Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,435,409	C→T	A76A (GCG→GCA)	truA ←	tRNA pseudouridine(38‑40) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,435,409	0	C	T	100.0%	145.3 / NA	38	A76A (GCG→GCA)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/21); total (17/21)

TGATTGTAGATGATGTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGT  >  NC_000913/2435263‑2435556

tgatTGTAGATGATGTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGCCATGAAAATCATCAGGTACAGTTTTAACACAACGCACAGAGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGACCACTCCGCGTCTTTGCGCAGTGCgg                                                                                                                                                 >  2:538738/1‑151 (MQ=255)

    tGTAGCTGATGTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCCCCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGgttg                                                                                                                                              <  2:268049/150‑1 (MQ=255)

           gatgTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGaa                                                                                                                                       <  1:620524/150‑1 (MQ=255)

            atgTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAAt                                                                                                                                     >  2:14273/1‑151 (MQ=255)

            atgTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAAt                                                                                                                                     <  1:501422/151‑1 (MQ=255)

             tgTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATg                                                                                                                                    >  2:566700/1‑151 (MQ=255)

              gTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGc                                                                                                                                   <  1:243525/151‑1 (MQ=255)

               tAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGc                                                                                                                                   >  1:292074/1‑150 (MQ=255)

                 gCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGcac                                                                                                                                 >  1:342068/1‑150 (MQ=255)

                    aTAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAAcc                                                                                                                             <  1:467137/151‑1 (MQ=255)

                      aaCGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTg                                                                                                                           <  1:536196/151‑1 (MQ=255)

                                cGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGACCGGTACCg                                                                                                                 >  2:222703/1‑151 (MQ=255)

                                  tGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCgtg                                                                                                               >  1:361402/1‑151 (MQ=255)

                                         aaaTCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTAcccc                                                                                                         >  2:648648/1‑150 (MQ=255)

                                         aaaTCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCt                                                                                                        <  1:523490/151‑1 (MQ=255)

                                          aaTCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTg                                                                                                       >  2:165568/1‑151 (MQ=255)

                                             cGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGt                                                                                                    <  1:612101/151‑1 (MQ=255)

                                             cGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGt                                                                                                    <  1:507209/151‑1 (MQ=255)

                                                          catcaGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACACCTGCGTCAGTACGCCCgg                                                                                        <  1:351266/150‑1 (MQ=255)

                                                             caGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCa                                                                                    <  1:124804/151‑1 (MQ=255)

                                                                gTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCaga                                                                                  >  2:242993/1‑150 (MQ=255)

                                                                   cAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGAc                                                                              >  2:330534/1‑151 (MQ=255)

                                                                         tAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGAt                                                                        >  1:519601/1‑151 (MQ=255)

                                                                          aaCCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGTCGGTGa                                                                         <  2:370566/149‑1 (MQ=255)

                                                                           aCCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATgg                                                                      >  2:659245/1‑151 (MQ=255)

                                                                             ccAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGgt                                                                    <  1:575693/151‑1 (MQ=255)

                                                                              cAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGgtt                                                                   <  2:314134/151‑1 (MQ=255)

                                                                                aCGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGgttcg                                                                 <  2:232643/151‑1 (MQ=255)

                                                                                  gCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGgttcgtt                                                               <  2:153745/151‑1 (MQ=255)

                                                                                        cGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCAc                                                         >  2:257784/1‑151 (MQ=255)

                                                                                          aTGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCt                                                       <  1:350161/151‑1 (MQ=255)

                                                                                                   ggTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAgcg                                              >  2:492492/1‑151 (MQ=255)

                                                                                                       aaTTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCtt                                          >  2:48384/1‑151 (MQ=255)

                                                                                                                     cccAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCt                            <  1:607178/151‑1 (MQ=255)

                                                                                                                         aGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGcac                        <  1:45539/151‑1 (MQ=255)

                                                                                                                         aGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTGCGAAATGCACAGCCTGCCCGGTACCGTGTACTCGTGCGTCAGTACGCCTGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGca                         <  1:352875/150‑1 (MQ=255)

                                                                                                                                cgccgcGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGCTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCgg                 >  2:408204/1‑151 (MQ=255)

                                                                                                                                               cAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGt  <  2:20368/151‑1 (MQ=255)

TGATTGTAGATGATGTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGT  >  NC_000913/2435263‑2435556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A