Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I2 R1	150	68.2	2132745	98.7%	2105019	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,178,383	C→T	R61H (CGT→CAT)	fbaB ←	fructose‑bisphosphate aldolase class I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,178,383	0	C	T	100.0%	203.2 / NA	50	R61H (CGT→CAT)	fbaB	fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/24); total (26/24)

CGCCACACAGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGG  >  NC_000913/2178235‑2178530

cGCCACACAGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGc                                                                                                                                                   <  2:335054/151‑1 (MQ=255)

       caGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGgtgt                                                                                                                                            <  1:43050/151‑1 (MQ=255)

         gTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGtgttg                                                                                                                                          >  1:1012932/1‑151 (MQ=255)

         gTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGtgttg                                                                                                                                          <  2:1012891/151‑1 (MQ=255)

         gTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGtgttg                                                                                                                                          >  1:429889/1‑151 (MQ=255)

            aCAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTAc                                                                                                                                       >  1:671172/1‑151 (MQ=255)

            aCAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTAc                                                                                                                                       >  1:963909/1‑151 (MQ=255)

              aGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACa                                                                                                                                      >  2:1027560/1‑150 (MQ=255)

              aGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACa                                                                                                                                      >  1:629315/1‑150 (MQ=255)

                cccGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACg                                                                                                                                   >  1:681632/1‑151 (MQ=255)

                cccGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACg                                                                                                                                   <  1:586829/151‑1 (MQ=255)

                     ttCGATCGCCAGTTCAACAATGTTTTTCGGGTCAGAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGc                                                                                                                              >  1:53201/1‑151 (MQ=255)

                        gATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCata                                                                                                                           <  2:391516/151‑1 (MQ=255)

                            gCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTAc                                                                                                                       <  1:391482/151‑1 (MQ=255)

                              cAGTTCAACAATGTTTTTCGGGTAAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGt                                                                                                                     >  2:819678/1‑151 (MQ=255)

                                gTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTa                                                                                                                    >  1:169999/1‑150 (MQ=255)

                                 ttCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCACATCAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCAACAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAac                                                                                                                  >  1:193255/1‑151 (MQ=255)

                                  tCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAac                                                                                                                  >  2:749465/1‑150 (MQ=255)

                                  tCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAac                                                                                                                  >  2:897450/1‑150 (MQ=255)

                                   caacaaTGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAaca                                                                                                                 <  2:80516/150‑1 (MQ=255)

                                        aTGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTg                                                                                                           <  2:791881/151‑1 (MQ=255)

                                        aTGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTg                                                                                                           <  1:786867/151‑1 (MQ=255)

                                              ttCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTggcgggc                                                                                                     >  1:660971/1‑151 (MQ=255)

                                               tCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTggcgggcg                                                                                                    >  2:764079/1‑151 (MQ=255)

                                                cGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTggcgggcg                                                                                                    >  1:33864/1‑150 (MQ=255)

                                                  ggTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAGCGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGatt                                                                                                 <  1:434345/151‑1 (MQ=255)

                                                      aaaGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTg                                                                                             <  1:973717/151‑1 (MQ=255)

                                                            gagCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATc                                                                                       >  1:499207/1‑151 (MQ=255)

                                                            gagCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATc                                                                                       <  2:345106/151‑1 (MQ=255)

                                                             agCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCa                                                                                      <  1:203676/151‑1 (MQ=255)

                                                                        agcaAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCt                                                                           <  2:161983/151‑1 (MQ=255)

                                                                            aaTGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGt                                                                       >  1:666342/1‑151 (MQ=255)

                                                                             aTGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTACCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTa                                                                      <  1:39034/151‑1 (MQ=255)

                                                                               gAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGt                                                                    <  1:969067/151‑1 (MQ=255)

                                                                               gAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGt                                                                    <  1:316954/151‑1 (MQ=255)

                                                                                     ccGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATg                                                                <  2:595631/149‑1 (MQ=255)

                                                                                      cGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTcc                                                             >  1:49893/1‑151 (MQ=255)

                                                                                         cAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCggg                                                          >  1:136111/1‑151 (MQ=255)

                                                                                         cAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCggg                                                          >  1:990285/1‑151 (MQ=255)

                                                                                              tGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCgggg                                                         >  2:426551/1‑147 (MQ=255)

                                                                                               gCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGAt                                                     <  1:638753/150‑1 (MQ=255)

                                                                                                   aaCGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGc                                                <  1:747610/151‑1 (MQ=255)

                                                                                                      gCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTgg                                             >  2:357781/1‑151 (MQ=255)

                                                                                                       cccTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGt                                            <  2:295708/151‑1 (MQ=255)

                                                                                                               aaCCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAgg                                     >  2:656524/1‑150 (MQ=255)

                                                                                                                 ccGGCAGAATAGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAAt                                  >  2:270597/1‑151 (MQ=255)

                                                                                                                           aGAAAGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACaa                        <  2:448002/151‑1 (MQ=255)

                                                                                                                             aaaGATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACg                      <  2:1044645/151‑1 (MQ=255)

                                                                                                                                gATATCCTGTGCCAGCCAGATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTg                   <  1:863906/151‑1 (MQ=255)

                                                                                                                                                 agATGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCgg  >  1:475583/1‑151 (MQ=255)

CGCCACACAGTTACAGCCCGCTTCGATCGCCAGTTCAACAATGTTTTTCGGGTCAAAGTAGAGCGGGTTAGCAGCAAATGAAGCTCCGGCAGAGTGCTCAACGCCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGG  >  NC_000913/2178235‑2178530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A