Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,005	T→C	H177R (CAC→CGC)	proQ ←	RNA chaperone, putative ProP translation regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,005	0	T	C	100.0%	151.8 / NA	43	H177R (CAC→CGC)	proQ	RNA chaperone, putative ProP translation regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/24); total (19/24)

CGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCAC  >  NC_000913/1914858‑1915149

cgcACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCt                                                                                                                                               <  1:335101/151‑1 (MQ=255)

   acaATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGTTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGtt                                                                                                                                            <  2:55075/151‑1 (MQ=255)

        cAAAGACATACCCGAATGCATCTGGACGCGGACGCCGTCTTTGGTGATTTCTAGTACGGTGGCATACAGCGCGTTTTGACCCGCTTGCACCTTCAGGGCTTGGCCGACAGTGAGAGCTGAAATGTCAGAAACCGGGTTGCGCTGtgctgg                                                                                                                                        <  2:113171/150‑3 (MQ=255)

         aaaGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTcgc                                                                                                                                      >  2:447897/1‑151 (MQ=255)

                 aCCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGc                                                                                                                               <  2:106610/150‑1 (MQ=255)

                  cccGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCtt                                                                                                                             <  2:65108/151‑1 (MQ=255)

                  cccGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCtt                                                                                                                             <  2:461717/151‑1 (MQ=255)

                  cccGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCt                                                                                                                              <  2:436053/150‑1 (MQ=255)

                   ccGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCttt                                                                                                                            <  1:590521/151‑1 (MQ=255)

                    cGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCtttt                                                                                                                           >  1:20802/1‑151 (MQ=255)

                     gAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTa                                                                                                                          <  2:562589/151‑1 (MQ=255)

                       aTTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACt                                                                                                                        <  1:581898/151‑1 (MQ=255)

                               ggacgcggacgcCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTgg                                                                                                                >  2:233587/1‑151 (MQ=255)

                                 acgcggacgcCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGgcg                                                                                                              >  2:98458/1‑151 (MQ=255)

                                  cgcggacgcCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGgcgc                                                                                                             <  1:100905/151‑1 (MQ=255)

                                         gcCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTctct                                                                                                      <  1:303073/151‑1 (MQ=255)

                                           cGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTAc                                                                                                    <  2:670351/151‑1 (MQ=255)

                                            gTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTAcc                                                                                                   <  2:397606/151‑1 (MQ=255)

                                            gTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGCTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTAcc                                                                                                   <  1:657987/151‑1 (MQ=255)

                                                 tGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCtt                                                                                              >  2:361586/1‑151 (MQ=255)

                                                    tGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTg                                                                                           >  2:203849/1‑151 (MQ=255)

                                                     gATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTg                                                                                           <  2:310709/150‑1 (MQ=255)

                                                        ttCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTgcgc                                                                                        >  2:446696/1‑150 (MQ=255)

                                                                     gCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGtt                                                                          <  2:130728/151‑1 (MQ=255)

                                                                           atcGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAgcgc                                                                    <  2:586559/151‑1 (MQ=255)

                                                                              gcgTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCctt                                                                 >  1:663823/1‑151 (MQ=255)

                                                                              gcgTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCctt                                                                 >  2:348162/1‑151 (MQ=255)

                                                                                    tGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTg                                                            <  2:461610/150‑1 (MQ=255)

                                                                                                    tCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTc                                             <  1:479675/149‑1 (MQ=255)

                                                                                                     cAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCgg                                           <  2:255025/150‑1 (MQ=255)

                                                                                                     cAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCgg                                           >  2:677876/1‑150 (MQ=255)

                                                                                                            tGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCt                                   >  2:251006/1‑151 (MQ=255)

                                                                                                                cGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCg                               >  2:637160/1‑151 (MQ=255)

                                                                                                                 gACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGt                              >  2:532173/1‑151 (MQ=255)

                                                                                                                    aGTCAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcg                           >  1:297351/1‑151 (MQ=255)

                                                                                                                       cAGAGCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcg                         <  2:663083/150‑1 (MQ=255)

                                                                                                                        agagCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggc                       <  1:520580/151‑1 (MQ=255)

                                                                                                                        agagCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcgg                        >  2:360674/1‑150 (MQ=255)

                                                                                                                          agCTGAAATGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggc                       >  2:497835/1‑149 (MQ=255)

                                                                                                                               aaaTGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGc                <  1:528597/151‑1 (MQ=255)

                                                                                                                                 aTGTCAGAAACCGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGt              >  2:193542/1‑151 (MQ=255)

                                                                                                                                            cGGGGTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTtctc    >  2:667895/1‑150 (MQ=255)

                                                                                                                                             ggggTGCGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCAc  >  2:305239/1‑151 (MQ=255)

CGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCAC  >  NC_000913/1914858‑1915149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A