Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F204 I1 R1 117 93.2 4682243 93.4% 4373214 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,704,719 G→T P15T (CCG→ACG)  dppC ← dipeptide/heme ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,704,7190GT98.8% 286.7 / ‑3.5 81P15T (CCG→ACG) dppCdipeptide/heme ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (36/44);  total (37/44)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

CGCGATGAACAGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGGCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAG  >  NC_000913/3704624‑3704815
                                                                                               |                                                                                                
cgcgATGAACAGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCAcc                                                                                              <  2:9561/100‑1 (MQ=255)
cgcgATGAACAGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCAcc                                                                                              <  2:700604/100‑1 (MQ=255)
 gcgATGAACAGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCg                                                                                             >  1:406224/1‑100 (MQ=255)
  cgATGAACAGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCgg                                                                                            <  2:753114/100‑1 (MQ=255)
  cgATGAACAGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCgg                                                                                            >  2:2040157/1‑100 (MQ=255)
       aaCAGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTgcgc                                                                                       <  2:1410610/100‑1 (MQ=255)
        aCAGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCt                                                                                      >  1:984351/1‑100 (MQ=255)
        aCAGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCt                                                                                      <  1:356977/100‑1 (MQ=255)
         cAGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTa                                                                                     >  2:1121774/1‑100 (MQ=255)
          aGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTaa                                                                                    >  2:1538157/1‑100 (MQ=255)
          aGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTGGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTaa                                                                                    <  1:2184406/100‑1 (MQ=255)
           gCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAAt                                                                                   >  1:1323645/1‑100 (MQ=255)
            cACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATc                                                                                  <  2:1227766/100‑1 (MQ=255)
            cACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATc                                                                                  <  2:1681260/100‑1 (MQ=255)
             aCGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATc                                                                                  <  1:2244196/99‑1 (MQ=255)
              cGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCAc                                                                                >  2:1480113/1‑100 (MQ=255)
              cGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCAc                                                                                >  2:306070/1‑100 (MQ=255)
                   acgacgTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTAt                                                                           >  2:538043/1‑100 (MQ=255)
                    cgacgTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTAtt                                                                          >  2:1063777/1‑100 (MQ=255)
                      acgTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTAtttt                                                                        >  2:1551307/1‑100 (MQ=255)
                      acgTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTAtttt                                                                        <  2:2067211/100‑1 (MQ=255)
                       cgTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTc                                                                       <  1:1378928/100‑1 (MQ=255)
                       cgTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTc                                                                       <  1:1551311/100‑1 (MQ=255)
                       cgTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTc                                                                       <  2:2175636/100‑1 (MQ=255)
                         tAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAg                                                                     <  2:2237290/100‑1 (MQ=255)
                          aaaCCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTTACCGGTGCGCTAATCACTTTATTTTCAGt                                                                    <  1:1432835/100‑1 (MQ=255)
                           aaCCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTa                                                                   >  1:1482287/1‑100 (MQ=255)
                            aCCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTaa                                                                  >  1:849670/1‑100 (MQ=255)
                              cAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAAcc                                                                <  1:427325/100‑1 (MQ=255)
                              cAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAAcc                                                                <  2:406222/100‑1 (MQ=255)
                                gCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCtg                                                              <  2:773001/100‑1 (MQ=255)
                                  ccGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCtgtg                                                            >  2:411111/1‑100 (MQ=255)
                                   cgacgaCCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGa                                                           <  1:950688/100‑1 (MQ=255)
                                    gacgacCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGAc                                                          <  1:1121776/100‑1 (MQ=255)
                                       gacCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACatg                                                       <  2:473979/100‑1 (MQ=255)
                                        acCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACatga                                                      >  1:124902/1‑100 (MQ=255)
                                         ccGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACatgat                                                     <  1:184776/100‑1 (MQ=255)
                                          cgcgCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACatgatg                                                    <  1:2137123/100‑1 (MQ=255)
                                           gcgcCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGt                                                   >  2:892053/1‑100 (MQ=255)
                                              ccTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTccc                                                <  2:2061651/100‑1 (MQ=255)
