Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,178,383	C→T	R61H (CGT→CAT)	fbaB ←	fructose‑bisphosphate aldolase class I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,178,383	0	C	T	100.0%	176.0 / NA	44	R61H (CGT→CAT)	fbaB	fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/29); total (15/29)

GCCACACAGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCG  >  NC_000913/2178236‑2178529

gCCACACAGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGcc                                                                                                                                                 <  1:453449/151‑1 (MQ=255)

 ccACACAGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGccc                                                                                                                                                >  2:598219/1‑151 (MQ=255)

  cacacaGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCg                                                                                                                                               <  1:246459/151‑1 (MQ=255)

      caGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGgtgt                                                                                                                                           >  1:290361/1‑151 (MQ=255)

       aGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGtgtt                                                                                                                                          <  1:620612/151‑1 (MQ=255)

         ttACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGtgttgt                                                                                                                                        >  1:413345/1‑151 (MQ=255)

           aCAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTa                                                                                                                                       <  2:136361/150‑1 (MQ=255)

                ccGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGt                                                                                                                                 >  1:219853/1‑151 (MQ=255)

                 cGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTc                                                                                                                                <  2:230305/151‑1 (MQ=255)

                     tCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTg                                                                                                                              <  1:231836/149‑1 (MQ=255)

                      cGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCat                                                                                                                           >  1:250526/1‑151 (MQ=255)

                        aTCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCatat                                                                                                                         <  2:690449/151‑1 (MQ=255)

                          cGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATAtt                                                                                                                        <  2:23835/150‑1 (MQ=255)

                           gCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTAc                                                                                                                      <  2:576179/151‑1 (MQ=255)

                              aGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTa                                                                                                                   <  1:378982/151‑1 (MQ=255)

                               gTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTaa                                                                                                                  <  1:257508/151‑1 (MQ=255)

                                 tCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAac                                                                                                                 >  1:289323/1‑150 (MQ=255)

                                  caacaaTGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAacac                                                                                                               >  2:332173/1‑151 (MQ=255)

                                    acaaTGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCg                                                                                                             <  1:542324/151‑1 (MQ=255)

                                      aaTGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCt                                                                                                           >  2:221123/1‑151 (MQ=255)

                                       aTGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTg                                                                                                          <  2:289311/151‑1 (MQ=255)

                                             ttCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTggcggg                                                                                                     <  2:75083/150‑1 (MQ=255)

                                                 ggTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGatt                                                                                                <  2:744383/151‑1 (MQ=255)

                                                    cAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGattatt                                                                                             >  1:249734/1‑151 (MQ=255)

                                                       aGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTc                                                                                          <  2:713541/151‑1 (MQ=255)

                                                                ggTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTAc                                                                                 >  1:33086/1‑151 (MQ=255)

                                                                 gTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAGAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTAcg                                                                                >  1:8792/1‑151 (MQ=255)

                                                                  ttAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGattatt                                                                                             >  2:407525/1‑137 (MQ=255)

                                                                  ttAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGattatt                                                                                             <  1:407546/137‑1 (MQ=255)

                                                                    agcagcAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTAcgcg                                                                              <  1:273736/150‑1 (MQ=255)

                                                                       agcaAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCt                                                                          <  1:533833/151‑1 (MQ=255)

                                                                                     cGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTcc                                                            <  2:351735/151‑1 (MQ=255)

                                                                                          gagTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGga                                                       <  2:344609/151‑1 (MQ=255)

                                                                                            gTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGgaga                                                     <  2:758682/151‑1 (MQ=255)

                                                                                            gTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGgaga                                                     <  1:344335/151‑1 (MQ=255)

                                                                                                  aaCGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGc                                               <  1:517069/151‑1 (MQ=255)

                                                                                                         tGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTc                                          >  2:99512/1‑149 (MQ=255)

                                                                                                         tGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTc                                          >  1:57305/1‑149 (MQ=255)

                                                                                                          ggTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCa                                         <  1:226850/149‑1 (MQ=255)

                                                                                                                        aTAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACGCCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATAc                         <  2:76333/151‑1 (MQ=255)

                                                                                                                        aTAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATAc                         <  1:99513/151‑1 (MQ=255)

                                                                                                                            aaaGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACg                     >  2:6461/1‑151 (MQ=255)

                                                                                                                                    ccTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTa             <  2:531511/151‑1 (MQ=255)

                                                                                                                                               cagATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCg  <  1:441690/151‑1 (MQ=255)

GCCACACAGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCG  >  NC_000913/2178236‑2178529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A