Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,129,612	G→A	L13L (CTC→CTT)	wcaE ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,129,612	0	G	A	100.0%	192.0 / NA	50	L13L (CTC→CTT)	wcaE	putative glycosyl transferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/31); total (19/31)

AAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGC  >  NC_000913/2129465‑2129754

aaaGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAgg                                                                                                                                             >  1:125371/1‑151 (MQ=255)

  aGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGtt                                                                                                                                           <  1:467964/151‑1 (MQ=255)

    cGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTAc                                                                                                                                         <  2:451686/151‑1 (MQ=255)

    cGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTAc                                                                                                                                         <  1:292730/151‑1 (MQ=255)

       aGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGaa                                                                                                                                      <  1:493838/151‑1 (MQ=255)

       aGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGa                                                                                                                                       >  1:324498/1‑150 (MQ=255)

         gTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTTCCGTCGTTGGAACCGCCATCGACAACAATCCATTCTAAGCTGATATCTTCCTCCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGaa                                                                                                                                      <  1:147913/149‑1 (MQ=255)

         gTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAAc                                                                                                                                    <  1:513867/151‑1 (MQ=255)

                aTACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATGGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGAc                                                                                                                               >  2:645461/1‑149 (MQ=255)

                aTACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGAc                                                                                                                               >  1:326500/1‑149 (MQ=255)

                       tGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTAt                                                                                                                      >  1:355092/1‑151 (MQ=255)

                         agaTTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGc                                                                                                                    >  2:179093/1‑151 (MQ=255)

                            ttttCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTa                                                                                                                  >  1:428899/1‑150 (MQ=255)

                             tttCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTaa                                                                                                                 <  1:373456/150‑1 (MQ=255)

                                ccAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCaa                                                                                                             <  1:619311/151‑1 (MQ=255)

                                  aGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAAc                                                                                                            >  2:318030/1‑150 (MQ=255)

                                  aGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAAc                                                                                                            >  2:636201/1‑150 (MQ=255)

                                  aGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACa                                                                                                           <  2:310032/151‑1 (MQ=255)

                                        tCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGttct                                                                                                     >  1:606775/1‑151 (MQ=255)

                                          aCGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGttctta                                                                                                   <  2:45398/151‑1 (MQ=255)

                                           cGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGttcttat                                                                                                  >  2:141219/1‑151 (MQ=255)

                                                 ccGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTAc                                                                                            <  2:379475/151‑1 (MQ=255)

                                                 ccGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTAc                                                                                            <  1:420944/151‑1 (MQ=255)

                                                        tGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTa                                                                                      <  1:368175/150‑1 (MQ=255)

                                                        tGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTa                                                                                      >  2:193161/1‑150 (MQ=255)

                                                         ggAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTaa                                                                                     <  1:522720/150‑1 (MQ=255)

                                                         ggAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAat                                                                                    <  2:136358/151‑1 (MQ=255)

                                                           aaCCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAatat                                                                                  <  1:284668/151‑1 (MQ=255)

                                                                    cGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCtt                                                                         <  2:346627/151‑1 (MQ=255)

                                                                      acaacaATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCtttt                                                                       <  1:605071/151‑1 (MQ=255)

                                                                              ccATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAAt                                                               <  2:648605/151‑1 (MQ=255)

                                                                               cATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAAt                                                               >  2:32584/1‑150 (MQ=255)

                                                                                   cGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACg                                                          >  1:293153/1‑151 (MQ=255)

                                                                                    gAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACgg                                                         >  1:455572/1‑151 (MQ=255)

                                                                                          gATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGAc                                                   >  1:143997/1‑151 (MQ=255)

                                                                                                 tCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGCCAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTaa                                             >  2:343949/1‑150 (MQ=255)

                                                                                                  ccACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTaaaa                                           <  1:360179/151‑1 (MQ=255)

                                                                                                  ccACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTaaa                                            <  2:16158/150‑1 (MQ=255)

                                                                                                   cACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTaaaaa                                          <  1:224147/151‑1 (MQ=255)

                                                                                                        gcgcCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGc                                       <  1:424761/149‑1 (MQ=255)

                                                                                                         cgcCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCata                                    <  1:658289/151‑1 (MQ=255)

                                                                                                         cgcCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCat                                     >  2:583938/1‑150 (MQ=255)

                                                                                                            cAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTc                                 <  1:161183/151‑1 (MQ=255)

                                                                                                                          aGGCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCg                   >  2:98746/1‑151 (MQ=255)

                                                                                                                           ggCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGa                  <  2:350512/151‑1 (MQ=255)

                                                                                                                            gCATGTGTTTTGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGaa                 <  1:509355/151‑1 (MQ=255)

                                                                                                                                   ttttGACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTa           <  1:162041/150‑1 (MQ=255)

                                                                                                                                       gACTATCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTaaaaaa      <  1:362186/151‑1 (MQ=255)

                                                                                                                                           aTCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGc  <  2:312973/151‑1 (MQ=255)

                                                                                                                                            tCCCTTCAAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGc  <  2:585569/150‑1 (MQ=255)

AAAGCGTAGGTTAAAGATACCATTGAGATTTTCCAGATACTCACGGGTGCCGTCGTTGGAACCGCCATCGACAACAATCCATTCGAAGCTGATATCTTCCACCTGCGCCAGATGCGCCAGCGAGGCATGTGTTTTGACTATCCCTTCGAGGTTACGAAACGCGACAGTGATTATGCTAAGCAACATGTTCTTATTCTTACCTCGTAATATTTAACGCTTTTCGCAAAATAAACGGACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGC  >  NC_000913/2129465‑2129754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A