                                                   gttgCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTAc                                           <  1:538045/100‑1 (MQ=255)
                                                    ttgCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTAct                                          >  1:1657537/1‑100 (MQ=255)
                                                    ttgCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTAct                                          <  1:1538161/100‑1 (MQ=255)
                                                         tttAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTa                                     <  2:1323642/100‑1 (MQ=255)
                                                         tttAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTa                                     >  1:553595/1‑100 (MQ=255)
                                                          ttAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTAt                                    <  1:1063779/100‑1 (MQ=255)
                                                          ttAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTAt                                    <  1:1166849/100‑1 (MQ=255)
                                                          ttAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTAt                                    >  1:797715/1‑100 (MQ=255)
                                                           tAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGGCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATg                                   >  1:208055/1‑100 (MQ=255)
                                                            aaaaTAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGa                                  <  2:1491271/100‑1 (MQ=255)
                                                             aaaTAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGAc                                 <  1:963240/100‑1 (MQ=255)
                                                             aaaTAGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGAc                                 >  1:1273595/1‑100 (MQ=255)
                                                                 aGTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAAt                             <  2:1657532/100‑1 (MQ=255)
                                                                  gTGCCAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATa                            <  1:1480117/100‑1 (MQ=255)
                                                                     ccAGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATAcgc                         >  2:214068/1‑100 (MQ=255)
                                                                       aGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCgg                       >  1:812066/1‑100 (MQ=255)
                                                                       aGAACTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCgg                       <  1:1251768/100‑1 (MQ=255)
                                                                           cTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTc                   >  2:1876236/1‑100 (MQ=255)
                                                                           cTCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTc                   >  1:1980974/1‑100 (MQ=255)
                                                                            tCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTca                  <  2:1266516/100‑1 (MQ=255)
                                                                            tCCTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTca                  <  2:2106517/100‑1 (MQ=255)
                                                                             ccTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTcac                 >  2:984701/1‑100 (MQ=255)
                                                                             ccTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTcac                 >  1:2327720/1‑100 (MQ=255)
                                                                             ccTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTcac                 >  1:374060/1‑100 (MQ=255)
                                                                              cTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTcacc                <  2:984349/100‑1 (MQ=255)
                                                                              cTGTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTcacc                >  1:508330/1‑100 (MQ=255)
                                                                                gTAACGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTcaccac              >  2:1440552/1‑100 (MQ=255)
                                                                                   tctgggTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGcc           <  2:260662/97‑1 (MQ=255)
                                                                                   aCGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGcc           <  2:203964/100‑1 (MQ=255)
                                                                                    cGGGGTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCg          >  2:2296933/1‑100 (MQ=255)
                                                                                     ggggTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGt         >  2:2179610/1‑100 (MQ=255)
                                                                                      gggTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGccaga        >  1:260664/1‑97 (MQ=255)
                                                                                       ggTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTAc       <  1:214070/100‑1 (MQ=255)
                                                                                       gatCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTAc       <  2:1432312/98‑1 (MQ=255)
                                                                                        gTCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTAca      >  1:1062899/1‑100 (MQ=255)
                                                                                         tCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTAcag     <  1:2094325/100‑1 (MQ=255)
                                                                                         tCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTAcag     <  1:2094342/100‑1 (MQ=255)
                                                                                         tCATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCCGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTAcag     <  1:306072/100‑1 (MQ=255)
                                                                                          cATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTAcagc    >  2:725917/1‑100 (MQ=255)
                                                                                          cATCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTAcagc    >  2:2204214/1‑100 (MQ=255)
                                                                                            tCGTCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTAcagcag  >  1:2337610/1‑100 (MQ=255)
                                                                                               |                                                                                                
CGCGATGAACAGCACGATGACGACGTAAACCAGCCCGACGACCGCGCCTTTGTTGCGTTTAAAATAGTGCCAGAACTCCTGTAACGGGGTCATCGGCACCGGTGCGCTAATCACTTTATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAG  >  NC_000913/3704624‑3704815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A