Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,616,491 |
T→C |
D192G (GAT→GGT) |
yjjI ← |
DUF3029 family protein, putative glycine radical enzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,616,491 | 0 | T | C | 89.1%
| 154.1
/ 9.0
| 55 | D192G (GAT→GGT) | yjjI | DUF3029 family protein, putative glycine radical enzyme |
| Reads supporting (aligned to +/- strand): ref base T (5/1); new base C (28/21); total (33/22) |
| Fisher's exact test for biased strand distribution p-value = 3.84e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 |
ATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGG > NC_000913/4616397‑4616587
|
aTCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAg < 2:649102/100‑1 (MQ=255)
tCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGa < 1:198460/100‑1 (MQ=255)
aTGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATa > 1:670664/1‑100 (MQ=255)
gCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATaaa > 2:457783/1‑100 (MQ=255)
cACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAg < 1:1129251/100‑1 (MQ=255)
cACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAg < 1:1129315/100‑1 (MQ=255)
aCCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAgg < 2:725141/100‑1 (MQ=255)
aCCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAgg < 2:725134/100‑1 (MQ=255)
cGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGt > 2:402777/1‑99 (MQ=255)
ggACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCa > 1:924121/1‑100 (MQ=255)
ggACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCa < 1:689235/100‑1 (MQ=255)
ccGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGt < 1:651776/100‑1 (MQ=255)
ggCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGt < 1:1276264/100‑1 (MQ=255)
gCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTg > 2:104781/1‑100 (MQ=255)
tgCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCt > 1:1298532/1‑100 (MQ=255)
tgCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCt > 2:705324/1‑100 (MQ=255)
tgCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCt > 1:1117576/1‑100 (MQ=255)
gTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCtt > 1:1290754/1‑100 (MQ=255)
gTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCtt > 2:656977/1‑100 (MQ=255)
tttGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTc < 2:939124/100‑1 (MQ=255)
tttGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTc > 2:749659/1‑100 (MQ=255)
ttGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTcn < 1:540407/100‑2 (MQ=255)
ttGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCa > 2:1275523/1‑100 (MQ=255)
ttGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCa > 1:62131/1‑100 (MQ=255)
cTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAAct > 2:1152174/1‑100 (MQ=255)
tACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAActc > 1:300265/1‑100 (MQ=255)
aCATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAActct > 2:1047222/1‑100 (MQ=255)
aTTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACtctgc > 2:831689/1‑100 (MQ=255)
aTTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACtctgc > 1:106011/1‑100 (MQ=255)
ttCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACtctgca > 2:413993/1‑100 (MQ=255)
ttCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACtctgca < 1:1033771/100‑1 (MQ=255)
gTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGt > 1:585203/1‑100 (MQ=255)
cttcGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAg > 2:263805/1‑100 (MQ=255)
gCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATcg > 2:3659/1‑100 (MQ=255)
cTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATcgcgcg < 2:1003791/100‑1 (MQ=255)
tCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCggg > 2:1150936/1‑100 (MQ=255)
tCCAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCggg < 2:260905/100‑1 (MQ=255)
ccAGCAGCAGGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGt > 1:754401/1‑100 (MQ=255)
gcagcagGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAAt < 1:522472/100‑1 (MQ=255)
gcagGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATggg > 2:644244/1‑100 (MQ=255)
cagGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGc < 2:168014/100‑1 (MQ=255)
cagGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGc < 2:167652/100‑1 (MQ=255)
agGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCg > 1:577082/1‑100 (MQ=255)
agGTCATCAGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCg < 1:635465/100‑1 (MQ=255)
tcatcaGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAAt < 1:543821/100‑1 (MQ=255)
catcaGGGGTGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATc > 2:91597/1‑100 (MQ=255)
gggTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACgg > 1:238528/1‑100 (MQ=255)
tGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGcc < 2:55885/100‑1 (MQ=255)
tGATTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGcc < 1:1150947/100‑1 (MQ=255)
aTTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGa > 1:522895/1‑100 (MQ=255)
aTTTCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGa > 1:1219804/1‑100 (MQ=255)
tttCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGat > 1:126365/1‑100 (MQ=255)
tCAGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGatat > 2:245722/1‑100 (MQ=255)
aGGACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTg < 1:749664/100‑1 (MQ=255)
ggACCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTgg > 1:1232328/1‑100 (MQ=255)
|
ATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGGGCGAATCAGACGGGCCGATATTGG > NC_000913/4616397‑4616587
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
