Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,998,071	G→T	G30V (GGC→GTC)	uvrD →	DNA‑dependent ATPase I and helicase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,998,071	0	G	T	100.0%	126.2 / NA	38	G30V (GGC→GTC)	uvrD	DNA‑dependent ATPase I and helicase II
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (17/21); total (17/21)

AGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTG‑ACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATC‑GGGCAACTGATGGG  >  NC_000913/3997936‑3998218

aGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGGAGTGGTa                                                                                                                                         >  2:647169/1‑150 (MQ=255)

    cGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGAcg                                                                                                                                    <  1:857702/151‑1 (MQ=255)

        gcgcTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGAcgcgc                                                                                                                                 >  2:287901/1‑150 (MQ=255)

                ccGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTg                                                                                                                        <  1:180148/151‑1 (MQ=255)

                ccGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCGGGTGCTGTCGGTCGCGTGCAGTGGTAAGACGCTCGTACTgg                                                                                                                          <  1:177441/149‑1 (MQ=255)

                   cccAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGc                                                                                                                       >  1:184771/1‑149 (MQ=255)

                     cAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCg                                                                                                                   <  1:582371/151‑1 (MQ=255)

                        ccTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTAt                                                                                                                <  2:382443/151‑1 (MQ=255)

                           aTTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGGAAGACGCGCGTACTGGTGCATCGTATc                                                                                                               >  1:760965/1‑149 (MQ=255)

                                  cgcgGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTtg                                                                                                      <  2:15546/151‑1 (MQ=255)

                                   gcggcgGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTtga                                                                                                     <  2:830316/151‑1 (MQ=255)

                                              aaTGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTgg                                                                                            >  2:74732/1‑149 (MQ=255)

                                              aaTGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGaa                                                                                          <  2:45054/151‑1 (MQ=255)

                                                 ggACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGaaaa                                                                                        <  2:691184/150‑1 (MQ=255)

                                                  gACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACt                                                                                      <  1:817633/151‑1 (MQ=255)

                                                      tttCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTc                                                                                  >  2:282502/1‑151 (MQ=255)

                                                       ttCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCCCTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTc                                                                                  >  1:819784/1‑150 (MQ=255)

                                                              cTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGGGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATAc                                                                           >  1:615092/1‑150 (MQ=255)

                                                               tGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTc                                                                         >  2:159486/1‑151 (MQ=255)

                                                                gCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACt                                                                          <  1:480553/149‑1 (MQ=255)

                                                                 cTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGGACTGGTGCATCGTATCGCCTGGTTGATGAGCGGGGAAAACTGCTCGCCATACTCg                                                                        >  1:733674/1‑150 (MQ=255)

                                                                  tCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGGTGATGAGCGTGGAAAACTGCTCGCCATACTCGAt                                                                      >  1:513823/1‑151 (MQ=255)

                                                                   cGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGAt                                                                      <  1:177396/150‑1 (MQ=255)

                                                                       aGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATgg                                                                 <  2:567816/151‑1 (MQ=255)

                                                                        gCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGc                                                                <  2:281240/151‑1 (MQ=255)

                                                                         ccTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGGAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAAATGCTCGCCATACTCGATTATGGCg                                                               >  2:637100/1‑151 (MQ=255)

                                                                             aaTGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTG‑a                                                          <  2:796157/151‑1 (MQ=255)

                                                                                      cAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGGAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCggg                                                             >  1:122876/1‑139 (MQ=255)

                                                                                          gcgAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTG‑ACGTTTACcaacaa                                             <  2:531472/151‑1 (MQ=255)

                                                                                             aaGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTG‑ACGTTTACCAACAAAgc                                          <  1:246082/151‑1 (MQ=255)

                                                                                             aaGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCCCGCCATACTCGATTATGGCGGTG‑ACGTTTACCAACAAAgc                                          <  2:697429/151‑1 (MQ=255)

                                                                                                cGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGGGAAGGTTTTCCaaaaaaa                                           >  2:329980/1‑147 (MQ=255)

                                                                                                   tGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGTTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGCG‑AAGTTTTCcaaacaaaagg                                        >  2:161341/1‑139 (MQ=255)

                                                                                                   tGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGGAAGACGCGCGTACTGGTGCATCGTATCGCCTGGGTGGTGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTG‑GCGTTTTACTAAAAAacggcgg                                     >  1:134311/1‑150 (MQ=255)

                                                                                                       cgcgCCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTG‑ACGTTTACCAACAAAGCGGCGGCGgag                                <  1:215868/151‑1 (MQ=255)

                                                                                                        gcgcCACGCAGCAACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTG‑ACGTTTACCAACAAAGCGGCGGCGgaga                               <  2:28512/151‑1 (MQ=255)

                                                                                                                  gcaACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTG‑ACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCg                     <  1:113822/151‑1 (MQ=255)

                                                                                                                   caACCTTCTGGTGCTGGCGGTCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGGG‑GAGGTTTCCAACAAAAaggcggcgggg                                >  2:727435/1‑137 (MQ=255)

                                                                                                                                    cggtcgcgggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGGG‑GCGGTTTCCAAAAAAACCGCGGCGGAGGTGGGGCATCGTATCGGGGCAACT‑ATggg  >  1:156534/1‑151 (MQ=255)

AGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTG‑ACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATC‑GGGCAACTGATGGG  >  NC_000913/3997936‑3998218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A