Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I2 R1 173 150.8 4954114 96.5% 4780720 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,534,187 A→G E41G (GAA→GGA)  ptsI → PEP‑protein phosphotransferase of PTS system (enzyme I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,534,1870AG99.4% 588.8 / NA 155E41G (GAA→GGA) ptsIPEP‑protein phosphotransferase of PTS system (enzyme I)
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (73/81);  total (73/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 155 total aligned reads displayed.

AAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGAAAT  >  NC_000913/2534039‑2534331
                                                                                                                                                     |                                                                                                                                                
aaaaTCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGAc                                                                                                                                                <  1:1788069/151‑1 (MQ=255)
 aaaTCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACg                                                                                                                                               <  4:1345976/151‑1 (MQ=255)
 aaaTCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACg                                                                                                                                               >  2:490534/1‑151 (MQ=255)
   aTCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGtt                                                                                                                                             <  1:727067/151‑1 (MQ=255)
     cAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGtttt                                                                                                                                           <  1:1417517/151‑1 (MQ=255)
      aGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTc                                                                                                                                          >  4:1250438/1‑151 (MQ=255)
         cacaAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGa                                                                                                                                       <  1:480799/151‑1 (MQ=255)
          acaAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGTCCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTg                                                                                                                                        >  1:1378491/1‑149 (MQ=255)
           caAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGc                                                                                                                                     >  1:319713/1‑151 (MQ=255)
                   gTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGc                                                                                                                             <  2:64768/151‑1 (MQ=255)
                   gTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGc                                                                                                                             >  1:54881/1‑151 (MQ=255)
                    tAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGcc                                                                                                                            >  4:916036/1‑151 (MQ=255)
                    tAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGcc                                                                                                                            >  4:424033/1‑151 (MQ=255)
                     aGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGcc                                                                                                                            <  4:1500316/150‑1 (MQ=255)
                     aGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGcc                                                                                                                            >  1:546854/1‑150 (MQ=255)
                     aGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTGTCTGAGCGGTCGTGCCa                                                                                                                           <  1:1637572/151‑1 (MQ=255)
                           aTGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCAt                                                                                                                     >  4:598851/1‑151 (MQ=255)
                             gATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATc                                                                                                                    <  1:713498/150‑1 (MQ=255)
                             gATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATc                                                                                                                    >  1:334619/1‑150 (MQ=255)
                             gATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCa                                                                                                                   <  4:209280/151‑1 (MQ=255)
                              aTTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATc                                                                                                                    >  3:226482/1‑149 (MQ=255)
                                ttCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGcc                                                                                                                >  4:301959/1‑151 (MQ=255)
                                ttCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGcc                                                                                                                >  1:981621/1‑151 (MQ=255)
                                  cAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGccc                                                                                                               >  1:202759/1‑150 (MQ=255)
                                  cAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGGTGATCAGGAAGTTGGACGTTTTCTGAGCGGGCGTGCCAAGGCATCAGccc                                                                                                               >  4:38771/1‑150 (MQ=255)
                                   aGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGACAAGGCATAAGc                                                                                                                 >  3:34908/1‑147 (MQ=255)
                                    ggCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGc                                                                                                            <  4:1581558/151‑1 (MQ=255)
                                     gCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCt                                                                                                           >  2:150513/1‑151 (MQ=255)
                                      cATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTg                                                                                                          <  1:741293/151‑1 (MQ=255)
                                      cATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTg                                                                                                          >  4:1393105/1‑151 (MQ=255)
                                       aTTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTgg                                                                                                         >  4:1646170/1‑151 (MQ=255)
                                         tttAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGa                                                                                                        >  4:709628/1‑150 (MQ=255)
                                          ttAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGaa                                                                                                       <  2:356026/150‑1 (MQ=255)
                                          ttAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGaa                                                                                                       <  1:989491/150‑1 (MQ=255)
                                          ttAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGaa                                                                                                       <  4:1190742/150‑1 (MQ=255)
                                            aGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGTAAAc                                                                                                     <  4:1474028/150‑1 (MQ=255)
                                            aGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACg                                                                                                    <  1:246163/151‑1 (MQ=255)
                                            aGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACg                                                                                                    <  1:406461/151‑1 (MQ=255)
                                              cATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGAt                                                                                                  <  3:150506/151‑1 (MQ=255)
                                                tCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATc                                                                                                 >  1:215313/1‑150 (MQ=255)
                                                  cccGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCaa                                                                                               >  1:1475466/1‑150 (MQ=255)
                                                   ccGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCaaaa                                                                                             <  1:1056136/151‑1 (MQ=255)
                                                           cGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCt                                                                                     <  4:1246052/151‑1 (MQ=255)
                                                           cGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCt                                                                                     <  4:31244/151‑1 (MQ=255)
                                                            gCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTg                                                                                    >  1:1591248/1‑151 (MQ=255)
                                                               ttCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTgg                                                                                   <  4:1530643/149‑1 (MQ=255)
                                                               ttCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGt                                                                                  <  1:1634276/150‑1 (MQ=255)
                                                                tCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                                                 <  4:141085/150‑1 (MQ=255)
                                                                     aaaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGt                                                                           >  4:133694/1‑151 (MQ=255)
