Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F50 I1 R1
|
170 |
39.5 |
1266514 |
99.2% |
1256381 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,098,260 |
G→A |
D14D (GAC→GAT) |
wzzB ← |
regulator of length of O‑antigen component of lipopolysaccharide chains |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,098,260 | 0 | G | A | 100.0%
| 133.6
/ NA
| 35 | D14D (GAC→GAT) | wzzB | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/24); total (11/24) |
GTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTGTAAGTAACA > NC_000913/2098114‑2098407
|
gTGATAATTGCTGTTGCGGTCCATTTCTCCTTCGCTACTGCAAAATACCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGTTTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCAt < 1:307386/151‑1 (MQ=255)
aTAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCAAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATg < 1:365508/149‑1 (MQ=255)
aaTTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGttt > 1:573932/1‑151 (MQ=255)
tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACattatt < 1:593396/150‑1 (MQ=255)
tttCTCCTTCGCTACTGCAAAATATCCAATAGCCAGGGCAATAGCCACAATGACGAAAATGATGATTGTCTTCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACatt < 2:337498/147‑1 (MQ=255)
tttCTCCTCCGCTACGGCAAAATACCCAATAGCCAGGGCAATAGCCAAAATGACGAAAATGATGATTGTCATTTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACatt < 2:620702/147‑1 (MQ=255)
ttCTCCTTCGCTACTGCAAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACattatta < 2:232811/150‑1 (MQ=255)
cGCTACTGCCAAATACCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTAc < 1:219576/150‑1 (MQ=255)
ccAAATATCAAATAGCCAGGACAATACCCAAAAGTACTAAAATAATGATTCTCATTTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCtcatc < 2:549644/149‑1 (MQ=255)
aaaTATCCAATAGCCAGGGCAATAGCCACAATGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCAtt < 2:574581/150‑1 (MQ=255)
aaaTATCCAATAGCCAGGGCAATAGCCAAAATGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTa < 1:387089/151‑1 (MQ=255)
tatCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTAccc > 2:603210/1‑151 (MQ=255)
ccAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTa > 1:120086/1‑150 (MQ=255)
tAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTg > 2:456211/1‑150 (MQ=255)
aTAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTcc > 1:360022/1‑151 (MQ=255)
tGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCAt > 2:627632/1‑151 (MQ=255)
ggAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGtt < 2:400198/151‑1 (MQ=255)
aaaTGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTa < 1:514982/151‑1 (MQ=255)
tgatgaTTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCg > 1:73232/1‑151 (MQ=255)
ttGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAAt < 1:175296/150‑1 (MQ=255)
tCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTc < 1:541999/148‑1 (MQ=255)
ttGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGa < 1:96195/150‑1 (MQ=255)
ccacgccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATg < 1:133832/150‑1 (MQ=255)
cacgccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCt > 1:280319/1‑151 (MQ=255)
gccacAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCtat > 2:264544/1‑150 (MQ=255)
acaACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAgg > 1:592104/1‑150 (MQ=255)
acaACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGa < 1:163715/151‑1 (MQ=255)
aCTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATaa < 1:580427/151‑1 (MQ=255)
gCACTAGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGt < 1:339737/150‑1 (MQ=255)
aGTAAATCAATCAAATCAATCTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATaa > 2:404365/1‑150 (MQ=255)
caatcaaatcaatcTGTTCCGGATCATGGTTTTGCTCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACAGAAATTTCCAAGTCCGCATAGTATAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAaataat < 2:901/150‑1 (MQ=255)
caatcaaatcaatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATg < 1:603225/151‑1 (MQ=255)
caaatcaatcTGTTCCGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAAtt < 2:123060/151‑1 (MQ=255)
aatcaatcTGTGCCGGATCATGGTTTTGCGCAGAAACATTATTAGTTTCTACTCTCAGCATTACCCGAACTGGGGGGAATTTGGAGGGGCGCATAGGGTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAAttgt < 2:357854/151‑1 (MQ=255)
cGGATCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTGTAAGTAACa < 1:477600/151‑1 (MQ=255)
|
GTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAAGTGATAAAAATAATGAATTGTTGTGTAAGTAACA > NC_000913/2098114‑2098407
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
