Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I2 R1 173 150.8 4954114 96.5% 4780720 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 771,344 T→C intergenic (+733/‑114) mngB → / → cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913771,3440TC100.0% 522.1 / NA 139intergenic (+733/‑114)mngB/cydAalpha‑mannosidase/cytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (67/72);  total (67/72)

Only 100 of 145 total aligned reads displayed.

TTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCGCGCTTACAGT  >  NC_000913/771196‑771491
                                                                                                                                                    |                                                                                                                                                   
ttAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTgcgc                                                                                                                                                   >  1:1335007/1‑151 (MQ=255)
 tAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTgcgc                                                                                                                                                   >  1:244541/1‑150 (MQ=255)
    cTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTc                                                                                                                                               >  1:1262223/1‑151 (MQ=255)
      ataAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTca                                                                                                                                              <  1:5989/150‑1 (MQ=255)
        aaaTTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACAt                                                                                                                                           <  1:1827194/151‑1 (MQ=255)
          aTTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATgg                                                                                                                                         <  4:623537/151‑1 (MQ=255)
                cACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTatgat                                                                                                                                   <  1:1624258/151‑1 (MQ=255)
                  ccGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTAtgatga                                                                                                                                 <  1:932060/151‑1 (MQ=255)
                  ccGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTAtgatga                                                                                                                                 <  4:685167/151‑1 (MQ=255)
                   cGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTAtgatga                                                                                                                                 >  4:1249778/1‑150 (MQ=255)
                      cGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAg                                                                                                                              <  1:1917651/150‑1 (MQ=255)
                      cGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAg                                                                                                                              <  4:629508/150‑1 (MQ=255)
                         aaaaTGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGtt                                                                                                                          >  4:197483/1‑151 (MQ=255)
                           aaTGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTc                                                                                                                         >  4:1871742/1‑150 (MQ=255)
                              gCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTcaaa                                                                                                                      <  1:140577/150‑1 (MQ=255)
                                aaaTTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTcaaacaa                                                                                                                   <  1:1405814/151‑1 (MQ=255)
                                 aaTTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTcaaacaaa                                                                                                                  <  4:1254434/151‑1 (MQ=255)
                                   tttGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAAtt                                                                                                                >  4:405493/1‑151 (MQ=255)
                                     tGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAAttt                                                                                                               >  3:273148/1‑150 (MQ=255)
                                     tGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAAttt                                                                                                               <  4:699716/150‑1 (MQ=255)
                                      gCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCt                                                                                                             <  1:257128/151‑1 (MQ=255)
                                         tCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTAt                                                                                                           <  1:948739/150‑1 (MQ=255)
                                           aacaaAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTgg                                                                                                        >  2:489367/1‑151 (MQ=255)
                                            acaaAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTggg                                                                                                       <  3:323740/151‑1 (MQ=255)
                                            acaaAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTg                                                                                                         >  3:402873/1‑149 (MQ=255)
                                             caaAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTgggg                                                                                                      <  4:431671/151‑1 (MQ=255)
                                                   ccTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCg                                                                                                <  1:1287201/151‑1 (MQ=255)
                                                   ccTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTATTCAAACAAATTTCTATTGGGGCATGCg                                                                                                >  1:1905625/1‑151 (MQ=255)
                                                      gTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCgtg                                                                                              >  4:3131/1‑150 (MQ=255)
                                                      gTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCgt                                                                                               <  2:268768/149‑1 (MQ=255)
                                                          aTTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGAcc                                                                                         >  3:293318/1‑151 (MQ=255)
                                                           ttGTAAGTATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCAGGCGTGTGAcc                                                                                         <  1:1066457/150‑1 (MQ=255)
                                                             gTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCt                                                                                       <  1:1056454/150‑1 (MQ=255)
                                                                aGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCt                                                                                   <  4:893854/151‑1 (MQ=255)
                                                                   aTTTTGCGGCGTAATATATACGTGAGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAAc                                                                                >  3:11193/1‑151 (MQ=255)
                                                                     tttGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACgg                                                                              >  3:284267/1‑151 (MQ=255)
                                                                      ttGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACgg                                                                              <  1:1813840/150‑1 (MQ=255)
                                                                        gcggcgTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGt                                                                           <  1:1585770/151‑1 (MQ=255)
                                                                           gcgTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCa                                                                        <  1:1765867/151‑1 (MQ=255)
                                                                             gTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCAct                                                                      <  1:686114/151‑1 (MQ=255)
                                                                               aaTATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCActct                                                                    <  4:587489/151‑1 (MQ=255)
                                                                                 tatataCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCg                                                                  >  1:536109/1‑151 (MQ=255)
                                                                                  atataCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCgg                                                                 >  4:584821/1‑151 (MQ=255)
                                                                                    ataCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCgg                                                                 >  4:1734192/1‑149 (MQ=255)
                                                                                    ataCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCgg                                                                 >  1:1239166/1‑149 (MQ=255)
                                                                                      aCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGt                                                              <  1:1679345/150‑1 (MQ=255)
                                                                                       cGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCt                                                            <  4:1301965/151‑1 (MQ=255)
                                                                                         tGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTc                                                          >  3:326264/1‑151 (MQ=255)
                                                                                           ggATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCAt                                                        >  4:290771/1‑151 (MQ=255)
