Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,441,814	T→C	G17G (GGT→GGC)	mnmC →	fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,441,814	0	T	C	100.0%	127.6 / NA	36	G17G (GGT→GGC)	mnmC	fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/19); total (17/19)

ACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAG  >  NC_000913/2441666‑2441950

aCGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCac                                                                                                                                        >  1:187942/1‑151 (MQ=255)

   ccGAACAAGCCCGATCAGCCATTTAATAGAGAAATTTGCACAGCCTAACACACATCGCTAAGATCGACCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCac                                                                                                                                        <  2:73160/148‑1 (MQ=255)

     gAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTg                                                                                                                                   >  2:311347/1‑151 (MQ=255)

       aCAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGtt                                                                                                                                 >  1:191619/1‑151 (MQ=255)

         aaGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGttt                                                                                                                                <  1:124641/150‑1 (MQ=255)

                     ccATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGAtttt                                                                                                                     <  1:509926/149‑1 (MQ=255)

                          tAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGAt                                                                                                               <  1:484787/150‑1 (MQ=255)

                          tAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATg                                                                                                              >  2:121051/1‑151 (MQ=255)

                           aaTAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGt                                                                                                             >  1:293152/1‑151 (MQ=255)

                             tAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCt                                                                                                           >  1:175134/1‑151 (MQ=255)

                              agagAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCt                                                                                                           >  1:310055/1‑150 (MQ=255)

                               gagaAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTAt                                                                                                         >  1:628555/1‑151 (MQ=255)

                                   aaTTTGCGCAGCCTTACCCACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTAttttt                                                                                                     <  1:424188/151‑1 (MQ=255)

                                     tttGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTc                                                                                                    <  2:575254/150‑1 (MQ=255)

                                       tGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCa                                                                                                  >  2:569562/1‑150 (MQ=255)

                                       tGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCa                                                                                                  >  2:304186/1‑150 (MQ=255)

                                                      acaTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTgg                                                                                   >  1:199402/1‑150 (MQ=255)

                                                          cGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAagag                                                                              <  1:159948/151‑1 (MQ=255)

                                                                 aTCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGtt                                                                       >  2:249502/1‑151 (MQ=255)

                                                                       ccACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGttt                                                                 <  1:395665/151‑1 (MQ=255)

                                                                         aCCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGttttt                                                               <  1:195408/151‑1 (MQ=255)

                                                                         aCCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGtt                                                                  <  1:203713/148‑1 (MQ=255)

                                                                            gCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTg                                                            >  2:447785/1‑151 (MQ=255)

                                                                                gTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTggg                                                          <  1:158722/149‑1 (MQ=255)

                                                                                     aCGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAAc                                                   >  2:436958/1‑151 (MQ=255)

                                                                                                aCGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGt                                        <  1:369968/151‑1 (MQ=255)

                                                                                                                   aCAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATc                       >  2:189607/1‑149 (MQ=255)

                                                                                                                   aCAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCca                     <  2:323902/151‑1 (MQ=255)

                                                                                                                    cAACCTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCcac                    <  2:532417/151‑1 (MQ=255)

                                                                                                                       ccTGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATc                 <  1:404618/151‑1 (MQ=255)

                                                                                                                         tGCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCct               >  2:594298/1‑151 (MQ=255)

                                                                                                                          gCCAACCTCGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCct               <  1:522365/150‑1 (MQ=255)

                                                                                                                                  cGAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTtgt       <  1:281193/150‑1 (MQ=255)

                                                                                                                                   gAATTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTgg     <  2:630249/151‑1 (MQ=255)

                                                                                                                                     aTTTAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGt    >  1:281214/1‑150 (MQ=255)

                                                                                                                                       ttAATGCTGAGGGCACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAg  <  1:379225/150‑1 (MQ=255)

ACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAG  >  NC_000913/2441666‑2441950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A