Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,803,248	T→C	pseudogene (155/426 nt)	arpB →	pseudogene, ankyrin repeats

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,803,248	0	T	C	100.0%	116.0 / NA	33	pseudogene (155/426 nt)	arpB	pseudogene, ankyrin repeats
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/18); total (15/18)

CAAAACGATATCATTATCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCTGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACTCATTACATTACACTATAAAAAAGAA  >  NC_000913/1803100‑1803393

cAAAACGATATCATTATCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGt                                                                                                                                                 <  2:678432/151‑1 (MQ=255)

   aaCGATATCATTATCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCAt                                                                                                                                              <  1:23618/151‑1 (MQ=255)

    aCGATATCATTATCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCGCCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATa                                                                                                                                             <  1:426896/151‑1 (MQ=255)

           cATTATCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGtt                                                                                                                                      <  2:31902/151‑1 (MQ=255)

               aTCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACt                                                                                                                                   <  1:80266/150‑1 (MQ=255)

                 cAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAg                                                                                                                                <  1:588343/151‑1 (MQ=255)

                    aaCTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGaa                                                                                                                             <  1:362810/151‑1 (MQ=255)

                      cTCATTATAAGTCTCCTCATAGATTGCACATCGATAGGGACATAACAACACCTTAATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAAtt                                                                                                                           >  2:630163/1‑151 (MQ=255)

                         aTTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTa                                                                                                                         <  2:249179/150‑1 (MQ=255)

                           tataAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCg                                                                                                                      >  1:621465/1‑151 (MQ=255)

                               aGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCaa                                                                                                                   >  2:657807/1‑150 (MQ=255)

                                           gATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGaaaa                                                                                                        <  2:44436/149‑1 (MQ=255)

                                              tGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAatat                                                                                                    <  2:543399/150‑1 (MQ=255)

                                               gCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATc                                                                                                   <  1:160326/150‑1 (MQ=255)

                                                         aGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGtt                                                                                        >  1:326818/1‑151 (MQ=255)

                                                          gCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGtt                                                                                        >  2:554169/1‑150 (MQ=255)

                                                                        ccTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACCTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCtt                                                                          <  1:321784/150‑1 (MQ=255)

                                                                           tcatcaGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCttttt                                                                       >  1:406899/1‑150 (MQ=255)

                                                                               caGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCAt                                                                  >  1:673827/1‑151 (MQ=255)

                                                                                       aTTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACttt                                                           <  2:619606/150‑1 (MQ=255)

                                                                                       aTTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTg                                                          <  2:554346/151‑1 (MQ=255)

                                                                                             cAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACtt                                                    <  2:569603/151‑1 (MQ=255)

                                                                                                tttGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCAc                                                 >  2:504776/1‑151 (MQ=255)

                                                                                                      cgcCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACccatcc                                           >  1:538271/1‑151 (MQ=255)

                                                                                                         cAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACccatccat                                         >  1:19679/1‑150 (MQ=255)

                                                                                                           gCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACa                                      <  1:552746/151‑1 (MQ=255)

                                                                                                               atcaCCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGaa                                   >  2:569666/1‑150 (MQ=255)

                                                                                                                  aCCCTACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAat                                >  2:156303/1‑150 (MQ=255)

                                                                                                                      tACTTGATACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACt                           >  2:244468/1‑151 (MQ=255)

                                                                                                                            aTACCTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACTcattac                     <  2:490161/151‑1 (MQ=255)

                                                                                                                               ccTCTGACTTAAGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACTcattacatt                  >  1:608691/1‑151 (MQ=255)

                                                                                                                                         aaGTTCGATGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACTCATTACATTACACTATaaa        <  1:470176/151‑1 (MQ=255)

                                                                                                                                                aTGCCGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACTCATTACATTACACTATAAAAAAGaa  >  1:491837/1‑150 (MQ=255)

CAAAACGATATCATTATCAGAACTCATTATAAGTCTCCTCATAGATTGCACATCGATAGCGACATACCAACACCTTCATCAGAGCCTATTAATCAATTTGCGCGCCAGCTCATCACCCTACTTGATACCTCTGACTTAAGTTCGATGCTGTCATACTGTGTTACTCAGGAATTTACCGCAAACTGTCGAAAAATATCACAAAATTGTTATTCCACTGCCCTTTTTACCATTAACTTTGCCACTTCACCCATCCATACAGAAAATATACTCATTACATTACACTATAAAAAAGAA  >  NC_000913/1803100‑1803393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A