Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,438,860	A→G	D304G (GAC→GGC)	flk →	putative flagella assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,438,860	0	A	G	100.0%	190.7 / NA	51	D304G (GAC→GGC)	flk	putative flagella assembly protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/24); total (27/24)

CCAGCAAAAATATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAA  >  NC_000913/2438714‑2439006

ccAGCAAAAATATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGTGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACg                                                                                                                                                <  2:450596/151‑1 (MQ=255)

  aGCAAAAATATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGtt                                                                                                                                              <  1:449918/151‑1 (MQ=255)

    cAAAAATATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGc                                                                                                                                            <  2:467556/151‑1 (MQ=255)

      aaaaTATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTTATCCTTTTTCACCGATGATGGGCACGTTTCa                                                                                                                                           >  2:243815/1‑150 (MQ=255)

        aaTATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCaaa                                                                                                                                         >  1:672075/1‑150 (MQ=255)

         atatGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAat                                                                                                                                       <  1:751261/151‑1 (MQ=255)

                 tttCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTTCACCGATGATGGGCACGTTGCAAAATATGGCaa                                                                                                                                >  2:160719/1‑150 (MQ=255)

                  ttCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAAGGATTGATACGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAAc                                                                                                                               >  2:220424/1‑150 (MQ=255)

                    cTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGAAGGGCACGTTGCAAAAAATGGCAAcgc                                                                                                                             >  2:687305/1‑150 (MQ=255)

                      gcgcAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTc                                                                                                                          <  1:173852/151‑1 (MQ=255)

                                cGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCtgt                                                                                                                 <  2:714956/150‑1 (MQ=255)

                                  cTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGGTTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTgg                                                                                                               >  2:599293/1‑150 (MQ=255)

                                      cttctGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGGTTGATCCGCGTGGTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACt                                                                                                          >  2:190941/1‑151 (MQ=255)

                                        tctGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTg                                                                                                         <  2:693203/150‑1 (MQ=255)

                                        tctGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGt                                                                                                        >  1:623813/1‑151 (MQ=255)

                                              cAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcg                                                                                                  <  1:374696/151‑1 (MQ=255)

                                               aGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGGTTGATCCGCGTGTTATGCAACCTCTGCTTAATCCTTTTCCACCGATGAAGGGCACGTTGCAAAATATGGCAAAGCGTCCCGCGCCGTGGATACTGTTAGTc                                                                                                   >  2:592291/1‑149 (MQ=255)

                                               aGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATTCAACCGCCGCTTAAACCTTTTCCACCGAAGATGGGCACGATGCAAAAAATGGCCACACGGCCCACCCCGTGGATAccttttgt                                                                                                    >  2:76682/1‑141 (MQ=255)

                                                ggCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTcgcg                                                                                                <  2:612882/151‑1 (MQ=255)

                                                   cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAtt                                                                                             >  1:178929/1‑151 (MQ=255)

                                                   cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAtt                                                                                             >  1:225897/1‑151 (MQ=255)

                                                   cGAGGATATTCTTAATCAGCTTTATCAACGGCGGGGTAAAGGGATTGAACCGCGTGTTATGCAACCCCTGCTTAAACCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCCACCCGTCCCGCGCTGTGGGTACTGTTAGTCGCGAtt                                                                                             >  1:131968/1‑151 (MQ=255)

                                                    gAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAAGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGAtt                                                                                             >  1:439285/1‑150 (MQ=255)

                                                     aGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTAt                                                                                           <  1:107250/151‑1 (MQ=255)

                                                      ggATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTTCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGGGATTAt                                                                                           >  2:559549/1‑150 (MQ=255)

                                                       gATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATc                                                                                          >  1:43529/1‑150 (MQ=255)

                                                           ttCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCCAAATATGGCAACGCGTCCCCCGCTGTGGATACTGTTAGTCGCGATTATCCTGAt                                                                                     >  1:586502/1‑151 (MQ=255)

                                                               tAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATg                                                                                    <  2:704865/148‑1 (MQ=255)

                                                                aaTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTg                                                                                 <  2:614039/150‑1 (MQ=255)

                                                                 aTCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGt                                                                               <  2:542808/151‑1 (MQ=255)

                                                                      cTTTATCAACGGCGGGTTAAAGGGATTGATTCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctgg                                                                           >  1:458249/1‑150 (MQ=255)

                                                                        ttATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTctggctg                                                                        >  2:372457/1‑151 (MQ=255)

                                                                         tATCAACGGCGGGGTAAAGGGATTGATCCGGGTGGTATGCAAGCGCTTCTTAATCCTTTTCCACCGATTAGGGGCAAGTTGCAAAATATGGCAACGCGGCCCGCGGTTTGGATACTTGTTGGCGCGAATATCCTGATGCTGGTcttg                                                                           >  1:233711/1‑145 (MQ=255)

                                                                            cAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTc                                                                    <  1:323058/151‑1 (MQ=255)

                                                                                     gTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAAcccc                                                           <  1:639664/151‑1 (MQ=255)

                                                                                       tAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCcg                                                          >  2:215280/1‑150 (MQ=255)

                                                                                            ggATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGaaa                                                    <  2:220660/151‑1 (MQ=255)

                                                                                                  aTCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                              <  2:485363/151‑1 (MQ=255)

                                                                                                  aTCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAg                                              <  1:483053/151‑1 (MQ=255)

                                                                                                    ccGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGt                                             <  1:635434/150‑1 (MQ=255)

                                                                                                            tATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGCTCGTTAACCCCGACGAAATGACAGTATCGTGAc                                     >  1:550005/1‑150 (MQ=255)

                                                                                                             aTGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACa                                    <  2:581421/150‑1 (MQ=255)

                                                                                                                    cGCTGCTTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAcc                            >  1:710724/1‑151 (MQ=255)

                                                                                                                         ctTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGc                        >  2:509045/1‑150 (MQ=255)

                                                                                                                         ctTAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGc                        >  1:728914/1‑150 (MQ=255)

                                                                                                                          ttAATCCTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCAc                      >  2:206417/1‑151 (MQ=255)

                                                                                                                               ccTTTTCCACCGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCa                  <  2:703312/150‑1 (MQ=255)

                                                                                                                                         cGATGATGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGcccc       <  1:644330/151‑1 (MQ=255)

                                                                                                                                          gatgatGGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCg      >  2:419068/1‑151 (MQ=255)

                                                                                                                                            tgatgGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCgg     <  2:638093/150‑1 (MQ=255)

                                                                                                                                              atgGGCACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCaa  <  1:575511/151‑1 (MQ=255)

CCAGCAAAAATATGGTCTTTCTGCGCAATCATCGCTTTCTTCTGCCCAGGCCGAGGATATTCTTAATCAGCTTTATCAACGGCGGGTTAAAGGGATTGATCCGCGTGTTATGCAACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAGATCGCCCCGGCAA  >  NC_000913/2438714‑2439006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A