Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I2 R1 173 150.8 4954114 96.5% 4780720 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,441,814 T→C G17G (GGT→GGC)  mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,441,8140TC99.4% 549.2 / ‑4.1 155G17G (GGT→GGC) mnmCfused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (63/91);  total (64/91)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

Only 100 of 156 total aligned reads displayed.

CGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAG  >  NC_000913/2441667‑2441957
                                                                                                                                                   |                                                                                                                                                
cGCCGAACAATTCCGATCAGCCATTTAATAGAAAAATTTGCGCAGCCTTACACACATCGCTAAGATGGGCCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCac                                                                                                                                                <  4:901329/150‑1 (MQ=255)
cGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCac                                                                                                                                                >  3:91583/1‑150 (MQ=255)
cGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCac                                                                                                                                                <  4:1564272/150‑1 (MQ=255)
cGCCGAACAAGTCCGATCAGCCATTTAATAGAAAAATTTGCGCAGCCTTACCCACATCTCTAAGACCGCCCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCac                                                                                                                                                <  3:375848/150‑1 (MQ=255)
    gAACAGGCCCGACCAGCCATTTAATAGACAAATTTGCGCACCCTTACCCACATCCATAAGATCGAGCCCCCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCt                                                                                                                                            <  1:563282/150‑1 (MQ=255)
    gAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTg                                                                                                                                           >  4:542746/1‑151 (MQ=255)
     aaCAAGTCCGATCGGCCTTTTAATAGAGAAATTTGCGCGGCCTTACACACATCGCTAAGATCGACCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGt                                                                                                                                          <  3:437784/151‑1 (MQ=255)
      aCAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGtt                                                                                                                                         >  3:203235/1‑151 (MQ=255)
        aaGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTc                                                                                                                                       <  4:704303/151‑1 (MQ=255)
        aaGCCCGATCAGCCATTTAAAAGAAAAATTTGCCCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTc                                                                                                                                       <  4:1162618/151‑1 (MQ=255)
         aGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTcc                                                                                                                                      >  2:79966/1‑151 (MQ=255)
           tCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCATCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCg                                                                                                                                    >  1:219338/1‑151 (MQ=255)
           tCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCg                                                                                                                                    >  2:360043/1‑151 (MQ=255)
                 cAGCCATTTAATAGAGAAATTTGCGCACCCTTACACACATCGCAAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGAtt                                                                                                                               <  4:629050/150‑1 (MQ=255)
                  aGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGAtttt                                                                                                                             >  4:249688/1‑151 (MQ=255)
                  aGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGAtttt                                                                                                                             >  2:211014/1‑151 (MQ=255)
                    ccATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGAtttt                                                                                                                             <  1:794625/149‑1 (MQ=255)
                     cATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGAc                                                                                                                          >  1:1230219/1‑151 (MQ=255)
                     cATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCAAAAATCGACCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGAtttt                                                                                                                             <  1:40831/148‑1 (MQ=255)
                       tttAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACg                                                                                                                         >  3:444906/1‑150 (MQ=255)
                       tttAATAGAAAATTTTGCGCAGCCTTACACACATCGCTAAGATCGACCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACg                                                                                                                         <  1:1022132/150‑1 (MQ=255)
                        ttAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGa                                                                                                                        <  1:1037025/150‑1 (MQ=255)
                        ttAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGAt                                                                                                                       >  1:193770/1‑151 (MQ=255)
                           aTAGAGAAATTTGCGCAGCCTTACCCACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTc                                                                                                                    <  4:296855/151‑1 (MQ=255)
                           aTAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTc                                                                                                                    >  4:1338800/1‑151 (MQ=255)
                             agagAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCt                                                                                                                   <  2:194950/150‑1 (MQ=255)
                               agaAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTAtt                                                                                                                >  4:925374/1‑151 (MQ=255)
                               agaAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTAtt                                                                                                                >  4:1227247/1‑151 (MQ=255)
                               agaAATTTGCGCAGCCTTACACACATCCCTAAGACCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTAtt                                                                                                                <  4:120826/151‑1 (MQ=255)
                                 aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTAtttt                                                                                                              >  3:438900/1‑151 (MQ=255)
                                 aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTAtttt                                                                                                              <  1:676177/151‑1 (MQ=255)
                                  aaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTAttttt                                                                                                             <  4:1479704/151‑1 (MQ=255)
                                      tGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCa                                                                                                          <  1:977698/150‑1 (MQ=255)
                                      tGCGCAGCCTAACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCa                                                                                                          <  1:361672/150‑1 (MQ=255)
                                       gcgcAGCCTTACACACATCGCAAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCa                                                                                                          <  4:1895897/149‑1 (MQ=255)
                                          cAGCCTTACACACATCGCTAATATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GAt                                                                                                    <  2:128892/151‑1 (MQ=255)
                                          cAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GAt                                                                                                    >  2:500025/1‑151 (MQ=255)
                                           aGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATa                                                                                                   <  2:410642/151‑1 (MQ=255)
                                             ccTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATa                                                                                                   <  4:768373/149‑1 (MQ=255)
                                             ccTTACACACATCGCAAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATaa                                                                                                  <  4:1584307/150‑1 (MQ=255)
                                              cTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACg                                                                                                >  2:185295/1‑151 (MQ=255)
                                                    cacaTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTgg                                                                                          >  2:240796/1‑151 (MQ=255)
                                                     acaTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGa                                                                                         <  4:138654/151‑1 (MQ=255)
                                                      caTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTgg                                                                                          <  4:222007/149‑1 (MQ=255)
                                                       aTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGa                                                                                         >  4:304226/1‑149 (MQ=255)
                                                         cGCTAAGATCGGGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAag                                                                                       <  4:1706974/149‑1 (MQ=255)
                                                         cGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGaa                                                                                        <  3:300124/148‑1 (MQ=255)
                                                         cGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAagag                                                                                     <  2:47610/151‑1 (MQ=255)
