Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F256 I1 R1 186 136.5 6958264 92.1% 6408561 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,178,296 A→T intergenic (+8/‑151) nusG → / → rplK transcription termination factor/50S ribosomal subunit protein L11

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,178,2960AT97.7% 426.7 / ‑2.0 128intergenic (+8/‑151)nusG/rplKtranscription termination factor/50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (63/62);  total (64/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 128 total aligned reads displayed.

ACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTT  >  NC_000913/4178200‑4178391
                                                                                                |                                                                                               
aCGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCa                                                                                              <  1:2161224/100‑1 (MQ=255)
 cGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCaa                                                                                             <  2:744616/100‑1 (MQ=255)
  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCaaa                                                                                            >  2:210839/1‑100 (MQ=255)
   agaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCaaaa                                                                                           >  1:2553596/1‑100 (MQ=255)
         ctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcgg                                                                                     <  1:3377183/100‑1 (MQ=255)
         ctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcgg                                                                                     >  1:738273/1‑100 (MQ=255)
         ctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcgg                                                                                     >  2:343695/1‑100 (MQ=255)
         ctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcg                                                                                      <  2:958634/99‑1 (MQ=255)
         ctcGTCTGAAAGTGTCTGTTTCTATCTTCGGACGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAACAGCCTAACCCAGAGTTCAAAAAAgcgg                                                                                     >  2:538748/1‑100 (MQ=255)
          tcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcggc                                                                                    <  2:3014003/100‑1 (MQ=255)
           cGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcggcg                                                                                   >  1:2824291/1‑100 (MQ=255)
            gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGa                                                                                  >  2:3270793/1‑100 (MQ=255)
            gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGa                                                                                  >  2:271829/1‑100 (MQ=255)
             tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGAt                                                                                 <  2:2123342/100‑1 (MQ=255)
             tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGAt                                                                                 >  2:189707/1‑100 (MQ=255)
               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGAttt                                                                               <  1:173245/100‑1 (MQ=255)
               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGAttt                                                                               <  2:1286914/100‑1 (MQ=255)
                gAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGAACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTa                                                                              >  1:1927892/1‑100 (MQ=255)
                  aaGTGTTTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAAt                                                                            <  1:1154696/100‑1 (MQ=255)
                    gtgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCg                                                                          <  2:1523196/100‑1 (MQ=255)
                      gtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGtt                                                                        >  2:1865448/1‑100 (MQ=255)
                      gtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGtt                                                                        >  1:2592944/1‑100 (MQ=255)
                      gtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGtt                                                                        <  2:1599929/100‑1 (MQ=255)
                       tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTg                                                                       <  2:2832757/100‑1 (MQ=255)
                       tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTg                                                                       <  2:738284/100‑1 (MQ=255)
                       tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTg                                                                       <  1:919894/100‑1 (MQ=255)
                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGcac                                                                    >  2:1925982/1‑100 (MQ=255)
                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGcac                                                                    >  2:834948/1‑100 (MQ=255)
                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTAAAAAAAAAAAAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTg                                                                       <  1:1346673/97‑1 (MQ=255)
                          gTTTCGATCTTCGGTCGTGCGACCCCCGTAGAGCTGGAGTTCAGCCAGGTTGAAATAGCCTAAAAAAAAAAAAAAAAAAGCGGCGATTTAATCGTTGc                                                                      <  1:2248294/98‑1 (MQ=255)
                            ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACaa                                                                  >  2:1177337/1‑100 (MQ=255)
                              cTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAgg                                                                >  2:1809403/1‑100 (MQ=255)
                               tATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGc                                                               >  1:3417209/1‑100 (MQ=255)
                                  cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                                            >  2:2410829/1‑100 (MQ=255)
                                  cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                                            >  1:985735/1‑100 (MQ=255)
                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTga                                                           <  2:1862445/100‑1 (MQ=255)
                                     cGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgaga                                                         >  1:842647/1‑100 (MQ=255)
                                      ggTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                                                        >  2:973860/1‑100 (MQ=255)
                                         cGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTgg                                                     <  1:3076972/100‑1 (MQ=255)
                                         cGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTgg                                                     <  2:2597927/100‑1 (MQ=255)
                                          gTGCGACCCCGGTAGAGCTGGACTTCATCCATGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGa                                                    <  2:3449146/100‑1 (MQ=255)
                                          gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGa                                                    <  1:385964/100‑1 (MQ=255)
                                          gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGa                                                    <  1:1710726/100‑1 (MQ=255)
                                          gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGa                                                    >  2:3137227/1‑100 (MQ=255)
                                           tGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                                                   <  1:2966220/100‑1 (MQ=255)
                                           tGCGACCCCGGTAAAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAAAAAAAAAAAAAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                                                   <  1:3345529/100‑1 (MQ=255)
                                               aCCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGCGATTGGAATACa                                               >  2:1515147/1‑100 (MQ=255)
                                                ccccGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACaa                                              >  2:762503/1‑100 (MQ=255)
                                                     gTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcg                                         <  1:907635/100‑1 (MQ=255)
