Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,907,238	T→C	V66V (GTA→GTG)	cspC ←	stress protein, member of the CspA‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,907,238	0	T	C	100.0%	191.6 / NA	50	V66V (GTA→GTG)	cspC	stress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/34); total (16/34)

GTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGT  >  NC_000913/1907092‑1907375

gtgtAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGt                                                                                                                                       <  1:345260/151‑1 (MQ=255)

     aCGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACa                                                                                                                                  <  2:349516/151‑1 (MQ=255)

      cgcgAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAg                                                                                                                                 <  2:223523/151‑1 (MQ=255)

         gAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTg                                                                                                                              <  2:533166/151‑1 (MQ=255)

                  ggcggGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCt                                                                                                                      <  2:119004/150‑1 (MQ=255)

                    cggGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTc                                                                                                                   <  1:37358/151‑1 (MQ=255)

                    cggGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTc                                                                                                                   <  2:17856/151‑1 (MQ=255)

                      ggCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTg                                                                                                                 <  1:631481/151‑1 (MQ=255)

                       gCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTgg                                                                                                                >  1:83691/1‑151 (MQ=255)

                         aaaTATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGcc                                                                                                              <  1:179716/151‑1 (MQ=255)

                           atatCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGt                                                                                                            <  1:429267/151‑1 (MQ=255)

                               ccGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTg                                                                                                        >  1:352748/1‑151 (MQ=255)

                               ccGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTg                                                                                                        <  2:127334/151‑1 (MQ=255)

                                cGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTg                                                                                                        >  1:87803/1‑150 (MQ=255)

                                 gAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGaa                                                                                                      <  2:48069/151‑1 (MQ=255)

                                    cGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAAttt                                                                                                   <  1:147172/151‑1 (MQ=255)

                                    cGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAAttt                                                                                                   <  1:702007/151‑1 (MQ=255)

                                          gAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACt                                                                                             >  1:568306/1‑151 (MQ=255)

                                              gAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAAc                                                                                         <  2:661717/151‑1 (MQ=255)

                                                      aGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGAc                                                                                 <  2:52845/151‑1 (MQ=255)

                                                           aGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCa                                                                            <  1:215802/151‑1 (MQ=255)

                                                                aaaGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCag                                                                       <  2:493768/151‑1 (MQ=255)

                                                                aaaGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCag                                                                       <  2:141513/151‑1 (MQ=255)

                                                                 aaGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGcc                                                                         >  1:518907/1‑148 (MQ=255)

                                                                   gCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGCTTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCaga                                                                      >  2:344852/1‑149 (MQ=255)

                                                                     cTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCag                                                                       >  2:544730/1‑146 (MQ=255)

                                                                     cTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCag                                                                       <  1:544758/146‑1 (MQ=255)

                                                                         cATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                              <  2:94785/151‑1 (MQ=255)

                                                                            tCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                            <  1:389097/150‑1 (MQ=255)

                                                                              gacgaGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt                                                         <  2:504525/151‑1 (MQ=255)

                                                                               acgaGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAtt                                                        <  1:904/151‑1 (MQ=255)

                                                                                    gCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCt                                                   >  1:246297/1‑151 (MQ=255)

                                                                                     cTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                                  >  2:622186/1‑151 (MQ=255)

                                                                                      tttATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                                  >  2:712263/1‑150 (MQ=255)

                                                                                         ataGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATa                                              <  1:544231/151‑1 (MQ=255)

                                                                                          taGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATa                                              <  2:732407/150‑1 (MQ=255)

                                                                                            gCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg                                           <  2:518883/151‑1 (MQ=255)

                                                                                               agaTTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                        <  1:416651/151‑1 (MQ=255)

                                                                                                    gAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                   >  2:51242/1‑151 (MQ=255)

                                                                                                      aGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtgt                                  >  2:671963/1‑150 (MQ=255)

                                                                                                      aGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                 <  1:336369/151‑1 (MQ=255)

                                                                                                        cGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                               <  1:104139/151‑1 (MQ=255)

                                                                                                           aTTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAac                            >  2:344153/1‑151 (MQ=255)

                                                                                                             tCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca                           <  1:600956/150‑1 (MQ=255)

                                                                                                                   ttCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCttt                    >  1:703247/1‑151 (MQ=255)

                                                                                                                   ttCAGATCAGTGGATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt                     >  1:600566/1‑150 (MQ=255)

                                                                                                                              ggATTCGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCTATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg         <  2:757590/151‑1 (MQ=255)

                                                                                                                                   cGATCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCTGa    <  2:136873/151‑1 (MQ=255)

                                                                                                                                     aTCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt  <  2:87802/151‑1 (MQ=255)

                                                                                                                                      tCAGATAGCTGTCACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt  >  2:577612/1‑150 (MQ=255)

GTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGT  >  NC_000913/1907092‑1907375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A