                                                         cGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAaga                                                                                      <  4:1669892/150‑1 (MQ=255)
                                                             aaGATCGGGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGAc                                                                                   <  1:130488/149‑1 (MQ=255)
                                                                  cGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTAt                                                                            >  1:1315394/1‑151 (MQ=255)
                                                                    acccACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTAt                                                                            <  1:1827122/147‑1 (MQ=255)
                                                                     gCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTAt                                                                            <  4:227507/148‑1 (MQ=255)
                                                                      ccACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGt                                                                          <  4:82894/149‑1 (MQ=255)
                                                                       cACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGt                                                                          <  4:456377/148‑1 (MQ=255)
                                                                       cACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTCTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGtttt                                                                       <  1:496701/151‑1 (MQ=255)
                                                                           gCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTg                                                                   <  4:1544498/151‑1 (MQ=255)
                                                                            ccTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTgg                                                                  <  4:1075303/151‑1 (MQ=255)
                                                                               gTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAAGGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTGTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGa                                                                <  3:111080/150‑1 (MQ=255)
                                                                                 aaGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAgg                                                              <  4:159529/150‑1 (MQ=255)
                                                                                  aGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGc                                                             >  1:1080517/1‑150 (MQ=255)
                                                                                    aCGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAAc                                                          >  1:1746845/1‑151 (MQ=255)
                                                                                       aGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCa                                                        >  1:1923065/1‑150 (MQ=255)
                                                                                         tAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAAtt                                                     >  4:1130756/1‑151 (MQ=255)
                                                                                           aCTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAAtt                                                     >  1:1182750/1‑149 (MQ=255)
                                                                                             ttACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTag                                                   >  1:262193/1‑149 (MQ=255)
                                                                                              tACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAgg                                                >  3:483802/1‑151 (MQ=255)
                                                                                               aCGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAgg                                                <  1:1722278/150‑1 (MQ=255)
                                                                                                 gTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTAc                                             >  4:1183792/1‑151 (MQ=255)
                                                                                                 gTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTAc                                             <  4:904131/151‑1 (MQ=255)
                                                                                                         ctactCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTc                                       <  1:1756727/149‑1 (MQ=255)
                                                                                                          tactCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTg                                    >  1:1402444/1‑151 (MQ=255)
                                                                                                           actCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTg                                    >  4:1637723/1‑150 (MQ=255)
                                                                                                           actCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGa                                   <  1:726161/151‑1 (MQ=255)
                                                                                                           actCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGa                                   <  4:456235/151‑1 (MQ=255)
                                                                                                            ctCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTg                                    >  3:342937/1‑149 (MQ=255)
                                                                                                            ctCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTg                                    >  1:1575663/1‑149 (MQ=255)
                                                                                                              ccATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAg                                  <  4:357576/149‑1 (MQ=255)
                                                                                                              cattaaacccTGCAACCCCCGAATTTAATGTGGAGGGCCCCCCTGTTCCCGGGGTTTTTGACGA‑GTCTAATTTTCCAACGGATACCGGGTTGGAAGAG‑CGCCGTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCAt                               <  1:1189305/143‑1 (MQ=255)
                                                                                                               cATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCAt                               <  1:606547/151‑1 (MQ=255)
                                                                                                                 tACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATcc                             >  1:412554/1‑151 (MQ=255)
                                                                                                                  aCAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCca                            <  1:903662/151‑1 (MQ=255)
                                                                                                                   cAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCcac                           <  1:517068/151‑1 (MQ=255)
                                                                                                                    aaCCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCcac                           >  1:337937/1‑150 (MQ=255)
                                                                                                                     aCCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACAt                         >  4:298755/1‑151 (MQ=255)
                                                                                                                      ccTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATc                        >  1:941413/1‑151 (MQ=255)
                                                                                                                        tGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCct                      >  4:1777204/1‑151 (MQ=255)
                                                                                                                         gCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCct                      <  4:734452/150‑1 (MQ=255)
                                                                                                                            aaCCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGt                  <  1:1616858/151‑1 (MQ=255)
                                                                                                                             aCCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGtt                 <  4:34702/151‑1 (MQ=255)
                                                                                                                              ccTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGttt                <  4:269381/151‑1 (MQ=255)
                                                                                                                                   aaTTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGt           <  4:228557/151‑1 (MQ=255)
                                                                                                                                      ttAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAAGTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTa          <  1:1478580/149‑1 (MQ=255)
                                                                                                                                       tAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGc        >  1:564755/1‑150 (MQ=255)
                                                                                                                                        aaTGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGc        >  1:1092100/1‑149 (MQ=255)
                                                                                                                                        aaTGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCa       <  3:53345/150‑1 (MQ=255)
                                                                                                                                         aTGCTGATGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGc        >  1:1686784/1‑148 (MQ=255)
                                                                                                                                         aTGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCaga     <  1:297690/151‑1 (MQ=255)
                                                                                                                                            cTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCagag    >  4:57044/1‑149 (MQ=255)
                                                                                                                                              gAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCagagag  <  1:816995/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAAC‑GATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAG  >  NC_000913/2441667‑2441957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A