                                                     gTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcg                                         <  2:446699/100‑1 (MQ=255)
                                                      tAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcgc                                        <  1:1920429/100‑1 (MQ=255)
                                                        gagCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCgcgc                                      <  1:2628936/100‑1 (MQ=255)
                                                         agCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGcc                                     >  1:3474703/1‑100 (MQ=255)
                                                            tGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTTAAAAAAGCGGCGATTTAATAGTTGCACAAGGCGTGAGATTGGAATACAATTTTGAGTCttt                                  >  1:2950703/1‑100 (MQ=255)
                                                            tGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt                                  >  2:813760/1‑100 (MQ=255)
                                                            tGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt                                  >  1:2608903/1‑100 (MQ=255)
                                                               aCTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGCGAAATTGTATTCCAATCTCACGCCTtgtgc                               <  2:791052/100‑2 (MQ=18)
                                                                   cAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttttt                           >  1:2124585/1‑100 (MQ=255)
                                                                   cAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTAAGATGGGAATACAATTTCGCTCCTGTTGtttt                            >  1:3251553/1‑99 (MQ=255)
                                                                   cAGCCAGGCTCAAAAAGCCGAACCCAGAGTTCCAAAAAGCAGAGATTCAATCGTTGCACAAGGCGTGAATTGGGAAAACAATTTTGCTACttttat                               >  1:2485549/1‑94 (MQ=255)
                                                                    aGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTa                          <  2:2076992/100‑1 (MQ=255)
                                                                     gCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                         <  2:3355662/100‑1 (MQ=255)
                                                                     gCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                         >  2:950496/1‑100 (MQ=255)
                                                                     gCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                         <  2:274613/100‑1 (MQ=255)
                                                                     gCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                         >  1:2114533/1‑100 (MQ=255)
                                                                       cAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATgg                       <  2:2419321/100‑1 (MQ=255)
                                                                         ggTTGAAAAAGTCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGc                     >  1:254942/1‑100 (MQ=255)
                                                                         ggTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGc                     >  1:2989933/1‑100 (MQ=255)
                                                                          gTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGcc                    >  1:1768951/1‑100 (MQ=255)
                                                                          gTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGcc                    <  2:1341443/100‑1 (MQ=255)
                                                                           ttGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCt                   >  2:739204/1‑100 (MQ=255)
                                                                           tagAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAAGCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCt                   >  2:2344897/3‑100 (MQ=255)
                                                                            tGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCtt                  >  2:2487621/1‑100 (MQ=255)
                                                                            tGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCtt                  >  2:2402804/1‑100 (MQ=255)
                                                                             gAAATAGCCTAACCCAGCGTTCAAAAAAGCGGCAATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                 <  1:1692892/100‑1 (MQ=255)
                                                                             gAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                 <  1:754038/100‑1 (MQ=255)
                                                                             gAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                 >  2:1568934/1‑100 (MQ=255)
                                                                             gAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                 <  1:662501/100‑1 (MQ=255)
                                                                              aaaaaGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGc                >  1:3203569/1‑100 (MQ=255)
                                                                              aaaaaGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGc                >  2:376338/1‑100 (MQ=255)
                                                                                 aaGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg             <  1:2392608/100‑1 (MQ=255)
                                                                                 aaGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg             <  2:2824335/100‑1 (MQ=255)
                                                                                 aaGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg             <  2:3132774/100‑1 (MQ=255)
                                                                                 aaGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg             <  2:1066850/100‑1 (MQ=255)
                                                                                  aGCCTAACCCTGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGt            <  1:2280801/100‑1 (MQ=255)
                                                                                  aGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGt            <  1:1577414/100‑1 (MQ=255)
                                                                                   gCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACACTTTCGCGCCTTTTGTTTTTATGGGACTTGCCCGTa           >  1:1660250/1‑100 (MQ=255)
                                                                                   gCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTa           <  1:2633523/100‑1 (MQ=255)
                                                                                   gCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTa           <  2:1121445/100‑1 (MQ=255)
                                                                                    ccTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaa          <  1:2850716/100‑1 (MQ=255)
                                                                                    ccTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaa          <  2:2592979/100‑1 (MQ=255)
                                                                                     cTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaa         <  2:370599/100‑1 (MQ=255)
                                                                                     cTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaa         >  2:1333901/1‑100 (MQ=255)
                                                                                      tAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCTTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaaa        <  1:131196/100‑1 (MQ=255)
                                                                                         cccAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGa     >  1:1750672/1‑100 (MQ=255)
                                                                                         cccAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGa     >  1:1934576/1‑100 (MQ=255)
                                                                                          ccAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAt    <  2:194200/100‑1 (MQ=255)
                                                                                          ccAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAt    <  2:1092379/100‑1 (MQ=255)
                                                                                           cAGCGTTCAAAAAAGCGGCGATTTAGTGGTGTGGCAAGTCGTGAGATTGGAGTAGAGTTTCGCGCCTTTTGTGTGGATGGTCCTTGCCCGTAAAACGAtt   >  2:3256934/1‑100 (MQ=255)
                                                                                            aGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAttt  <  1:1032852/100‑1 (MQ=255)
                                                                                                |                                                                                               
ACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTT  >  NC_000913/4178200‑4178391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A