Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I2 R1 173 150.8 4954114 96.5% 4780720 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,098,260 G→A D14D (GAC→GAT)  wzzB ← regulator of length of O‑antigen component of lipopolysaccharide chains

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,098,2600GA99.4% 707.7 / ‑3.8 175D14D (GAC→GAT) wzzBregulator of length of O‑antigen component of lipopolysaccharide chains
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (79/95);  minor base T (0/1);  total (79/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.58e-01

Only 100 of 175 total aligned reads displayed.

AGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTGTAAGTAAC  >  NC_000913/2098113‑2098406
                                                                                                                                                   |                                                                                                                                                  
aGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATc                                                                                                                                                  >  1:692246/1‑150 (MQ=255)
 gTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCAt                                                                                                                                                >  1:1103611/1‑151 (MQ=255)
  tGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCAt                                                                                                                                                <  4:835842/150‑1 (MQ=255)
    aTAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGt                                                                                                                                             >  4:228252/1‑151 (MQ=255)
     tAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGtt                                                                                                                                            >  4:938041/1‑151 (MQ=255)
     tAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGtt                                                                                                                                            >  4:713301/1‑151 (MQ=255)
     tAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGtt                                                                                                                                            >  1:3207/1‑151 (MQ=255)
      aaTTGCTGTTGACGTCCATTTCTCCTCCGCTACTGCAAAATATCCAATAGCCAGGGAAATAGCCAAAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATg                                                                                                                                               <  1:1450591/147‑1 (MQ=255)
      aaTTGCTGTTGACGTCCATTTCCCCTTCGCTACTGCAAAAAATCCAAGAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCAt                                                                                                                                                <  2:179401/146‑1 (MQ=255)
          gCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGcc                                                                                                                                       >  4:494658/1‑151 (MQ=255)
             gtggACGTCCATTTCCCCTTCGCTACTGCAAAATATCCAATAGCCAGGGCAATAGCCAAAAGAACGGAAATGATGATTGCCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCa                                                                                                                                     <  4:1337975/147‑1 (MQ=255)
              ttGCCGTCCATTTCTCCTTCGCTACTGCCAAATACCCAATAGCCAGGGCAATAGCCACAATGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAg                                                                                                                                    <  4:254397/150‑1 (MQ=255)
              ttGACGTCCATTTCTCCTTCGCTACTGCAAAATATCAAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAg                                                                                                                                    <  4:758560/150‑1 (MQ=255)
              tggACGTCCATTTCTCCTCCGCTACTGCAAAATACCCAATAGCCAGGGCAATAGCCACAATGACGAAAATGAGGTTTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAg                                                                                                                                    <  1:1228469/148‑1 (MQ=255)
                gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGaa                                                                                                                                  >  4:1720613/1‑150 (MQ=255)
                gACGTCATTTTCTCCTTCGCTACTGCCAAACACCCAAAACCCGGGGAAATACCCAAAATGGCGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGaaa                                                                                                                                 <  4:529268/151‑1 (MQ=255)
                 aCGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAAc                                                                                                                                >  1:395098/1‑151 (MQ=255)
                 aCGTCCATTTCTCCTTCGCTACTGCCAAATACCCAATAGCCAGGGCAATAGCCACAATGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGaa                                                                                                                                  <  1:1210963/149‑1 (MQ=255)
                    tCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACat                                                                                                                              <  4:224262/150‑1 (MQ=255)
                           ctcCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTAttt                                                                                                                      >  3:468009/1‑151 (MQ=255)
                           ctcCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTAttt                                                                                                                      >  4:508297/1‑151 (MQ=255)
                           ctcCTTCGCTACTGCCAAATATCCAATAGCCAGGGAAATAGCCACAATGACGGAAATGATGATTGTCATTTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACattattat                                                                                                                        <  2:351961/149‑1 (MQ=255)
                             ccTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTc                                                                                                                    >  1:1069602/1‑151 (MQ=255)
                             ccTACGCTCCGGCAAAATATCCAAGACCCAGGGCAATAGCCAAAAGGACGAAAAGGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTAttt                                                                                                                      <  2:311833/149‑1 (MQ=255)
                               ttCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCt                                                                                                                   >  1:1254882/1‑150 (MQ=255)
                                  ccaaCTGCAAAAAATAAAATAGCCGGGCCAATAGCCCGAAGGCGGGAAATGATAGTTGTCTTCTTGCCACGCCACAACTGCACGAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACattattatt                                                                                                                       <  3:82476/140‑1 (MQ=255)
                                    tACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACtctc                                                                                                             >  1:1777182/1‑151 (MQ=255)
                                    tACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACtctc                                                                                                             >  2:416944/1‑151 (MQ=255)
                                     aCTGCCAAATACCCAATAGCCAGGGAAATGGCAAAAATGCCGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTActct                                                                                                              <  4:234745/149‑1 (MQ=255)
