Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,998,071	G→T	G30V (GGC→GTC)	uvrD →	DNA‑dependent ATPase I and helicase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,998,071	0	G	T	100.0%	155.0 / NA	43	G30V (GGC→GTC)	uvrD	DNA‑dependent ATPase I and helicase II
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (20/23); total (20/23)

TGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAA  >  NC_000913/3997930‑3998210

tGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGgcggtcgcggg                                                                                                                                       >  1:574513/1‑148 (MQ=255)

tGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCa                                                                                                                                     <  2:76696/150‑1 (MQ=255)

 ggCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAg                                                                                                                                    <  1:635588/150‑1 (MQ=255)

           gTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGAcg                                                                                                                          >  2:336385/1‑150 (MQ=255)

             tGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGGGGTAAGAcgcg                                                                                                                        >  1:465765/1‑150 (MQ=255)

                   tctcCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACt                                                                                                                  >  1:479709/1‑150 (MQ=255)

                   tctcCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTg                                                                                                                 >  2:573625/1‑151 (MQ=255)

                   tctcCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCAGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACt                                                                                                                  <  1:71155/150‑1 (MQ=255)

                      ccGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTg                                                                                                              <  1:646366/151‑1 (MQ=255)

                       cGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGc                                                                                                             >  2:719853/1‑151 (MQ=255)

                        gCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGc                                                                                                             >  2:599573/1‑150 (MQ=255)

                           cAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCg                                                                                                         >  1:376651/1‑151 (MQ=255)

                           cAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCg                                                                                                         >  2:400687/1‑151 (MQ=255)

                               cTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATc                                                                                                     >  1:591997/1‑151 (MQ=255)

                                 aTTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCg                                                                                                    <  2:55711/150‑1 (MQ=255)

                                  tttttACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGcc                                                                                                  >  1:150162/1‑151 (MQ=255)

                                       aCGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGtt                                                                                             >  2:476954/1‑151 (MQ=255)

                                             cggTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAg                                                                                       <  2:451556/151‑1 (MQ=255)

                                                 gCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGt                                                                                    <  1:624039/150‑1 (MQ=255)

                                                     aTGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGaaa                                                                               <  1:294394/151‑1 (MQ=255)

                                                     aTGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGaa                                                                                >  1:767040/1‑150 (MQ=255)

                                                             ttCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTc                                                                        >  1:620674/1‑150 (MQ=255)

                                                                   ccTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATAc                                                                 <  2:600777/151‑1 (MQ=255)

                                                                     tGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTc                                                               >  2:257921/1‑151 (MQ=255)

                                                                            cAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATg                                                        >  1:573302/1‑151 (MQ=255)

                                                                                  tAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGt                                                   <  1:188476/150‑1 (MQ=255)

                                                                                   aaTGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGa                                                 <  2:499431/151‑1 (MQ=255)

                                                                                       aCAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGtt                                             <  2:62952/151‑1 (MQ=255)

                                                                                       aCAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGGGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCggggggg                                               >  1:751085/1‑144 (MQ=255)

                                                                                        cAATCAGCGCGCAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGGGGGAAGACGCGCGTACTGGTGAATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATTCTCGATTATGGCg                                                     >  1:601611/1‑142 (MQ=255)

                                                                                         aaaCAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTa                                           <  1:81122/151‑1 (MQ=255)

                                                                                          aaCAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGGAAACTGCTCGCCATACTCGATTATGGCgggcgggctt                                            >  1:40174/1‑141 (MQ=255)

                                                                                                gcgAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACcaacaa                                    <  2:748966/151‑1 (MQ=255)

                                                                                                gcgAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACcaacaa                                    <  2:364642/151‑1 (MQ=255)

                                                                                                gcgAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGGAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCggggggggt                                             >  1:619197/1‑135 (MQ=255)

                                                                                                  gAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGGGGCGGTTTCcaaca                                     >  1:401937/1‑148 (MQ=255)

                                                                                                    aGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgcg                                <  2:214444/151‑1 (MQ=255)

                                                                                                      cGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgcggc                              <  1:561160/151‑1 (MQ=255)

                                                                                                      cGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgcggc                              <  2:74337/151‑1 (MQ=255)

                                                                                                       ggTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgcggcg                             <  1:156107/151‑1 (MQ=255)

                                                                                                          ggCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAgcggcggcg                          <  2:574568/151‑1 (MQ=255)

                                                                                                                       agcaACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATc             <  1:476903/151‑1 (MQ=255)

                                                                                                                        gcaACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCg            <  2:381568/151‑1 (MQ=255)

                                                                                                                                  ggTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCaa  <  2:685021/151‑1 (MQ=255)

TGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAA  >  NC_000913/3997930‑3998210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A