Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I1 R1 163 55.0 1534755 98.6% 1513268 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,808,794 T→C H147R (CAC→CGC)  yniB ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,808,7940TC100.0% 167.0 / NA 45H147R (CAC→CGC) yniBputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/21);  total (24/21)

AGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCA  >  NC_000913/1808647‑1808938
                                                                                                                                                   |                                                                                                                                                
agcagcATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGgcgc                                                                                                                                                <  1:694772/150‑1 (MQ=255)
        gATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAacaaca                                                                                                                                       <  1:414401/151‑1 (MQ=255)
           atGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAAt                                                                                                                                     <  1:319439/150‑1 (MQ=255)
           atGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAAt                                                                                                                                     <  1:332891/150‑1 (MQ=255)
           atGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATa                                                                                                                                    <  2:636684/151‑1 (MQ=255)
              ggTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACgg                                                                                                                                 >  2:188860/1‑151 (MQ=255)
                ttGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACggg                                                                                                                                >  2:466793/1‑150 (MQ=255)
                  gAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGAGGAACAACAATACGGGACt                                                                                                                             <  2:686582/151‑1 (MQ=255)
                   aGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTc                                                                                                                            >  2:765163/1‑151 (MQ=255)
                      aTAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAAt                                                                                                                         <  1:653138/151‑1 (MQ=255)
                              cGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCAc                                                                                                                 >  2:224226/1‑151 (MQ=255)
                                 tCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCAcgcg                                                                                                              >  1:429866/1‑151 (MQ=255)
                                  ccGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGt                                                                                                             <  2:32330/151‑1 (MQ=255)
                                    gTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTa                                                                                                           <  2:260885/151‑1 (MQ=255)
                                          ccTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCct                                                                                                     <  2:290310/151‑1 (MQ=255)
                                                  ttAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCt                                                                                             >  1:118561/1‑151 (MQ=255)
                                                    aaataaaCCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCtt                                                                                            <  2:29184/150‑1 (MQ=255)
                                                             cAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTc                                                                                  >  1:201692/1‑151 (MQ=255)
                                                                gCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTcc                                                                                 >  1:598824/1‑149 (MQ=255)
                                                                  agagAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGa                                                                              >  1:248175/1‑150 (MQ=255)
                                                                   gagaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAAt                                                                            <  1:145658/151‑1 (MQ=255)
                                                                       aaaGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGt                                                                        <  1:612843/151‑1 (MQ=255)
                                                                       aaaGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGt                                                                        <  1:604039/151‑1 (MQ=255)
                                                                          gAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGTCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGa                                                                     <  2:274522/151‑1 (MQ=255)
                                                                           aaCACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGa                                                                     <  2:364611/150‑1 (MQ=255)
                                                                              acaTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCtt                                                                 >  1:463810/1‑151 (MQ=255)
                                                                               caTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGGGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc                                                                >  2:80440/1‑151 (MQ=255)
                                                                                   aCCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAac                                                             >  1:43086/1‑150 (MQ=255)
                                                                                           caATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCg                                                    >  2:267753/1‑151 (MQ=255)
                                                                                            aaTACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGc                                                   >  1:529451/1‑151 (MQ=255)
                                                                                               aCAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCa                                                  >  1:193531/1‑149 (MQ=255)
                                                                                                 aaaTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaa                                              <  2:339066/151‑1 (MQ=255)
                                                                                                     aaCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcg                                           <  2:502873/150‑1 (MQ=255)
                                                                                                        cGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGcc                                       >  1:650595/1‑151 (MQ=255)
                                                                                                            aGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTgg                                                                                         >  1:706846/1‑97 (MQ=255)
                                                                                                            aGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTgg                                                                                         <  2:706803/97‑1 (MQ=255)
                                                                                                            aGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGc                                   >  2:122705/1‑151 (MQ=255)
                                                                                                                  taCAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTc                              >  1:593459/1‑150 (MQ=255)
                                                                                                                  taCAGTGAGAAAAACTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTc                              >  1:124736/1‑150 (MQ=255)
                                                                                                                            aaaaCTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCAcc                   >  2:755998/1‑151 (MQ=255)
                                                                                                                                cTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGa                >  1:499674/1‑150 (MQ=255)
                                                                                                                                cTGCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGa                >  1:388880/1‑150 (MQ=255)
                                                                                                                                  gCAGGAAAATAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAAt              <  2:136536/150‑1 (MQ=255)
                                                                                                                                       aaaaTACTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGc         >  2:132990/1‑150 (MQ=255)
                                                                                                                                         aaTAGTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAAc      <  2:248165/151‑1 (MQ=255)
                                                                                                                                             gTATGGCGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCa  <  1:648371/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCA  >  NC_000913/1808647‑1808938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A