Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 209 44.2 1360995 98.7% 1343302 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 997,179 C→T A112T (GCC→ACC)  ssuE ← NAD(P)H‑dependent FMN reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913997,1790CT100.0% 173.0 / NA 43A112T (GCC→ACC) ssuENAD(P)H‑dependent FMN reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/24);  total (19/24)

GGTTTGCAGATTTGGCGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTG  >  NC_000913/997036‑997326
                                                                                                                                               |                                                                                                                                                   
ggTTTGCAGATTTGGCGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTa                                                                                                                                              <  1:408602/151‑1 (MQ=255)
         aTTTGGCGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTc                                                                                                                                     <  1:321841/151‑1 (MQ=255)
             ggCGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGcc                                                                                                                                  >  1:259970/1‑150 (MQ=255)
             ggCGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCATGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCa                                                                                                                                 <  1:137979/151‑1 (MQ=255)
               cGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGc                                                                                                                               >  1:420366/1‑151 (MQ=255)
                 tGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCgg                                                                                                                             >  1:501988/1‑151 (MQ=255)
                         ggTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGcacc                                                                                                                      >  1:473152/1‑150 (MQ=255)
                             tgtgATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACttt                                                                                                                 >  2:397766/1‑151 (MQ=255)
                               tgATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGc                                                                                                               >  2:467007/1‑151 (MQ=255)
                                       aTCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCaaa                                                                                                       <  1:160218/151‑1 (MQ=255)
                                          aaTTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAgcg                                                                                                    >  2:65081/1‑151 (MQ=255)
                                              aCTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGt                                                                                                 <  2:558937/150‑1 (MQ=255)
                                               cTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGt                                                                                                 <  1:449410/149‑1 (MQ=255)
                                                 tgtgAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCt                                                                                              <  2:547785/150‑1 (MQ=255)
                                                  gtgAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTgg                                                                                            <  1:56231/151‑1 (MQ=255)
                                                            ggCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCg                                                                                  <  1:511759/151‑1 (MQ=255)
                                                               aaaCACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGc                                                                               >  1:48250/1‑151 (MQ=255)
                                                                aaCACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGc                                                                               >  1:42667/1‑150 (MQ=255)
                                                                aaCACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGc                                                                               >  1:306774/1‑150 (MQ=255)
                                                                      ccGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGttt                                                                        >  1:284707/1‑151 (MQ=255)
                                                                            aGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACg                                                                  <  2:55565/151‑1 (MQ=255)
                                                                                 ctcCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCg                                                             >  1:612267/1‑151 (MQ=255)
                                                                                      gAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGaa                                                          <  1:232430/149‑1 (MQ=255)
                                                                                      gAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAAt                                                         <  1:113590/150‑1 (MQ=255)
                                                                                      gAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATa                                                        <  1:439493/151‑1 (MQ=255)
                                                                                        gCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAgg                                                      <  1:61734/151‑1 (MQ=255)
                                                                                            tCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAggcgg                                                   <  1:356661/150‑1 (MQ=255)
                                                                                             cAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAggcggc                                                  <  2:274027/150‑1 (MQ=255)
                                                                                                    cTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATac                                           >  1:666659/1‑150 (MQ=255)
                                                                                                             ggTTTAAGGGCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGt                                   <  1:326496/149‑1 (MQ=255)
                                                                                                                    gggCATAATCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAAt                          >  1:489130/1‑151 (MQ=255)
                                                                                                                          aaTCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGccc                    <  2:44264/151‑1 (MQ=255)
                                                                                                                          aaTCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGccc                    <  2:676061/151‑1 (MQ=255)
                                                                                                                          aaTCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGccc                    >  2:343082/1‑151 (MQ=255)
                                                                                                                          aaTCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGccc                    >  1:387231/1‑151 (MQ=255)
                                                                                                                          aaTCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGccc                    <  1:262063/151‑1 (MQ=255)
                                                                                                                          aaTCGACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGcc                     <  1:497230/150‑1 (MQ=255)
                                                                                                                              gACCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATc                 >  1:99474/1‑150 (MQ=255)
                                                                                                                               aCCGCCAGCAGATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCgg               <  1:14130/151‑1 (MQ=255)
                                                                                                                                    cagcagATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGt          >  2:100878/1‑151 (MQ=255)
                                                                                                                                         gATGGGTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGc       >  2:101076/1‑149 (MQ=255)
                                                                                                                                            gggTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTg  <  2:260809/151‑1 (MQ=255)
                                                                                                                                            gggTCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTg  <  2:248812/151‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GGTTTGCAGATTTGGCGTGAACTGGGGTCTGTGATGGTAATCAATTACTTGTGAGTCATCGGCAAACACGCCGTGCAGGATCTCCTGAGCTTTCAGTGCGCTTAAAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTG  >  NC_000913/997036‑997326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A