Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,769,222	C→T	P50L (CCG→CTG)	ydiK →	UPF0118 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,769,222	0	C	T	100.0%	112.3 / NA	32	P50L (CCG→CTG)	ydiK	UPF0118 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/19); total (13/19)

ATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCC  >  NC_000913/1769074‑1769357

aTGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACctggctgg                                                                                                                                       >  1:608632/1‑151 (MQ=255)

 tGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACctggctgg                                                                                                                                       >  1:618445/1‑150 (MQ=255)

     aaaTGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGGTTGTTCAACCCTTTATTCTCGGCTTTGCCTGGGTCGGTAAGGTGGTTATCGCCACCTGGCTGGGAtt                                                                                                                                   >  1:578375/1‑150 (MQ=255)

       aTGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGtt                                                                                                                                >  2:261673/1‑151 (MQ=255)

       aTGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGtt                                                                                                                                <  1:621946/151‑1 (MQ=255)

               cAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTTTTCAACCCCTTATTTTCGGCTTTGCATTGGCCCGTCCGGTGGTTATCTCCACCTTGGTGGGATTGTTTCGgtt                                                                                                                          >  2:583309/1‑149 (MQ=255)

                 gCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCa                                                                                                                       >  1:674250/1‑150 (MQ=255)

                    cAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCaaaa                                                                                                                    >  2:505781/1‑150 (MQ=255)

                     aGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCaaa                                                                                                                     >  1:488933/1‑148 (MQ=255)

                     aGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGa                                                                                                                  <  2:578618/151‑1 (MQ=255)

                           gTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGt                                                                                                            <  1:351237/151‑1 (MQ=255)

                                 caAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTTGCTGGTCTTGTTACGTTTGCAAAAGATCATGTTTGgcc                                                                                                      >  1:604056/1‑151 (MQ=255)

                                 caAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGgcc                                                                                                      <  1:345475/151‑1 (MQ=255)

                                 caAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGgc                                                                                                       <  2:209829/150‑1 (MQ=255)

                                      tCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGccgccg                                                                                                  <  1:128535/150‑1 (MQ=255)

                                                  gCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGTTACGGTGGTTATCGCCCCCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGtt                                                                                     <  2:41387/151‑1 (MQ=255)

                                                                tcatGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGc                                                                        >  2:650323/1‑150 (MQ=255)

                                                                               gTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATgg                                                         >  1:277087/1‑150 (MQ=255)

                                                                                   gtgGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGgtg                                                       <  1:358878/148‑1 (MQ=255)

                                                                                       aTTGTTCAACCCTTTATTCTCGGTTTTGCATGGTCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTTCTTTTGCAAAAGATCATGTTTGGTCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTatc                                                 <  2:353042/150‑1 (MQ=255)

                                                                                              aaCCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTAt                                         <  1:588008/151‑1 (MQ=255)

                                                                                                cccTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTAttg                                       <  1:322771/151‑1 (MQ=255)

                                                                                                        tctcGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTg                               >  2:53435/1‑151 (MQ=255)

                                                                                                                  gCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTa                     <  1:135779/151‑1 (MQ=255)

                                                                                                                  gCATGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTa                     <  2:426906/151‑1 (MQ=255)

                                                                                                                    aTGGGCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTAtc                   >  1:290517/1‑151 (MQ=255)

                                                                                                                      gggCTGCTACGTTGGTTATCGCCACCTGGCTCGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTAtc                   <  2:356002/149‑1 (MQ=255)

                                                                                                                        gCCGGTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTAtcgtcg               <  1:650342/151‑1 (MQ=255)

                                                                                                                           ggTACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACg            >  1:416347/1‑151 (MQ=255)

                                                                                                                             tACGGTGGTTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACg            >  1:312576/1‑149 (MQ=255)

                                                                                                                                 gtggtTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGc       <  2:342050/150‑1 (MQ=255)

                                                                                                                                    gtTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGcc   <  1:311066/151‑1 (MQ=255)

                                                                                                                                    gtTATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGc    <  2:359155/150‑1 (MQ=255)

                                                                                                                                      tATCGCCACCTGGCTGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGccc  <  2:402448/150‑1 (MQ=255)

ATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCC  >  NC_000913/1769074‑1769357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A