Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	771,344	T→C	intergenic (+733/‑114)	mngB → / → cydA	alpha‑mannosidase/cytochrome d terminal oxidase, subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	771,344	0	T	C	100.0%	93.9 / NA	27	intergenic (+733/‑114)	mngB/cydA	alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/10); total (17/10)

GATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCGCGCTTACA  >  NC_000913/771209‑771489

gATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTat                                                                                                                                    <  1:484227/151‑1 (MQ=255)

gATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTa                                                                                                                                     <  2:143687/150‑1 (MQ=255)

       gTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGaaa                                                                                                                             <  2:433229/151‑1 (MQ=255)

        tCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGaa                                                                                                                              >  2:55931/1‑149 (MQ=255)

         cGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAgt                                                                                                                           <  2:556354/151‑1 (MQ=255)

          gAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAgtg                                                                                                                          <  2:123451/151‑1 (MQ=255)

            aaaaTGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGtt                                                                                                                        >  2:12873/1‑151 (MQ=255)

                   aaaTTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTcaaaca                                                                                                                  >  1:134178/1‑150 (MQ=255)

                       ttGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAAttt                                                                                                             >  1:443326/1‑151 (MQ=255)

                            tCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTAtt                                                                                                        >  1:75139/1‑151 (MQ=255)

                                    aaCCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATg                                                                                                >  2:552047/1‑151 (MQ=255)

                                           ttATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGtgtg                                                                                          >  1:584328/1‑150 (MQ=255)

                                            tATTGCAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGAc                                                                                        >  2:41448/1‑151 (MQ=255)

                                                  aaGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTc                                                                                  <  1:179064/151‑1 (MQ=255)

                                                  aaGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTc                                                                                  >  1:357534/1‑151 (MQ=255)

                                                         ttGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACggg                                                                           >  1:461138/1‑151 (MQ=255)

                                                                         aCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGt                                                            <  2:514169/150‑1 (MQ=255)

                                                                               gATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATg                                                     <  2:452616/151‑1 (MQ=255)

                                                                                   aaTTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGAt                                                 >  1:497334/1‑151 (MQ=255)

                                                                                          gTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCa                                            >  1:569343/1‑149 (MQ=255)

                                                                                            ttttATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAgg                                         >  2:296158/1‑150 (MQ=255)

                                                                                                    aCATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCatgat                                >  1:44988/1‑151 (MQ=255)

                                                                                                                   ttctttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTc                   <  2:492895/149‑1 (MQ=255)

                                                                                                                      tttACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACt              >  2:494693/1‑151 (MQ=255)

                                                                                                                        tACGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGt            >  2:574893/1‑151 (MQ=255)

                                                                                                                         aCGCCGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTc           <  2:443318/151‑1 (MQ=255)

                                                                                                                            ccGTTTTTGTGCGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTcgcg        >  2:374301/1‑151 (MQ=255)

                                                                                                                                   tgtgcgCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCGCGCTTACa  <  2:525506/150‑1 (MQ=255)

GATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAACGGGGTTCACTCTCGGAGTCTTCATGCGATGAGCAAGGAGTCATGATGTTAGATATAGTCGAACTGTCGCGCTTACA  >  NC_000913/771209‑771489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A