Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	702,597	(G)_5→6	coding (155/1149 nt)	nagA ←	N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	702,592	1	.	G	100.0%	131.8 / NA	37	G54R (GGT→CGT)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (19/18); total (19/18)

CGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACC‑GGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCT  >  NC_000913/702442‑702735

cGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAAcc                                                                                                                                                  <  1:287515/151‑1 (MQ=255)

    ggtggtGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACC‑gggg                                                                                                                                             <  1:676910/151‑1 (MQ=255)

             aaGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAAt                                                                                                                                     >  1:215332/1‑151 (MQ=255)

                    ggCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCg                                                                                                                              <  2:464822/151‑1 (MQ=255)

                       aGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTc                                                                                                                           >  2:97451/1‑151 (MQ=255)

                        gATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCa                                                                                                                          <  2:499303/151‑1 (MQ=255)

                          tagttagtACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGt                                                                                                                        >  1:3987/1‑151 (MQ=255)

                               agtACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACg                                                                                                                   <  1:334483/151‑1 (MQ=255)

                                gtACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACgt                                                                                                                  <  1:70989/151‑1 (MQ=255)

                                    aGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACgttgtt                                                                                                              >  1:361416/1‑151 (MQ=255)

                                      ccTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCg                                                                                                            >  2:175138/1‑151 (MQ=255)

                                           tttCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGAtctc                                                                                                       >  2:459567/1‑151 (MQ=255)

                                              ctcATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTg                                                                                                     >  2:178333/1‑150 (MQ=255)

                                               tcATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTggc                                                                                                   >  1:563152/1‑151 (MQ=255)

                                                        ttCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTcc                                                                                          >  2:640435/1‑151 (MQ=255)

                                                         tCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTcc                                                                                          <  2:74984/150‑1 (MQ=255)

                                                               tGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCg                                                                                   >  2:555958/1‑151 (MQ=255)

                                                                 aTTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCgg                                                                                  >  1:325889/1‑150 (MQ=255)

                                                                 aTTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGa                                                                                 <  1:213125/151‑1 (MQ=255)

                                                                 aTTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGa                                                                                 <  2:369957/151‑1 (MQ=255)

                                                                     ccAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAg                                                                              <  2:92941/150‑1 (MQ=255)

                                                                      cAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGAc                                                                            <  2:194683/151‑1 (MQ=255)

                                                                        gCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGc                                                                          <  2:179754/151‑1 (MQ=255)

                                                                          gTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCtt                                                                        <  2:438861/151‑1 (MQ=255)

                                                                              ccACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTa                                                                     <  2:398886/150‑1 (MQ=255)

                                                                                       ccGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCAt                                                           <  2:83019/151‑1 (MQ=255)

                                                                                                   tgtCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATaaca                                                <  1:39705/150‑1 (MQ=255)

                                                                                                       gTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACcgc                                           <  1:325905/151‑1 (MQ=255)

                                                                                                         tAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGt                                         >  2:256996/1‑151 (MQ=255)

                                                                                                            aCTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGt                                      >  1:469756/1‑151 (MQ=255)

                                                                                                                 aCGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGtcatc                                  <  2:361406/150‑1 (MQ=255)

                                                                                                                    ccgccgcAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGtcatca                                 >  1:566399/1‑148 (MQ=255)

                                                                                                                               gTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACACCCGCGTGGTCATCAAGAAATTCGTGGcc                   <  1:606776/151‑1 (MQ=255)

                                                                                                                                  tAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGt                >  2:336829/1‑151 (MQ=255)

                                                                                                                                  tAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGt                >  2:51437/1‑151 (MQ=255)

                                                                                                                                   aaCTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGt                >  1:395428/1‑150 (MQ=255)

                                                                                                                                      tGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTaa              >  1:575260/1‑149 (MQ=255)

                                                                                                                                      tGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTaa              >  1:647817/1‑149 (MQ=255)

                                                                                                                                                 aaaaCCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCt  <  2:26925/150‑1 (MQ=255)

CGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACC‑GGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCT  >  NC_000913/702442‑702735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A