Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F212 I0 R1	246	122.0	6097616	93.1%	5676880	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,178,296	A→T	100%	intergenic (+8/‑151)	nusG → / → rplK	transcription termination factor/50S ribosomal subunit protein L11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,178,296	0	A	T	98.9%	443.7 / ‑4.0	141	intergenic (+8/‑151)	nusG/rplK	transcription termination factor/50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand): ref base A (1/2); new base T (81/57); total (82/59)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATT  >  NC_000913/4178199‑4178390

 aCGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCa                                                                                             <  1:2338998/100‑1 (MQ=255)

   gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCaaa                                                                                           <  1:2947588/100‑1 (MQ=255)

        aTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgc                                                                                      <  1:767540/100‑1 (MQ=255)

         tctcGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGTCCTCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcg                                                                                     <  1:122575/100‑1 (MQ=255)

           tcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcggc                                                                                   <  1:1986282/100‑1 (MQ=255)

            cGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcggcg                                                                                  >  2:645961/1‑100 (MQ=255)

               cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGAtt                                                                               <  1:2334888/100‑1 (MQ=255)

               cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGAtt                                                                               >  1:2509717/1‑100 (MQ=255)

               cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGAtt                                                                               >  1:14435/1‑100 (MQ=255)

               cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAtt                                                                               <  1:1865426/100‑1 (MQ=255)

                tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGAttt                                                                              >  2:2062209/1‑100 (MQ=255)

                tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGAttt                                                                              >  1:453214/1‑100 (MQ=255)

                 gtaaGTGTTTTGTTTTATGTTGGTTCGTGAGACCCCGGTAGTGCTGGACTTCATCAAGGTTGAAATTGGCTATCACAGCGTTAAAAAAAGCGGCGAtt                                                                               <  2:2655639/96‑1 (MQ=255)

                  aaaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTaa                                                                            >  2:1951034/1‑100 (MQ=255)

                    aGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATc                                                                          >  2:35312/1‑100 (MQ=255)

                    aGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATc                                                                          <  1:281363/100‑1 (MQ=255)

                    aGTATCTGTTTCTATCTTCGGTCGTGCGACCCTGGTAGAGCTGTACTTCAGCCAGGTTGAAAAAGCCTAATCCAGCGTTTAAAAAAGCGGCGATTTaa                                                                            <  2:2301065/98‑1 (MQ=255)

                     gtgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCg                                                                         >  1:2428935/1‑100 (MQ=255)

                        tCTGTTTGTATCTTCGGTCGTGCGACTCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTg                                                                      <  1:269277/100‑1 (MQ=255)

                        tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTg                                                                      <  2:1172038/100‑1 (MQ=255)

                        tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTg                                                                      <  1:377996/100‑1 (MQ=255)

                        tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTg                                                                      >  1:129877/1‑100 (MQ=255)

                        tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTg                                                                      >  2:941145/1‑100 (MQ=255)

                        tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTg                                                                      <  1:2182411/100‑1 (MQ=255)

                        tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCAGGACTTCAGCCAGGATGAAAAAGCATAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTg                                                                      >  2:964518/1‑100 (MQ=255)

                         cTGTTTCTATTTTCGGTCGTGCTACCTCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCTAGCGTTCAAAAAAGTGGCGATTTAATCGTTGc                                                                     <  2:1185630/100‑1 (MQ=255)

                         cTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGc                                                                     >  1:380945/1‑100 (MQ=255)

                          tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGca                                                                    >  1:383936/1‑100 (MQ=255)

                           gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTTGACTTCAGCCAAATTGAAAAAACCTAACAAAGCGTTCAAAAAAGCGGCGATTTAATAGTTGca                                                                    >  1:21634/1‑99 (MQ=255)

                            tttCTATCTTCGGTCGTGCGACCCCTGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGcaca                                                                  <  1:2480688/100‑1 (MQ=255)

                            cttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGcaca                                                                  <  2:1793336/99‑1 (MQ=255)

                             ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCCGCGTTCAAAAAAGCGGCGATTTAATCGTTg                                                                      <  2:2090096/95‑1 (MQ=255)

                             ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACaa                                                                 >  1:2317840/1‑100 (MQ=255)

                              tatATCTTCGGTCGTGCGACCCCGGTAGAGCGGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAg                                                                >  1:2045968/3‑100 (MQ=255)

                              tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGATGCACAAg                                                                >  1:2165316/1‑100 (MQ=255)

                               cTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAgg                                                               >  2:745128/1‑100 (MQ=255)

                                 aTCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                                             >  2:1467388/1‑100 (MQ=255)

                                 aTCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                                             >  1:301993/1‑100 (MQ=255)

                                   cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGTCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                                           <  1:264379/100‑1 (MQ=255)

                                   cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                                           >  2:2890593/1‑100 (MQ=255)

                                   cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                                           <  1:1401225/100‑1 (MQ=255)

                                     tCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAATAGCCTAAAAAAAAAAAAAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                                                         <  2:1386075/100‑1 (MQ=255)

                                     tCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                                                         <  2:2860626/100‑1 (MQ=255)

                                     tCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                                                         <  1:2962011/100‑1 (MQ=255)

                                        gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAtt                                                      >  2:2931271/1‑100 (MQ=255)

                                         tCGTGCTACCCCGGTATAGTTTGATTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                                                         <  1:274376/96‑1 (MQ=255)

                                          cGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTgg                                                    >  1:2851449/1‑100 (MQ=255)

                                          cGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTgg                                                    >  1:495856/1‑100 (MQ=255)

                                          cGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTgg                                                    >  1:1006942/1‑100 (MQ=255)

                                           gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGa                                                   >  2:1006987/1‑100 (MQ=255)

