Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,190	A→G	V333A (GTG→GCG)	puuP ←	putrescine importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,190	0	A	G	100.0%	167.7 / NA	48	V333A (GTG→GCG)	puuP	putrescine importer
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/28); total (20/28)

AACACCGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCG  >  NC_000913/1358043‑1358333

aaCACCGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCAt                                                                                                                                              <  2:630503/151‑1 (MQ=255)

   acCGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCTGCCATTTTGGGTGCGCATAGc                                                                                                                                           >  2:150345/1‑151 (MQ=255)

   acCGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGc                                                                                                                                           <  2:88620/151‑1 (MQ=255)

    ccGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGcc                                                                                                                                          <  2:555661/151‑1 (MQ=255)

     cGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGcc                                                                                                                                          >  1:464534/1‑150 (MQ=255)

      gACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAAAAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGGCCGCCATTTTGGGTGCGCATAGCCa                                                                                                                                         >  2:636338/1‑150 (MQ=255)

         aGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAaca                                                                                                                                     <  1:362407/151‑1 (MQ=255)

                 gAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTcc                                                                                                                             <  1:427195/151‑1 (MQ=255)

                        aaCGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAgca                                                                                                                      <  2:523256/151‑1 (MQ=255)

                          cGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAAcaca                                                                                                                    <  2:426351/151‑1 (MQ=255)

                               ccaGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGt                                                                                                                <  2:271056/150‑1 (MQ=255)

                                  gagCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACg                                                                                                            <  2:339732/151‑1 (MQ=255)

                                  gagCACCGAAGTTAATTAACGCTGTGGCGGCAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATAACGTTTAGTGCCGGAGTACGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTc                                                                                                               >  1:372703/1‑148 (MQ=255)

                                   agCACCGAAGTTAATTAACGCTGTGGCGGTAACCAAATCGAAGAACCGCGCCGACAACGCCACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCCCCCTTTTGGGGGCGCCTAGCCAAACACGCGCCCCGGAAACACAATGTCAAGc                                                                                                           >  2:67153/1‑151 (MQ=255)

                                      aCCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGcccc                                                                                                        >  2:340673/1‑151 (MQ=255)

                                        cGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCAt                                                                                                      <  1:326746/151‑1 (MQ=255)

                                         gAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATc                                                                                                     <  1:219792/151‑1 (MQ=255)

                                          aaGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATc                                                                                                     >  1:567835/1‑150 (MQ=255)

                                           aGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATcac                                                                                                   >  1:65799/1‑151 (MQ=255)

                                            gTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATcaca                                                                                                  <  1:658633/151‑1 (MQ=255)

                                                     cGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGa                                                                                         <  1:330950/151‑1 (MQ=255)

                                                     cGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGa                                                                                         <  2:413808/151‑1 (MQ=255)

                                                      gCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGAc                                                                                        <  1:122390/151‑1 (MQ=255)

                                                        tgtgGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGGGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATAca                                                                                              >  1:557808/1‑143 (MQ=255)

                                                              ggTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACg                                                                                >  1:427043/1‑151 (MQ=255)

                                                                       aTCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCAtgt                                                                       <  1:323876/151‑1 (MQ=255)

                                                                          gaagaaCAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGaa                                                                    <  1:518095/151‑1 (MQ=255)

                                                                             gaaCAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgcc                                                                 <  1:45350/151‑1 (MQ=255)

                                                                                    gcCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgccaggccag                                                          <  2:57938/151‑1 (MQ=255)

                                                                                      cGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgcccggc                                                             >  2:519801/1‑146 (MQ=255)

                                                                                       gACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAgg                                                       >  2:373269/1‑151 (MQ=255)

                                                                                         cAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGc                                                      >  2:349296/1‑150 (MQ=255)

                                                                                          aaCGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCt                                                     >  2:383806/1‑150 (MQ=255)

                                                                                                aCAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACgtgt                                              <  1:406446/151‑1 (MQ=255)

                                                                                                          ccATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACg                                     >  2:244452/1‑150 (MQ=255)

                                                                                                              aaTGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACgtggtg                                <  1:125796/151‑1 (MQ=255)

                                                                                                              aaTGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACgtggtg                                <  2:572491/151‑1 (MQ=255)

                                                                                                                 gACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGc                               >  1:76398/1‑149 (MQ=255)

                                                                                                                  aCGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGc                               >  1:51751/1‑148 (MQ=255)

                                                                                                                      tGAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGa                         <  1:140490/150‑1 (MQ=255)

                                                                                                                       gAGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGaaa                       <  1:68960/151‑1 (MQ=255)

                                                                                                                        aGTGCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGaaa                       >  2:649217/1‑150 (MQ=255)

                                                                                                                           gCCGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCg                   <  1:71476/151‑1 (MQ=255)

                                                                                                                             cGGAGTCCGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGAc                 <  1:370790/151‑1 (MQ=255)

                                                                                                                                    cGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGa             >  2:448542/1‑148 (MQ=255)

                                                                                                                                    cGCCATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAaga          <  2:59359/151‑1 (MQ=255)

                                                                                                                                      ccATTTTGGGTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGGGCAGAGGAAAATCGACTGGAAGAGc        >  2:231721/1‑151 (MQ=255)

                                                                                                                                             gggTGCGCATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTgccg  <  2:377328/150‑1 (MQ=255)

AACACCGACAGATTCACGAAGGTAAACGCCACCAGAGCACCGAAGTTAATTAACGCTGTGGCGGTAACTAAATCGAAGAACAGCGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCG  >  NC_000913/1358043‑1358333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A