                                                                     aaaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGt                                                                           >  2:97466/1‑151 (MQ=255)
                                                                      aaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGtt                                                                          <  4:1186488/151‑1 (MQ=255)
                                                                      aaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGtt                                                                          <  1:294884/151‑1 (MQ=255)
                                                                      aaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGtt                                                                          >  1:1720978/1‑151 (MQ=255)
                                                                       aGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTc                                                                         <  1:1317159/151‑1 (MQ=255)
                                                                        gCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCg                                                                        <  4:1713647/151‑1 (MQ=255)
                                                                           ctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTg                                                                     >  1:133325/1‑151 (MQ=255)
                                                                            tGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTg                                                                     >  1:488610/1‑150 (MQ=255)
                                                                             gCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGt                                                                      <  1:1698373/148‑1 (MQ=255)
                                                                             gCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTga                                                                    >  1:1004665/1‑150 (MQ=255)
                                                                             gCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTg                                                                     <  4:749279/149‑1 (MQ=255)
                                                                             gCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTg                                                                     <  4:482950/149‑1 (MQ=255)
                                                                               ttCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTga                                                                    >  4:1062989/1‑148 (MQ=255)
                                                                                tttggaaaaagaCCGAATTGTTATTTACCCGAAAAAAAATTCTGCCCACCCAGGTGGATAGGGAAGTTGACCGTTTTCGAGGCGGCGGGGCAAAGCCTTCAG‑CCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGt                                                                      <  1:414753/137‑1 (MQ=255)
                                                                                  tGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaagaa                                                                >  1:346546/1‑149 (MQ=255)
                                                                                   gAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaagaa                                                                <  2:413587/148‑1 (MQ=255)
                                                                                   gAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaaa                                                              <  3:43562/150‑1 (MQ=255)
                                                                                    aaaGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaaa                                                              >  1:1446154/1‑149 (MQ=255)
                                                                                    aaaGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaag                                                            <  3:498439/151‑1 (MQ=255)
                                                                                     aagaagACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaaga                                                           <  1:185102/151‑1 (MQ=255)
                                                                                      agaagaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaagaa                                                          <  3:392906/151‑1 (MQ=255)
                                                                                         agaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGcc                                                       >  1:1023666/1‑151 (MQ=255)
                                                                                             gAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCt                                                   <  2:246956/151‑1 (MQ=255)
                                                                                                 ttGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTg                                                >  4:195618/1‑150 (MQ=255)
                                                                                                 ttGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTg                                                >  3:356193/1‑150 (MQ=255)
                                                                                                  tGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAG‑AAAAGAAGCCATCctt                                                 >  4:1908915/1‑147 (MQ=255)
                                                                                                   gTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGa                                               >  3:516487/1‑149 (MQ=255)
                                                                                                    tCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAgg                                            >  1:1779158/1‑151 (MQ=255)
                                                                                                      aTTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGc                                          <  1:169925/151‑1 (MQ=255)
                                                                                                       ttGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGc                                          >  3:545047/1‑150 (MQ=255)
                                                                                                        tGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCa                                         <  3:64558/150‑1 (MQ=255)
                                                                                                        tGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCa                                         <  1:1054963/150‑1 (MQ=255)
                                                                                                         gACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCat                                        <  4:330061/150‑1 (MQ=255)
                                                                                                         gACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCat                                        >  1:942776/1‑150 (MQ=255)
                                                                                                           ccGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCAGCTTTGAAGGGCAtatt                                     <  3:163871/151‑1 (MQ=255)
                                                                                                            cGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta                                    <  4:60364/151‑1 (MQ=255)
                                                                                                            cGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta                                    <  4:851681/151‑1 (MQ=255)
                                                                                                            cGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta                                    <  1:717622/151‑1 (MQ=255)
                                                                                                              gAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtg                                  <  4:796250/151‑1 (MQ=255)
                                                                                                               aaaaaaaTTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAGACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc                                 >  3:236121/1‑151 (MQ=255)
                                                                                                                   aaaTTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATgctgct                             >  1:444153/1‑151 (MQ=255)
                                                                                                                          tGCCGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGa                          >  3:375181/1‑147 (MQ=255)
                                                                                                                             cGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATgag                   <  3:108377/151‑1 (MQ=255)
                                                                                                                             cGA‑CCAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATg                     >  4:1288406/1‑149 (MQ=255)
                                                                                                                                 ccAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATgagga                 <  4:491699/150‑1 (MQ=255)
                                                                                                                                 ccAGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATgagg                  >  1:250765/1‑149 (MQ=255)
                                                                                                                                   aGGTTGATCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCt              >  4:1006217/1‑151 (MQ=255)
                                                                                                                                          tCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGc         >  1:1912085/1‑149 (MQ=255)
                                                                                                                                          tCAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGc         >  2:425685/1‑149 (MQ=255)
                                                                                                                                           cAGGAAGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAg       <  2:526385/150‑1 (MQ=255)
                                                                                                                                               aaGTTGGACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGAAAt  <  2:39507/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
AAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA‑CCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTGCTCGAAGATGAGGAGCTGGAGCAGGAAAT  >  NC_000913/2534039‑2534331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A