGCAACACGATGATGAATCgaaaga > 2:1049240/1‑100 (MQ=11)
tctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaagaa > 2:1244654/1‑100 (MQ=18)
tctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaagaa > 2:79087/1‑100 (MQ=17)
ctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaagaaa < 1:267313/100‑1 (MQ=17)
tttAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAAc < 1:915920/100‑1 (MQ=18)
ttAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACa > 1:354727/1‑100 (MQ=18)
aaaaTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCt > 1:995769/1‑100 (MQ=18)
aTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCg > 1:114546/1‑100 (MQ=17)
cTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCggg > 2:602772/1‑100 (MQ=18)
gATCAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTtg < 1:426083/100‑1 (MQ=33)
tCAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTtgtg > 1:557851/1‑100 (MQ=33)
|
CGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTG > NC_000913/4168443‑4168639
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