                                           aaaTTTCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCtcat                                                                                                           <  1:1415673/146‑1 (MQ=255)
                                           aaaTATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCtcat                                                                                                           <  4:378103/146‑1 (MQ=255)
                                           aaaTATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCAtt                                                                                                       >  4:1563952/1‑150 (MQ=255)
                                           aaaTATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCAtt                                                                                                       >  1:424940/1‑150 (MQ=255)
                                           aaaTATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTa                                                                                                      <  4:810977/151‑1 (MQ=255)
                                           aaaTACCCAATAGCCAGGGCAATAGCCACAATGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCtca                                                                                                            <  4:1064343/145‑1 (MQ=255)
                                            aaTACCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTAc                                                                                                     <  4:169412/151‑1 (MQ=255)
                                             atatCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTAcc                                                                                                    >  3:52918/1‑151 (MQ=255)
                                               atCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCt                                                                                                  >  2:91278/1‑151 (MQ=255)
                                                tCCAATAGCCAGGGCAATAGCCACAATGGCGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCt                                                                                                  >  1:945318/1‑150 (MQ=255)
                                                   aaTAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACt                                                                                              >  1:1527728/1‑151 (MQ=255)
                                                      aGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGAc                                                                                           >  4:1383886/1‑151 (MQ=255)
                                                         cAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACgg                                                                                         >  4:1008435/1‑150 (MQ=255)
                                                           gggAAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGa                                                                                        <  4:1250803/149‑1 (MQ=255)
                                                            ggCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAAt                                                                                     <  1:537643/151‑1 (MQ=255)
                                                               aaTAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAAttt                                                                                   <  4:1844852/150‑1 (MQ=255)
                                                               aaTAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTc                                                                                  <  1:934227/151‑1 (MQ=255)
                                                               aaTAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTc                                                                                  >  4:180092/1‑151 (MQ=255)
                                                               aaTAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTc                                                                                  >  1:1158092/1‑151 (MQ=255)
                                                                aTAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTcc                                                                                 >  4:1310709/1‑151 (MQ=255)
                                                                 tAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTcc                                                                                 >  1:1282129/1‑150 (MQ=255)
                                                                  aGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCa                                                                                >  1:1640717/1‑150 (MQ=255)
                                                                    ccACAATGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGt                                                                             <  4:195332/151‑1 (MQ=255)
                                                                      acaATGACGGAAATGATGTTTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCaa                                                                               <  4:477692/147‑1 (MQ=255)
                                                                        aaTGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCg                                                                          >  4:1187509/1‑150 (MQ=255)
                                                                        aaTGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGc                                                                         >  4:558940/1‑151 (MQ=255)
                                                                          tGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCAt                                                                       >  2:511774/1‑151 (MQ=255)
                                                                             cGGAAATGATGATTGTCATCTCGCCACGCCGCACCTCCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAg                                                                     >  2:102891/1‑150 (MQ=255)
                                                                                aaaTGATGTTTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGt                                                                    <  1:22302/148‑1 (MQ=255)
                                                                                aaaTGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGttt                                                                  >  4:520921/1‑150 (MQ=255)
                                                                                aaaTGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTa                                                                 <  3:151070/151‑1 (MQ=255)
                                                                                aaaTGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTa                                                                 <  2:468025/151‑1 (MQ=255)
                                                                                          tGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATcc                                                       >  1:577598/1‑151 (MQ=255)
                                                                                             cATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAg                                                                     >  3:400302/1‑134 (MQ=255)
                                                                                             cATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCt                                                      <  4:144649/149‑1 (MQ=255)
                                                                                              aTCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGt                                                   >  4:1535882/1‑151 (MQ=255)
