Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F204 I1 R1 117 93.2 4682243 93.4% 4373214 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,540,351 (A)6→5 coding (189/228 nt) feoA → ferrous iron transporter, protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,540,3460A.94.9% 329.0 / 12.7 79coding (184/228 nt)feoAferrous iron transporter, protein A
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base . (35/39);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCC  >  NC_000913/3540247‑3540441
                                                                                                   |                                                                                               
ctTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCa                                                                                                 <  2:509880/100‑1 (MQ=255)
ctTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCa                                                                                                 <  2:528699/100‑1 (MQ=255)
       tGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCaaaaagat                                                                                          >  2:316198/1‑97 (MQ=255)
        gTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCaaaaagatc                                                                                         <  1:964916/100‑5 (MQ=255)
         ttACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCt                                                                                       <  1:55260/100‑1 (MQ=255)
           aCCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTgg                                                                                     <  1:2153743/100‑1 (MQ=255)
            ccTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGc                                                                                    >  1:846248/1‑100 (MQ=255)
             cTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGcc                                                                                   <  2:496687/100‑1 (MQ=255)
               ggCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCtt                                                                                 >  1:1228293/1‑100 (MQ=255)
               ggCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCtt                                                                                 >  2:541163/1‑100 (MQ=255)
                 ctcctcTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGCGCCTGGTATTACGC‑AAAAAGATCTGGCCttat                                                                               >  1:8111/1‑100 (MQ=255)
                 ctcctcTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCttat                                                                               >  2:1335534/1‑100 (MQ=255)
                   cctcTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCttatta                                                                             >  2:581229/1‑100 (MQ=255)
                    ctctTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCt                                                                                  >  1:423686/1‑94 (MQ=255)
                    ctctTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAg                                                                            >  1:2035118/1‑100 (MQ=255)
                     tctTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGa                                                                           <  2:2089688/100‑1 (MQ=255)
                     tctTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCT‑AAAATAAAAAATAAAGATCTGGCCTTATTAGa                                                                           <  2:1428782/100‑1 (MQ=255)
                       ttttAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAACCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAg                                                                         <  1:742034/100‑1 (MQ=255)
                        cgatctgtgGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGt                                                                        <  2:639436/95‑1 (MQ=255)
                          tAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTgg                                                                      <  2:328236/100‑1 (MQ=255)
                          tAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTgg                                                                      <  2:869801/100‑1 (MQ=255)
                           aaTGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTgg                                                                      >  2:845243/1‑100 (MQ=255)
                           aaTGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGa                                                                     <  1:581230/100‑1 (MQ=255)
                             tgtgGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAg                                                                   <  1:1761460/100‑1 (MQ=255)
                              gtggtgCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGtagatcg                                                                  >  1:639437/1‑94 (MQ=255)
                              gtggtgCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGc                                                                  >  1:450392/1‑100 (MQ=255)
                               tggtgCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCg                                                                 <  1:1247959/100‑1 (MQ=255)
                                ggtgCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCgg                                                                >  2:1256650/1‑100 (MQ=255)
                                   gcgcGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGttt                                                             >  1:1282348/1‑100 (MQ=255)
                                    cgcgTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTc                                                            >  1:1232335/1‑100 (MQ=255)
                                     gcgTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTcc                                                           <  2:2081209/100‑1 (MQ=255)
                                     gcgTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTcc                                                           >  2:1513137/1‑100 (MQ=255)
                                     gcgTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTcc                                                           <  1:148544/100‑1 (MQ=255)
                                     gatctgCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTcc                                                           <  2:766360/95‑1 (MQ=255)
                                       gTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTg                                                         <  2:1833500/100‑1 (MQ=255)
                                        tCGCTCCGCTCGGCGACGCCATTAATATCGAAAGCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTCTTAGGAGTGGAAGCGGTTTCCTg                                                         <  2:464252/100‑1 (MQ=255)
                                        tCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGt                                                        >  2:2335735/1‑100 (MQ=255)
                                        tCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACG‑TAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGt                                                        <  2:1279700/100‑1 (MQ=255)
                                         cGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGtt                                                       <  2:2271579/100‑1 (MQ=255)
                                          gCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTccagatc                                                      >  1:766361/1‑95 (MQ=255)