                                                                                           ggATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCAt                                                        <  2:366954/151‑1 (MQ=255)
                                                                                           ggATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCAt                                                        >  2:155016/1‑151 (MQ=255)
                                                                                            gATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATg                                                       >  1:694408/1‑151 (MQ=255)
                                                                                               cAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGc                                                      <  4:455643/149‑1 (MQ=255)
                                                                                               cAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTAAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCg                                                     >  1:360123/1‑150 (MQ=255)
                                                                                                aaTTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCGTGCGAt                                                   <  4:1476117/151‑1 (MQ=255)
                                                                                                aaTTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGAt                                                   >  4:289761/1‑151 (MQ=255)
                                                                                                aaTTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGAt                                                   >  1:1438705/1‑151 (MQ=255)
                                                                                                 aTTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATg                                                  <  4:194960/151‑1 (MQ=255)
                                                                                                   ttGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGa                                                 <  3:254159/150‑1 (MQ=255)
                                                                                                    tGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAg                                                <  1:240964/150‑1 (MQ=255)
                                                                                                    tGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGc                                               >  3:310692/1‑151 (MQ=255)
                                                                                                     gAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGc                                               >  1:1181531/1‑150 (MQ=255)
                                                                                                     gAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCa                                              <  1:291636/151‑1 (MQ=255)
                                                                                                        tttttATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAgg                                           >  2:220566/1‑151 (MQ=255)
                                                                                                        tttttATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAgg                                           >  1:923746/1‑151 (MQ=255)
                                                                                                          tttATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAgg                                           >  4:775229/1‑149 (MQ=255)
                                                                                                            tattaACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGt                                        <  1:1601178/150‑1 (MQ=255)
                                                                                                            tattaACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGt                                        <  1:832279/150‑1 (MQ=255)
                                                                                                            tattaACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTc                                       >  4:1480502/1‑151 (MQ=255)
                                                                                                            tattaACATGTTTGCAACCTTTCTGTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGt                                        <  3:452754/150‑1 (MQ=255)
                                                                                                                aaCATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCatga                                   <  1:589840/151‑1 (MQ=255)
                                                                                                                aaCATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCatg                                    >  4:848505/1‑150 (MQ=255)
                                                                                                                  cATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCatgatg                                 <  4:1331645/151‑1 (MQ=255)
                                                                                                                    tGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGtt                               >  1:637009/1‑151 (MQ=255)
                                                                                                                    tGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGtt                               >  2:373870/1‑151 (MQ=255)
                                                                                                                     gTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTa                              <  2:288856/151‑1 (MQ=255)
                                                                                                                       ttGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAg                             <  4:1785050/150‑1 (MQ=255)
                                                                                                                       ttGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAg                             <  4:321689/150‑1 (MQ=255)
                                                                                                                        tGCAGCCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGtt                               >  1:332083/1‑147 (MQ=255)
                                                                                                                           aaCCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGatat                         >  3:161043/1‑150 (MQ=255)
                                                                                                                             ccTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGt                      >  4:1689597/1‑151 (MQ=255)
                                                                                                                             ccTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGt                      <  4:595908/151‑1 (MQ=255)
                                                                                                                             ccTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGt                      >  4:535741/1‑151 (MQ=255)
                                                                                                                                ttctttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTc                     <  1:1572101/149‑1 (MQ=255)
                                                                                                                                 tctttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGa                   >  4:1728050/1‑150 (MQ=255)
                                                                                                                                 tctttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGa                   <  3:188024/150‑1 (MQ=255)
                                                                                                                                   tttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACt                >  3:39274/1‑151 (MQ=255)
                                                                                                                                   tttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACt                >  4:26428/1‑151 (MQ=255)
                                                                                                                                   tttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACt                >  1:1635924/1‑151 (MQ=255)
                                                                                                                                    ttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGaa                  <  4:150939/148‑1 (MQ=255)
                                                                                                                                    ttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAAc                 <  1:1791134/149‑1 (MQ=255)
                                                                                                                                    ttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAAc                 <  1:1733514/149‑1 (MQ=255)
                                                                                                                                      aCGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTc             <  1:813099/151‑1 (MQ=255)
                                                                                                                                       cGCCGTTTTTGTGCGCATTCGCATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTcg            <  4:1664463/151‑1 (MQ=255)
                                                                                                                                       cGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTcg            <  1:382969/151‑1 (MQ=255)
                                                                                                                                          cGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCgcgc         <  3:388597/151‑1 (MQ=255)
                                                                                                                                             ttttGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCGCGCtt       >  3:495853/1‑150 (MQ=255)
                                                                                                                                                 gtgcgCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCGCGCTTACAGt  <  1:1326736/151‑1 (MQ=255)
                                                                                                                                                 gtgcgCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCGCGCTTACAGt  <  4:887633/151‑1 (MQ=255)
                                                                                                                                                 gtgcgCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCGCGCTTACAGt  >  1:1035035/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCGCGCTTACAGT  >  NC_000913/771196‑771491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A