                                                                                               tCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTg                                                  >  3:103636/1‑151 (MQ=255)
                                                                                                   gccacgccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGaa                                                <  4:1162766/149‑1 (MQ=255)
                                                                                                    ccacgccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGa                                                 <  1:1719172/147‑1 (MQ=255)
                                                                                                    ccacgccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAAt                                               <  3:340141/149‑1 (MQ=255)
                                                                                                    ccacgccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGc                                             >  1:30642/1‑151 (MQ=255)
                                                                                                      acgccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCta                                           <  3:244583/151‑1 (MQ=255)
                                                                                                       cgccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCtat                                          >  1:1854130/1‑151 (MQ=255)
                                                                                                         ccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAg                                        <  2:142682/151‑1 (MQ=255)
                                                                                                          cacaACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAgg                                       >  4:1573108/1‑151 (MQ=255)
                                                                                                              aCTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATaa                                   <  1:655492/151‑1 (MQ=255)
                                                                                                                 gCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTg                                <  4:680904/151‑1 (MQ=255)
                                                                                                                      aGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATaaa                           <  4:665690/151‑1 (MQ=255)
                                                                                                                      aGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATaaa                           <  4:1663550/151‑1 (MQ=255)
                                                                                                                      aGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATa                             <  4:1047366/149‑1 (MQ=255)
                                                                                                                       gTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATaaaa                          <  3:121357/151‑1 (MQ=255)
                                                                                                                           atcaatcaaatcaatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAaata                       >  4:1481960/1‑150 (MQ=255)
                                                                                                                             caatcaaatcaatcTGTTCCGGTTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATg                    <  4:700048/151‑1 (MQ=255)
                                                                                                                               atcaaatcaatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGa                   >  1:1645701/1‑150 (MQ=255)
                                                                                                                                 caaatcaatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGGATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAAtt                >  4:110404/1‑151 (MQ=255)
                                                                                                                                  aaatcaatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAAttg               >  4:360344/1‑151 (MQ=255)
                                                                                                                                   aatcaatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAAttgt              <  1:1917771/151‑1 (MQ=255)
                                                                                                                                    atcaatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAAttgtt             <  2:222981/151‑1 (MQ=255)
                                                                                                                                    atcaatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAAttgtt             <  1:362803/151‑1 (MQ=255)
                                                                                                                                      caatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATtgttgt           <  1:18966/151‑1 (MQ=255)
                                                                                                                                      caatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATtgttgt           <  4:725737/151‑1 (MQ=255)
                                                                                                                                           tGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTgta        >  4:1272202/1‑149 (MQ=255)
                                                                                                                                            gTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTgtgt         <  4:674653/147‑1 (MQ=255)
                                                                                                                                            gTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTgtaag      <  1:1532310/150‑1 (MQ=255)
                                                                                                                                            gTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTgtaag      <  1:712988/150‑1 (MQ=255)
                                                                                                                                            gTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTgtaa       <  1:817659/149‑1 (MQ=255)
                                                                                                                                            gTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTgtaa       <  1:1119750/149‑1 (MQ=255)
                                                                                                                                            gTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTgta        <  4:710875/148‑1 (MQ=255)
                                                                                                                                               ccGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTGTAAGTAAc  <  1:1856645/151‑1 (MQ=255)
                                                                                                                                               ccGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTGTAAGTAAc  >  3:365938/1‑151 (MQ=255)
                                                                                                                                                cGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTGTAAGTAAc  >  4:878340/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTGTAAGTAAC  >  NC_000913/2098113‑2098406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A