                                            tCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAAt                                                    >  1:196094/1‑100 (MQ=255)
                                            tCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAAt                                                    <  2:486994/100‑1 (MQ=255)
                                             ccACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATa                                                   >  2:1876254/1‑100 (MQ=255)
                                              cACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATAc                                                  >  1:2025895/1‑100 (MQ=255)
                                                  cGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTg                                              <  2:1483825/100‑1 (MQ=255)
                                                       aCCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATaac                                         <  1:1618039/100‑1 (MQ=255)
                                                          ccATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATaacaac                                      >  1:1567365/1‑100 (MQ=255)
                                                           cATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAacaaca                                     <  1:1006967/100‑1 (MQ=255)
                                                           cATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAacaaca                                     >  1:579114/1‑100 (MQ=255)
                                                            aTTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAAcaacaa                                    <  2:1228984/100‑1 (MQ=255)
                                                            aTTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAAcaacaa                                    <  2:8111/100‑1 (MQ=255)
                                                              tCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATg                                  <  2:2146478/100‑1 (MQ=255)
                                                                 tatCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGaaa                               >  2:1071463/1‑100 (MQ=255)
                                                                 tatCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGaaa                               <  2:423684/100‑1 (MQ=255)
                                                                  atCGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGaaaa                              >  1:291229/1‑100 (MQ=255)
                                                                    cGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGaaaaaa                            >  2:1127986/1‑100 (MQ=255)
                                                                    cGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGaaaaaa                            >  2:848071/1‑100 (MQ=255)
                                                                    cGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGaaaaaa                            >  2:2279347/1‑100 (MQ=255)
                                                                    cGAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGaaaaaa                            >  2:952560/1‑100 (MQ=255)
                                                                     gAAACCCGTCGTGTTAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAAt                           <  1:573582/100‑1 (MQ=255)
                                                                     gAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAAt                           >  2:490318/1‑100 (MQ=255)
                                                                     gAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAAt                           >  2:2282228/1‑100 (MQ=255)
                                                                     gAAACCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAAt                           >  1:1330551/1‑100 (MQ=255)
                                                                      aaaCCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAAtt                          <  1:490320/100‑1 (MQ=255)
                                                                       aaCCCGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTa                         >  1:1222241/1‑100 (MQ=255)
                                                                         cccGTCGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAAc                       <  1:58390/100‑1 (MQ=255)
                                                                          ccGTTGTGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAAcc                      <  2:751066/100‑1 (MQ=255)
                                                                             tcgtGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCAtt                   <  2:450390/100‑1 (MQ=255)
                                                                              cgtGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCAtt                   <  1:637245/100‑1 (MQ=255)
                                                                              cgtGTGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTg                  >  1:170957/1‑100 (MQ=255)
                                                                              cgtGTGAGCCTAATAATAAGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTg                  <  2:1282345/100‑1 (MQ=255)
                                                                               gtgtGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTgg                 <  1:1707702/100‑1 (MQ=255)
                                                                               gtgtGAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAATGTAAGCGGTTTTCTGTTTATACGGTGATAAAAACAATGAAGAAATTAACCATTg                  <  2:1449660/99‑1 (MQ=255)
                                                                                  tgAGCCTGGTATTACGC‑AAAATGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTCAATACGGTGATAAAAAAAAAAAAAAAATTAAcc                      <  2:1405874/92‑1 (MQ=255)
                                                                                  tgAGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCtt              <  2:1567361/100‑1 (MQ=255)
                                                                                    aGCCTGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTaa            >  1:248698/1‑100 (MQ=255)
                                                                                        tGGTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTgg        <  2:1228290/100‑1 (MQ=255)
                                                                                         ggTATTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGt       >  1:1819757/1‑100 (MQ=255)
                                                                                            aTTACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAAt    <  1:1256653/100‑1 (MQ=255)
                                                                                             ttACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATc   <  2:119493/100‑1 (MQ=255)
                                                                                              tACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATcc  >  2:641097/1‑100 (MQ=255)
                                                                                              tACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATcc  <  2:1244947/100‑1 (MQ=255)
                                                                                              tACGC‑AAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATcc  <  2:1554839/100‑1 (MQ=255)
                                                                                                   |                                                                                               
CTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTAATTGGTAATCC  >  NC_000913/3540247‑3540441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A