Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,595,660	A→G	pseudogene (2328/3861 nt)	yneO ←	pseudogene, AidA homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,595,660	0	A	G	100.0%	137.8 / NA	37	pseudogene (2328/3861 nt)	yneO	pseudogene, AidA homolog
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/14); total (23/14)

GCAGGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTCAGCGTGTAATCTCCT  >  NC_000913/1595514‑1595804

gcagGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGa                                                                                                                                              <  1:218818/151‑1 (MQ=255)

        atCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACt                                                                                                                                       <  1:116405/150‑1 (MQ=255)

                  aaaaTGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGtt                                                                                                                            >  2:303447/1‑151 (MQ=255)

                  aaaaTGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGtt                                                                                                                            >  1:159296/1‑151 (MQ=255)

                  aaaaTGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGtt                                                                                                                            >  1:224221/1‑151 (MQ=255)

                    aaTGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTgg                                                                                                                          >  1:82067/1‑151 (MQ=255)

                     atgatgGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTggg                                                                                                                         >  1:591531/1‑151 (MQ=255)

                        atgGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCaa                                                                                                                      <  1:127904/151‑1 (MQ=255)

                            tACCTCCATTCATGGCCAGTCCAACAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAacac                                                                                                                   >  1:302974/1‑150 (MQ=255)

                                 ccATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCg                                                                                                              <  2:282942/150‑1 (MQ=255)

                                 ccATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCg                                                                                                              >  1:539504/1‑150 (MQ=255)

                                      cATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTc                                                                                                         >  2:24533/1‑150 (MQ=255)

                                       aTGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTc                                                                                                         >  2:344021/1‑149 (MQ=255)

                                         ggCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGt                                                                                                      <  1:661463/150‑1 (MQ=255)

                                          gCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTcc                                                                                                    <  1:492457/151‑1 (MQ=255)

                                            cAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCt                                                                                                  >  2:263392/1‑151 (MQ=255)

                                              gTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGt                                                                                                >  1:353068/1‑151 (MQ=255)

                                                    caATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCTCGGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCAt                                                                                           <  2:593542/150‑1 (MQ=255)

                                                        ggATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTa                                                                                      <  1:602034/151‑1 (MQ=255)

                                                              ctctCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGcc                                                                                 >  2:677161/1‑150 (MQ=255)

                                                                ctcCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCaa                                                                               >  2:49914/1‑150 (MQ=255)

                                                                    aaCTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATc                                                                          >  1:614385/1‑151 (MQ=255)

                                                                        tttACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGt                                                                      >  1:635111/1‑151 (MQ=255)

                                                                          tACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTc                                                                     <  1:14959/150‑1 (MQ=255)

                                                                                        tttCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTg                                                      >  1:599009/1‑151 (MQ=255)

                                                                                            aCTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACt                                                  >  2:273204/1‑151 (MQ=255)

                                                                                                    aCTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCaa                                          <  2:596375/151‑1 (MQ=255)

                                                                                                         aaCATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCAt                                     >  1:443149/1‑151 (MQ=255)

                                                                                                                  tGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTg                             >  1:198914/1‑150 (MQ=255)

                                                                                                                       aaGCGCAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAgcgc                        <  2:535272/150‑1 (MQ=255)

                                                                                                                         gcgcAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCAtt                     <  2:167079/151‑1 (MQ=255)

                                                                                                                            cAGCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGt                  >  1:153253/1‑151 (MQ=255)

                                                                                                                             cgCGGTGTTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTc                 >  2:414314/2‑151 (MQ=255)

                                                                                                                                 gtgtTGTCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTCAGCg             >  2:69202/1‑151 (MQ=255)

                                                                                                                                     tgtCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTCAGCgtgt          >  2:343021/1‑150 (MQ=255)

                                                                                                                                      gtCGCGTTCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTCAGCGTGTaa        <  2:655949/151‑1 (MQ=255)

                                                                                                                                             tCCAGGGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTCAGCGTGTAATCTCCt  <  2:377030/150‑1 (MQ=255)

GCAGGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGCGCATTGGTCAGCGTGTAATCTCCT  >  NC_000913/1595514‑1595804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A