                                           gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGa                                                   <  1:1516444/100‑1 (MQ=255)

                                            tGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                                                  <  1:1956918/100‑1 (MQ=255)

                                             gCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                                                  >  2:1550608/1‑99 (MQ=255)

                                               gACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAc                                               <  1:2221818/100‑1 (MQ=255)

                                                    caacctggCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAttt                                          >  1:2396664/8‑100 (MQ=255)

                                                         gagCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCgcgc                                     <  2:2317814/100‑1 (MQ=255)

                                                         gagCTGGACGTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTACAAAAAAGCGGCGATTTAATCGTCGCACAAGGCGTGAGATTGGAATACAATTTCgcgc                                     >  2:2036876/1‑100 (MQ=255)

                                                          agCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCATAAAAGCGGCGATTTAATCGTTGCACAAGGCGCGAAATTGTATTCCAATCTCACGcc                                    <  1:2516189/100‑1 (MQ=25)

                                                           gCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTTATCGTTGCACAAGGCGTGAGATTGGAATACAATTTAGCGCCt                                   >  2:2775889/1‑100 (MQ=255)

                                                           gCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCt                                   >  2:661731/1‑100 (MQ=255)

                                                            cTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtt                                  >  2:469817/1‑100 (MQ=255)

                                                             tGGACTTTAGTCAGGTTGAATAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGGTGCACAAGGCGCGAAATTGTATTCCAATCTCACGCCt                                   <  1:2765167/98‑1 (MQ=16)

                                                                cttccgatctggTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGt                              <  1:2668400/90‑1 (MQ=255)

                                                                aCTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGt                              <  2:390261/100‑1 (MQ=255)

                                                                 cTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGTGGTGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtt                             <  1:939614/100‑1 (MQ=255)

                                                                 cTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtt                             >  2:2111393/1‑100 (MQ=255)

                                                                 cTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtt                             >  2:2425253/1‑100 (MQ=255)

                                                                   tCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtttt                           <  1:1953675/100‑1 (MQ=255)

                                                                    cAGCCAGGTTGAAAAAGCCTAACCAAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTAGCGCCTTTTGttttt                          >  2:1881684/1‑100 (MQ=255)

                                                                      gCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                        >  1:2921979/1‑100 (MQ=255)

                                                                       caaGGTTTCAAAAGCCTACCCCAGCGTTAAAAAAAGCGGCGATTTAATCGTTGCCCAAGGCGTGAGATTGGAATCCAATTTCGCGCCTTTTGTTTTTAta                       <  2:1349366/98‑2 (MQ=255)

                                                                         acgTTGAAAAAGCGTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATggg                     >  1:2061617/3‑100 (MQ=255)

                                                                         aGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATgg                      <  1:714542/99‑1 (MQ=255)

                                                                         aGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGACGTGAGATTGGAATACAATTTCGCACCTTTTGTTTTTAt                        >  1:1159448/1‑97 (MQ=255)

                                                                         aGGTTGAAAAAGCCTAACCCAGCGTTAAAAAAAGCGGCAATTTAATCGTTGCACAAGGCGTGAGATTGTAATACAATTTCGCGCCTTTTGTTTTTATg                       <  2:2119805/98‑1 (MQ=255)

                                                                          ggTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtagatcggaag                    >  2:2668368/1‑90 (MQ=255)

                                                                          ggTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGc                    >  1:2418443/1‑100 (MQ=255)

                                                                          ggTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGc                    >  1:1864882/1‑100 (MQ=255)

                                                                          ggTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGc                    >  2:386506/1‑100 (MQ=255)

                                                                          ggTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAATTCGCACCTTTTGTTTTTa                         >  1:2073994/1‑95 (MQ=255)

                                                                           gTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGcc                   >  2:167746/1‑100 (MQ=255)

                                                                           gTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGcc                   >  1:2065763/1‑100 (MQ=255)

                                                                             tGAAAAAGCCTAAGCCAGCGTTCAAAAAAGCGGCGATTTAATGGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCtt                 >  1:2050720/1‑100 (MQ=255)

                                                                             tGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGcc                   >  1:1715209/1‑98 (MQ=255)

                                                                             tGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCtt                 >  1:1864005/1‑100 (MQ=255)

                                                                              gAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCATAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGcc                   <  2:2415002/97‑1 (MQ=255)

                                                                              gAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                >  1:1723129/1‑100 (MQ=255)

                                                                              gAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                >  2:2271409/1‑100 (MQ=255)

                                                                              gAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                <  1:2983793/100‑1 (MQ=255)

                                                                               aaaaaGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGc               >  1:1008596/1‑100 (MQ=255)

                                                                                 aaaGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTTATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGccc             <  1:2775921/100‑1 (MQ=255)

                                                                                   aGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGt           <  2:787312/100‑1 (MQ=255)

                                                                                    gCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGACTTGACCGTa          >  1:610363/1‑100 (MQ=255)

                                                                                       tacccaaGCGTTAAAAAAAGCGGCGTTTTAATCTTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaan        <  2:1345690/93‑2 (MQ=255)

                                                                                       tAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaaa       >  2:2116653/1‑100 (MQ=255)

                                                                                       tAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaaa       >  1:2056239/1‑100 (MQ=255)

                                                                                        aaCCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaa        >  1:2644535/1‑98 (MQ=255)

                                                                                         aCCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAGTACAATTTCGCGCCTTTTTTTTTTACGGGCAAGGCCCATaaaaac     <  2:590786/100‑3 (MQ=255)

                                                                                            cAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtt  <  2:3016661/100‑1 (MQ=255)

                                                                                             aGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTACGGGCAAGGCCCATaaaaaca    <  2:813286/97‑4 (MQ=37)

TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATT  >  NC_000913/4178199‑4178390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A