Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,448,157	T→C	N95S (AAC→AGC)	prmB ←	N5‑glutamine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,448,157	0	T	C	98.3%	222.4 / ‑6.8	59	N95S (AAC→AGC)	prmB	N5‑glutamine methyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (22/36); total (22/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCTACCAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCT  >  NC_000913/2448010‑2448301

ccGCTACCAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTgg                                                                                                                                               <  2:145102/151‑1 (MQ=255)

     aCCAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCa                                                                                                                                            <  1:564788/149‑1 (MQ=255)

     aCCAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCa                                                                                                                                            <  2:133895/149‑1 (MQ=255)

       cAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGt                                                                                                                                         >  1:480556/1‑150 (MQ=255)

       cAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTa                                                                                                                                        <  2:729980/151‑1 (MQ=255)

        aGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTa                                                                                                                                        >  1:325456/1‑150 (MQ=255)

             acaTATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCAc                                                                                                                                  >  2:523635/1‑151 (MQ=255)

               atatCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCTCGCTTTTCTGGTCAGGTAAGCCACCg                                                                                                                                <  2:566699/151‑1 (MQ=255)

                     aaaaTATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATg                                                                                                                           <  1:752999/150‑1 (MQ=255)

                     aaaaTATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGc                                                                                                                          >  1:232303/1‑151 (MQ=255)

                     aaaaTATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGc                                                                                                                          >  2:442750/1‑151 (MQ=255)

                               gcGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTg                                                                                                                  <  2:664535/149‑1 (MQ=255)

                                cGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCTCCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGa                                                                                                                 <  1:763971/149‑1 (MQ=255)

                                cGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGAcg                                                                                                               >  1:375645/1‑151 (MQ=255)

                                   ttgcttgcTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACgcgg                                                                                                            >  2:271193/1‑151 (MQ=255)

                                     gcttgctGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACgcggc                                                                                                           <  1:560128/150‑1 (MQ=255)

                                       ttgctGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGcggcgg                                                                                                         >  1:225988/1‑150 (MQ=255)

                                               aaGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCAcgcg                                                                                                 >  2:761956/1‑150 (MQ=255)

                                                aGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGtt                                                                                               <  2:740653/151‑1 (MQ=255)

                                                    cGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTcaac                                                                                           <  2:112054/151‑1 (MQ=255)

                                                          aTTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACg                                                                                     <  1:393321/151‑1 (MQ=255)

                                                           tttATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACgg                                                                                    >  2:381234/1‑151 (MQ=255)

                                                             tATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGgt                                                                                   <  2:365135/150‑1 (MQ=255)

                                                              aTTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGgtgt                                                                                 <  2:592717/151‑1 (MQ=255)

                                                                 gttgATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGtttt                                                                              >  1:599277/1‑151 (MQ=255)

                                                                  ttgATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGtttt                                                                              <  2:603045/150‑1 (MQ=255)

                                                                   tgATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGttttt                                                                             <  2:198880/150‑1 (MQ=255)

                                                                    gATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCg                                                                           <  1:459266/151‑1 (MQ=255)

                                                                       cAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTg                                                                        <  2:61363/151‑1 (MQ=255)

                                                                         gTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGa                                                                      <  1:279505/151‑1 (MQ=255)

                                                                           tCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTgggg                                                                     <  1:672677/150‑3 (MQ=255)

                                                                            cACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGt                                                                   >  1:91269/1‑151 (MQ=255)

                                                                               cAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAg                                                                <  1:2843/151‑1 (MQ=255)

                                                                               cAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCAACGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAg                                                                <  2:646028/151‑1 (MQ=255)

                                                                                 aTCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGa                                                               >  1:353702/1‑150 (MQ=255)

                                                                                    ggCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGAcgcg                                                           <  1:673473/151‑1 (MQ=255)

                                                                                        aGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGt                                                       <  1:503679/151‑1 (MQ=255)

                                                                                            cgGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATCGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTgcgc                                                   >  1:353572/1‑151 (MQ=255)

                                                                                                aCCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCatat                                               <  2:221015/151‑1 (MQ=255)

                                                                                                  cAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCt                                             <  1:350014/151‑1 (MQ=255)

                                                                                                       cgcgTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCgg                                        <  1:523659/151‑1 (MQ=255)

                                                                                                         cgTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGATTGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGaa                                      <  2:17593/151‑1 (MQ=255)

                                                                                                             cATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATc                                  <  1:465907/151‑1 (MQ=255)

                                                                                                                  aCGTAAAATTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCg                             <  2:483767/151‑1 (MQ=255)

                                                                                                                        aaTTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAgg                        >  1:282680/1‑150 (MQ=255)

                                                                                                                        aaTTCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGt                       <  1:675448/151‑1 (MQ=255)

                                                                                                                          ttCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAgg                        >  1:205610/1‑148 (MQ=255)

                                                                                                                          ttCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGt                       >  2:433466/1‑149 (MQ=255)

                                                                                                                           tCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGTGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTag                     <  1:79589/150‑1 (MQ=255)

                                                                                                                           tCATGGCCGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCTAGCGGCAGGTa                      >  2:228756/1‑149 (MQ=255)

                                                                                                                                 ccGCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGa                >  1:268060/1‑149 (MQ=255)

                                                                                                                                   gCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGc            <  2:645699/151‑1 (MQ=255)

                                                                                                                                   gCAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGc            <  1:270168/151‑1 (MQ=255)

                                                                                                                                    cAGAACCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGc            >  1:414965/1‑150 (MQ=255)

                                                                                                                                        aCCACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAacac       >  1:407238/1‑151 (MQ=255)

                                                                                                                                         ccACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATCGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACAcc      <  2:353558/151‑1 (MQ=255)

                                                                                                                                          cACGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCa     <  1:459203/151‑1 (MQ=255)

                                                                                                                                           aCGCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACAcc      >  1:117275/1‑149 (MQ=255)

                                                                                                                                             gCTTTGCTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCt  <  1:164328/151‑1 (MQ=255)

CCGCTACCAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCT  >  NC_000913/2448010‑2448301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A