Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,405,951	C→T	intergenic (+302/‑28)	abgR → / → smrA	putative DNA‑binding transcriptional regulator of abgABT operon/DNA endonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,405,951	0	C	T	100.0%	139.2 / NA	35	intergenic (+302/‑28)	abgR/smrA	putative DNA‑binding transcriptional regulator of abgABT operon/DNA endonuclease
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/5); total (30/5)

AAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATC  >  NC_000913/1405813‑1406095

aaaTATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAAtt                                                                                                                                      <  2:445282/151‑1 (MQ=255)

               tattaATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAAc                                                                                                                       >  2:327905/1‑151 (MQ=255)

                attaATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAAc                                                                                                                       >  2:670506/1‑150 (MQ=255)

                 ttaATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACAt                                                                                                                     >  1:448323/1‑151 (MQ=255)

                       tCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCt                                                                                                               >  1:191181/1‑151 (MQ=255)

                         gTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTg                                                                                                             >  2:138782/1‑151 (MQ=255)

                          tgaatgaaTAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTg                                                                                                             >  1:28252/1‑150 (MQ=255)

                                     tCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGc                                                                                                 >  1:168080/1‑151 (MQ=255)

                                     tCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGc                                                                                                 >  2:122666/1‑151 (MQ=255)

                                          cATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGttt                                                                                            >  2:755146/1‑151 (MQ=255)

                                          cATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGttt                                                                                            >  1:404345/1‑151 (MQ=255)

                                            tAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCt                                                                                          >  1:349822/1‑151 (MQ=255)

                                             aaGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCt                                                                                          <  1:68722/150‑1 (MQ=255)

                                                 aTTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACg                                                                                     >  1:259150/1‑151 (MQ=255)

                                                                  ggCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTcc                                                                      >  1:687148/1‑149 (MQ=255)

                                                                   gCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGc                                                                   >  1:563566/1‑151 (MQ=255)

                                                                    cAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGcc                                                                  >  2:354549/1‑151 (MQ=255)

                                                                              aaCTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGt                                                        >  2:328512/1‑151 (MQ=255)

                                                                                    ttaCTGGCAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTAcc                                                  >  1:418313/1‑151 (MQ=255)

                                                                                     taCTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCg                                                 >  1:480927/1‑151 (MQ=255)

                                                                                            aaTTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTcc                                           >  1:583254/1‑150 (MQ=255)

                                                                                             aTTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCAc                                         >  2:133631/1‑151 (MQ=255)

                                                                                              tttGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACt                                        >  1:216428/1‑151 (MQ=255)

                                                                                                tGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTg                                       >  2:77647/1‑150 (MQ=255)

                                                                                                   gTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCAt                                   >  1:398969/1‑151 (MQ=255)

                                                                                                      aTACGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATcc                                 >  1:373620/1‑150 (MQ=255)

                                                                                                         cGTTGTTGGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAAcg                             >  1:180941/1‑151 (MQ=255)

                                                                                                            tgttgGACCGACCCGGTCTGGTTATCATATTGCGCTCTTAATCGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGt                          >  2:1664/1‑151 (MQ=255)

                                                                                                                         cGGTCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTgcgc             <  1:327918/151‑1 (MQ=255)

                                                                                                                            tCTGGTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCTCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGcgccgc          >  2:300738/1‑151 (MQ=255)

                                                                                                                               ggTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCTCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGc       <  1:300751/151‑1 (MQ=255)

                                                                                                                               ggTTATCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGc       >  2:455627/1‑151 (MQ=255)

                                                                                                                                   aTCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTAt   >  1:145571/1‑151 (MQ=255)

                                                                                                                                   aTCATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTAt   >  2:575242/1‑151 (MQ=255)

                                                                                                                                     cATATTGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATc  <  1:670546/150‑1 (MQ=255)

AAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATC  >  NC_000913/1405813‑1406095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A